Vibrio phage BBMuffin

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Ermolyevavirinae; Cetovirus; unclassified Cetovirus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 183 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M9Z0P9|A0A6M9Z0P9_9CAUD dUTP diphosphatase OS=Vibrio phage BBMuffin OX=2736286 GN=BBMUFFIN_42 PE=4 SV=1
MM1 pKa = 7.13VKK3 pKa = 10.55VIITFGCYY11 pKa = 10.13LEE13 pKa = 4.99TCHH16 pKa = 7.0EE17 pKa = 4.31MVLVTNRR24 pKa = 11.84DD25 pKa = 3.48TLTEE29 pKa = 4.16AEE31 pKa = 4.05HH32 pKa = 7.16DD33 pKa = 4.33KK34 pKa = 11.26INSEE38 pKa = 4.19VVYY41 pKa = 10.08PMAIEE46 pKa = 4.27TTDD49 pKa = 2.79GWVGSNGFCEE59 pKa = 4.76DD60 pKa = 4.15LDD62 pKa = 3.98DD63 pKa = 6.27AEE65 pKa = 5.79DD66 pKa = 3.58IEE68 pKa = 4.75EE69 pKa = 4.22AVEE72 pKa = 5.21DD73 pKa = 3.63EE74 pKa = 4.06AFYY77 pKa = 11.03YY78 pKa = 10.6SEE80 pKa = 4.63VYY82 pKa = 10.55DD83 pKa = 4.08PEE85 pKa = 4.21KK86 pKa = 10.81HH87 pKa = 7.24DD88 pKa = 4.17GFLMGCQDD96 pKa = 3.06EE97 pKa = 4.33WQIIEE102 pKa = 4.48EE103 pKa = 4.27IEE105 pKa = 3.94LL106 pKa = 3.79

Molecular weight: 12.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M9Z0M7|A0A6M9Z0M7_9CAUD Uncharacterized protein OS=Vibrio phage BBMuffin OX=2736286 GN=BBMUFFIN_203 PE=4 SV=1
MM1 pKa = 7.0GQIAFSKK8 pKa = 10.19LSRR11 pKa = 11.84HH12 pKa = 5.62SMHH15 pKa = 6.61HH16 pKa = 6.79CIPSYY21 pKa = 9.91CLKK24 pKa = 10.51EE25 pKa = 3.97RR26 pKa = 11.84VPLGTLLVSQKK37 pKa = 9.54PLSVSTTFFNFFRR50 pKa = 11.84RR51 pKa = 11.84AFQAFLSRR59 pKa = 11.84RR60 pKa = 11.84VVAVWVYY67 pKa = 10.39NSEE70 pKa = 3.88SRR72 pKa = 11.84IIVV75 pKa = 3.35

Molecular weight: 8.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

183

0

183

35945

36

1018

196.4

22.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.715 ± 0.322

1.288 ± 0.087

6.349 ± 0.149

6.994 ± 0.228

3.987 ± 0.139

6.474 ± 0.19

2.061 ± 0.124

6.104 ± 0.141

7.236 ± 0.171

8.438 ± 0.198

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.765 ± 0.108

5.074 ± 0.149

3.461 ± 0.146

3.547 ± 0.133

4.326 ± 0.132

6.198 ± 0.225

6.151 ± 0.194

6.541 ± 0.137

1.341 ± 0.084

3.95 ± 0.128
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski