Stenotrophomonas phage vB_SmaS-DLP_6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; unclassified Ackermannviridae

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 241 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142F015|A0A142F015_9CAUD Uncharacterized protein OS=Stenotrophomonas phage vB_SmaS-DLP_6 OX=1651816 GN=AAY80_245 PE=4 SV=1
MM1 pKa = 8.03IEE3 pKa = 3.75FRR5 pKa = 11.84EE6 pKa = 3.98FDD8 pKa = 3.72RR9 pKa = 11.84NDD11 pKa = 2.76WMSFAGCEE19 pKa = 3.89NGPNGEE25 pKa = 4.32PPLIAEE31 pKa = 4.4TEE33 pKa = 3.77FDD35 pKa = 3.75GFDD38 pKa = 3.8TIVVADD44 pKa = 4.07ANGVCICMVQQDD56 pKa = 3.63GDD58 pKa = 3.8EE59 pKa = 4.62YY60 pKa = 10.88IDD62 pKa = 5.74RR63 pKa = 11.84NYY65 pKa = 9.26TWNHH69 pKa = 6.15DD70 pKa = 3.63NALPYY75 pKa = 9.89AANVLFAKK83 pKa = 10.68GITDD87 pKa = 4.55FSHH90 pKa = 7.95DD91 pKa = 3.97ALTALGFFEE100 pKa = 4.59VV101 pKa = 4.01

Molecular weight: 11.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142F090|A0A142F090_9CAUD Uncharacterized protein OS=Stenotrophomonas phage vB_SmaS-DLP_6 OX=1651816 GN=AAY80_247 PE=4 SV=1
MM1 pKa = 7.02KK2 pKa = 10.38RR3 pKa = 11.84EE4 pKa = 4.11IKK6 pKa = 10.26RR7 pKa = 11.84HH8 pKa = 4.99IRR10 pKa = 11.84LGDD13 pKa = 3.58CCPGHH18 pKa = 7.24DD19 pKa = 4.53KK20 pKa = 11.28YY21 pKa = 11.52PNDD24 pKa = 3.32SYY26 pKa = 12.08ANNRR30 pKa = 11.84SKK32 pKa = 10.75RR33 pKa = 11.84ARR35 pKa = 11.84ARR37 pKa = 11.84GIAKK41 pKa = 7.42EE42 pKa = 3.87HH43 pKa = 5.36RR44 pKa = 11.84HH45 pKa = 4.81ARR47 pKa = 11.84RR48 pKa = 11.84LVRR51 pKa = 11.84QEE53 pKa = 4.0MIHH56 pKa = 6.11AVSEE60 pKa = 4.41AVV62 pKa = 3.02

Molecular weight: 7.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

241

0

241

49729

32

1824

206.3

23.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.4 ± 0.194

0.943 ± 0.06

6.377 ± 0.148

6.383 ± 0.212

4.327 ± 0.112

6.885 ± 0.236

2.091 ± 0.151

5.683 ± 0.114

6.624 ± 0.306

7.812 ± 0.16

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.699 ± 0.112

4.832 ± 0.206

3.982 ± 0.125

3.549 ± 0.099

5.138 ± 0.129

5.916 ± 0.167

6.248 ± 0.39

6.899 ± 0.15

1.341 ± 0.071

3.871 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski