Pedobacter sp. ok626

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter; unclassified Pedobacter

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5460 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G9E4H9|A0A1G9E4H9_9SPHI Type IX secretion system membrane protein PorP/SprF family OS=Pedobacter sp. ok626 OX=1761882 GN=SAMN04487898_11115 PE=4 SV=1
MM1 pKa = 7.86VIIQGEE7 pKa = 4.19YY8 pKa = 10.24SGTQNFVCLDD18 pKa = 3.26ISFLEE23 pKa = 4.07FLQMAFLYY31 pKa = 10.41RR32 pKa = 11.84DD33 pKa = 3.97LEE35 pKa = 4.22NMLDD39 pKa = 3.52EE40 pKa = 4.84WDD42 pKa = 4.92DD43 pKa = 3.98GLDD46 pKa = 3.46EE47 pKa = 4.42EE48 pKa = 6.0KK49 pKa = 10.28IDD51 pKa = 4.4GFKK54 pKa = 10.93KK55 pKa = 10.26EE56 pKa = 3.91INTIKK61 pKa = 10.81SNLYY65 pKa = 9.26EE66 pKa = 4.82LYY68 pKa = 10.06PFQYY72 pKa = 10.63FF73 pKa = 3.29

Molecular weight: 8.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9LHJ8|A0A1G9LHJ8_9SPHI Cysteine desulfurase IscS OS=Pedobacter sp. ok626 OX=1761882 GN=iscS PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.54RR3 pKa = 11.84TFQPSQRR10 pKa = 11.84KK11 pKa = 8.72RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.3HH16 pKa = 3.94GFRR19 pKa = 11.84EE20 pKa = 4.19RR21 pKa = 11.84MATANGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.54GRR39 pKa = 11.84KK40 pKa = 8.64RR41 pKa = 11.84LTVSSEE47 pKa = 3.76GRR49 pKa = 11.84HH50 pKa = 4.97KK51 pKa = 11.04AA52 pKa = 3.25

Molecular weight: 6.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5460

0

5460

2081436

14

7856

381.2

42.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.425 ± 0.036

0.723 ± 0.009

5.311 ± 0.022

5.503 ± 0.034

4.842 ± 0.022

6.914 ± 0.042

1.644 ± 0.016

7.211 ± 0.028

7.297 ± 0.037

9.508 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.259 ± 0.018

5.96 ± 0.034

3.75 ± 0.018

3.593 ± 0.019

3.77 ± 0.024

6.582 ± 0.027

5.957 ± 0.047

6.294 ± 0.028

1.189 ± 0.012

4.269 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski