Hubei sobemo-like virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KF00|A0A1L3KF00_9VIRU Capsid protein OS=Hubei sobemo-like virus 3 OX=1923216 PE=3 SV=1
MM1 pKa = 7.54EE2 pKa = 5.68GDD4 pKa = 4.04DD5 pKa = 4.66VKK7 pKa = 11.3EE8 pKa = 4.35CSTKK12 pKa = 10.01VAEE15 pKa = 4.66MYY17 pKa = 10.03QAAGVAWDD25 pKa = 3.65FAFRR29 pKa = 11.84SYY31 pKa = 9.63EE32 pKa = 3.93TFAEE36 pKa = 4.29DD37 pKa = 4.49FEE39 pKa = 4.52NAYY42 pKa = 9.9EE43 pKa = 4.83SNVCHH48 pKa = 6.92IKK50 pKa = 8.69PTSGPGYY57 pKa = 9.14PYY59 pKa = 11.08RR60 pKa = 11.84LFGDD64 pKa = 3.73EE65 pKa = 3.84NRR67 pKa = 11.84EE68 pKa = 3.8ILEE71 pKa = 4.06SHH73 pKa = 5.82GAEE76 pKa = 3.87IKK78 pKa = 10.64RR79 pKa = 11.84LTKK82 pKa = 9.69MRR84 pKa = 11.84LEE86 pKa = 4.45TILFGGHH93 pKa = 6.34DD94 pKa = 4.14FEE96 pKa = 7.92SIAKK100 pKa = 10.34DD101 pKa = 3.25PMQWLEE107 pKa = 3.98KK108 pKa = 10.51GLRR111 pKa = 11.84DD112 pKa = 4.31PDD114 pKa = 3.42RR115 pKa = 11.84LFPKK119 pKa = 10.33NQANPLRR126 pKa = 11.84KK127 pKa = 9.14PLPRR131 pKa = 11.84VIAGSSLVDD140 pKa = 3.35QLVTRR145 pKa = 11.84ILFSGFTDD153 pKa = 3.91AEE155 pKa = 4.45GEE157 pKa = 4.31AYY159 pKa = 9.66PFLPTKK165 pKa = 10.41KK166 pKa = 10.51GIGFSDD172 pKa = 3.3EE173 pKa = 4.05HH174 pKa = 8.58AEE176 pKa = 3.89LLGEE180 pKa = 4.2QFEE183 pKa = 4.89GLNKK187 pKa = 10.5ALGRR191 pKa = 11.84PPAVSDD197 pKa = 3.47VAGWEE202 pKa = 4.4KK203 pKa = 11.02NFSEE207 pKa = 4.65PVAEE211 pKa = 4.53CTRR214 pKa = 11.84IPMKK218 pKa = 10.07EE219 pKa = 4.14TMKK222 pKa = 10.75SGSKK226 pKa = 10.21EE227 pKa = 3.83LFDD230 pKa = 5.66VGFNWWKK237 pKa = 10.55FSLLSNLAVTDD248 pKa = 3.2SGKK251 pKa = 10.39LVRR254 pKa = 11.84FKK256 pKa = 10.81DD257 pKa = 3.64LKK259 pKa = 9.58VQRR262 pKa = 11.84SGNFLTTTSNGIGRR276 pKa = 11.84KK277 pKa = 8.89CVALSVGSVANTAGDD292 pKa = 4.59DD293 pKa = 3.7CHH295 pKa = 6.58EE296 pKa = 4.34WNDD299 pKa = 3.63LSVEE303 pKa = 4.11EE304 pKa = 5.47LIQAYY309 pKa = 10.04ARR311 pKa = 11.84IGVPVRR317 pKa = 11.84DD318 pKa = 3.94VVQMDD323 pKa = 3.57SKK325 pKa = 10.0TLVFCSHH332 pKa = 6.24SFQRR336 pKa = 11.84DD337 pKa = 2.55GDD339 pKa = 4.18GRR341 pKa = 11.84WKK343 pKa = 10.43CWLSEE348 pKa = 4.19WEE350 pKa = 4.06RR351 pKa = 11.84MLYY354 pKa = 9.34EE355 pKa = 4.32ASRR358 pKa = 11.84SKK360 pKa = 11.06LLDD363 pKa = 3.24VGTDD367 pKa = 3.6LNWIKK372 pKa = 10.4EE373 pKa = 4.44VEE375 pKa = 4.13NHH377 pKa = 7.25PDD379 pKa = 3.07PEE381 pKa = 4.14MRR383 pKa = 11.84RR384 pKa = 11.84KK385 pKa = 9.56FHH387 pKa = 6.69AFVSGRR393 pKa = 11.84RR394 pKa = 11.84LLLGAVAEE402 pKa = 4.12HH403 pKa = 7.34DD404 pKa = 3.95EE405 pKa = 4.33VSEE408 pKa = 4.46SGSSHH413 pKa = 6.53PAEE416 pKa = 4.47KK417 pKa = 10.81GCAKK421 pKa = 10.35RR422 pKa = 11.84NEE424 pKa = 4.36SEE426 pKa = 3.94

Molecular weight: 47.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KF02|A0A1L3KF02_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 3 OX=1923216 PE=4 SV=1
MM1 pKa = 7.27TKK3 pKa = 10.12SANRR7 pKa = 11.84GAATPQKK14 pKa = 10.29KK15 pKa = 9.38AAQRR19 pKa = 11.84GMKK22 pKa = 9.8VSNKK26 pKa = 9.24PNPIRR31 pKa = 11.84KK32 pKa = 7.86KK33 pKa = 8.72QKK35 pKa = 7.69RR36 pKa = 11.84TRR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84NRR42 pKa = 11.84PAQVGLAYY50 pKa = 9.51ATGSSAKK57 pKa = 7.63MTSIVVEE64 pKa = 3.96KK65 pKa = 9.56TEE67 pKa = 3.94RR68 pKa = 11.84LGTIVSPATASGVVLTQYY86 pKa = 11.12VNAGNLALSTSSYY99 pKa = 10.67LGRR102 pKa = 11.84QAALFDD108 pKa = 4.2KK109 pKa = 10.82YY110 pKa = 10.83QFSKK114 pKa = 11.37FEE116 pKa = 3.88IEE118 pKa = 4.11YY119 pKa = 10.39VPIVSASTSGNVIIGMDD136 pKa = 4.6LSPDD140 pKa = 3.44DD141 pKa = 4.87AAPGDD146 pKa = 3.89ASGMTNLSLGYY157 pKa = 10.59AEE159 pKa = 4.49GNAWRR164 pKa = 11.84NFKK167 pKa = 10.44YY168 pKa = 10.21AAQCFACFPAGPKK181 pKa = 9.82FVRR184 pKa = 11.84STTEE188 pKa = 3.44QLGTNASLFDD198 pKa = 3.78MGSLYY203 pKa = 10.31IFTEE207 pKa = 4.68GAPVNTTLGYY217 pKa = 10.04IDD219 pKa = 3.4VHH221 pKa = 6.51YY222 pKa = 10.1KK223 pKa = 9.78VHH225 pKa = 5.71LHH227 pKa = 5.3GVNRR231 pKa = 11.84NANDD235 pKa = 3.4SPAGLLRR242 pKa = 11.84PAALATLSPTSTSAIVPGGTYY263 pKa = 9.01ATTTFASFRR272 pKa = 11.84GFSPSGIGQSDD283 pKa = 3.93MVFSAVTPAEE293 pKa = 4.24VYY295 pKa = 9.19GTVGNYY301 pKa = 6.92ITYY304 pKa = 7.63GTNSITLNAGTYY316 pKa = 7.75SVKK319 pKa = 9.84LTSCVCSDD327 pKa = 3.83TYY329 pKa = 11.79SNGLVRR335 pKa = 11.84LKK337 pKa = 10.67RR338 pKa = 11.84DD339 pKa = 3.5GNVLLSNAHH348 pKa = 5.34GWGSLSLPTTAYY360 pKa = 9.85VQTGSVDD367 pKa = 3.57SIEE370 pKa = 4.58RR371 pKa = 11.84SFTVTTTSTLTLDD384 pKa = 3.32TFVFYY389 pKa = 10.92SATPYY394 pKa = 8.76TQNKK398 pKa = 5.42TWNVVYY404 pKa = 11.02VNGDD408 pKa = 3.73SVPSTFITLTRR419 pKa = 11.84LAAA422 pKa = 4.34

Molecular weight: 44.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1618

422

770

539.3

58.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.703 ± 0.959

1.422 ± 0.217

4.017 ± 0.83

5.871 ± 1.695

4.079 ± 0.492

7.54 ± 0.493

1.731 ± 0.335

3.523 ± 0.174

6.675 ± 1.02

7.417 ± 0.47

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.607 ± 0.199

3.708 ± 0.833

4.944 ± 0.225

3.152 ± 0.451

4.697 ± 0.51

9.642 ± 0.607

8.529 ± 1.782

7.046 ± 0.315

1.731 ± 0.415

2.967 ± 0.723
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski