Erwinia phage vB_EamM_EarlPhillipIV

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Derbicusvirus; unclassified Derbicusvirus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 241 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B2ICC7|A0A1B2ICC7_9CAUD Uncharacterized protein OS=Erwinia phage vB_EamM_EarlPhillipIV OX=1883372 GN=EARLPHILLIPIV_69 PE=4 SV=1
MM1 pKa = 6.87TRR3 pKa = 11.84VMYY6 pKa = 10.64SAMDD10 pKa = 5.09AIHH13 pKa = 7.26AYY15 pKa = 10.2GIEE18 pKa = 4.19SFAALIRR25 pKa = 11.84EE26 pKa = 4.05NLVVGLDD33 pKa = 3.73GVNVEE38 pKa = 4.76VFDD41 pKa = 4.36DD42 pKa = 3.98ANFYY46 pKa = 10.92RR47 pKa = 11.84VFAYY51 pKa = 10.14AYY53 pKa = 9.23IAGAMSGSSQVFLEE67 pKa = 4.38AFGVDD72 pKa = 3.2NVEE75 pKa = 4.34PLDD78 pKa = 4.14LQTGPLVVNWNPPGQPEE95 pKa = 4.3DD96 pKa = 3.55VVGFTLGDD104 pKa = 4.15DD105 pKa = 4.64DD106 pKa = 6.2PNDD109 pKa = 5.09LIDD112 pKa = 4.17NDD114 pKa = 3.9FSTFILPFTIDD125 pKa = 3.08STEE128 pKa = 4.08ADD130 pKa = 3.7TVEE133 pKa = 4.07VTSRR137 pKa = 11.84YY138 pKa = 9.74VEE140 pKa = 4.13LLNKK144 pKa = 9.96EE145 pKa = 4.53LFSQTGLVSVIKK157 pKa = 10.97SMFPGKK163 pKa = 10.3YY164 pKa = 8.93VDD166 pKa = 3.79YY167 pKa = 11.05RR168 pKa = 11.84GDD170 pKa = 3.56DD171 pKa = 3.86LFTMVVVCDD180 pKa = 3.64VV181 pKa = 3.27

Molecular weight: 19.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B2ICS9|A0A1B2ICS9_9CAUD Uncharacterized protein OS=Erwinia phage vB_EamM_EarlPhillipIV OX=1883372 GN=EARLPHILLIPIV_166 PE=4 SV=1
MM1 pKa = 6.19TTAFVSAILVIWLCVAFGLRR21 pKa = 11.84HH22 pKa = 4.81SVGIIRR28 pKa = 11.84AGEE31 pKa = 3.92VAIGRR36 pKa = 11.84TKK38 pKa = 10.52EE39 pKa = 3.78QLNRR43 pKa = 11.84KK44 pKa = 8.97ALTLVVFTISTSAGMTYY61 pKa = 11.24VMTQIYY67 pKa = 9.61FLHH70 pKa = 5.46QTTLQLAGNMM80 pKa = 4.16

Molecular weight: 8.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

241

0

241

71303

27

2479

295.9

33.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.171 ± 0.164

0.853 ± 0.051

6.446 ± 0.107

6.129 ± 0.155

4.101 ± 0.092

6.503 ± 0.203

1.982 ± 0.091

5.571 ± 0.086

5.398 ± 0.138

9.011 ± 0.115

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.78 ± 0.084

4.924 ± 0.107

4.276 ± 0.102

3.66 ± 0.092

5.54 ± 0.152

5.897 ± 0.107

6.495 ± 0.175

7.113 ± 0.127

1.327 ± 0.054

3.825 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski