Mycobacterium phage JacoRen57

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Mapvirus; unclassified Mapvirus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S9U8R2|A0A3S9U8R2_9CAUD Uncharacterized protein OS=Mycobacterium phage JacoRen57 OX=2499043 GN=32 PE=4 SV=1
MM1 pKa = 6.27TTNEE5 pKa = 3.53ISHH8 pKa = 5.87YY9 pKa = 5.7MTRR12 pKa = 11.84VNEE15 pKa = 3.65LRR17 pKa = 11.84RR18 pKa = 11.84PYY20 pKa = 10.62NEE22 pKa = 3.86RR23 pKa = 11.84LRR25 pKa = 11.84AFVNRR30 pKa = 11.84PGPRR34 pKa = 11.84IVHH37 pKa = 6.2PFVTDD42 pKa = 3.37TLAALIDD49 pKa = 5.04LIGSMSDD56 pKa = 3.55DD57 pKa = 4.15AVDD60 pKa = 4.3EE61 pKa = 4.23YY62 pKa = 11.47QIAIYY67 pKa = 10.07AAIDD71 pKa = 3.69GPEE74 pKa = 4.33FIEE77 pKa = 4.06EE78 pKa = 3.86QAACGYY84 pKa = 11.13DD85 pKa = 3.13NDD87 pKa = 4.87AFADD91 pKa = 4.01PEE93 pKa = 4.28SAEE96 pKa = 3.66YY97 pKa = 9.93RR98 pKa = 11.84AYY100 pKa = 9.47CTEE103 pKa = 3.55VDD105 pKa = 3.73TYY107 pKa = 11.58LAIHH111 pKa = 5.88NHH113 pKa = 5.09LSEE116 pKa = 4.51FEE118 pKa = 4.23RR119 pKa = 11.84GLLSSIVDD127 pKa = 3.69VQGLIDD133 pKa = 5.77DD134 pKa = 4.02NTEE137 pKa = 3.59IDD139 pKa = 3.53VDD141 pKa = 4.0SALIAWFEE149 pKa = 4.0RR150 pKa = 11.84VMDD153 pKa = 4.89TIEE156 pKa = 3.83NLL158 pKa = 3.43

Molecular weight: 17.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S9U8J9|A0A3S9U8J9_9CAUD MerR-like helix-turn-helix DNA binding domain protein OS=Mycobacterium phage JacoRen57 OX=2499043 GN=31 PE=4 SV=1
MM1 pKa = 6.64TRR3 pKa = 11.84VYY5 pKa = 10.02VAPARR10 pKa = 11.84PPIDD14 pKa = 3.32KK15 pKa = 10.33SIRR18 pKa = 11.84GVEE21 pKa = 4.02TRR23 pKa = 11.84SPSEE27 pKa = 3.89NEE29 pKa = 3.6MLEE32 pKa = 4.47IVCGMLPQFFFHH44 pKa = 6.08WQSPLSRR51 pKa = 11.84LWRR54 pKa = 11.84SGDD57 pKa = 3.22PAFCKK62 pKa = 10.38RR63 pKa = 11.84FGKK66 pKa = 8.61TGRR69 pKa = 11.84VMMLTT74 pKa = 3.67

Molecular weight: 8.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

16348

47

1473

223.9

24.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.487 ± 0.429

0.801 ± 0.124

6.57 ± 0.21

6.478 ± 0.268

3.358 ± 0.148

7.817 ± 0.422

1.939 ± 0.183

5.034 ± 0.206

5.157 ± 0.276

7.86 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.551 ± 0.139

3.976 ± 0.182

5.854 ± 0.273

3.878 ± 0.183

6.362 ± 0.359

5.365 ± 0.209

5.897 ± 0.326

6.527 ± 0.218

2.227 ± 0.128

2.863 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski