Moosepox virus GoldyGopher14

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Chitovirales; Poxviridae; Chordopoxvirinae; Cervidpoxvirus; unclassified Cervidpoxvirus

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 165 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S7SW86|A0A3S7SW86_9POXV EEV glycoprotein OS=Moosepox virus GoldyGopher14 OX=2340996 PE=4 SV=1
MM1 pKa = 7.81GITHH5 pKa = 6.97EE6 pKa = 4.41LDD8 pKa = 2.88ILIVSEE14 pKa = 4.17NLSLKK19 pKa = 10.42NVEE22 pKa = 4.24LLKK25 pKa = 10.79GDD27 pKa = 4.4SYY29 pKa = 12.14GCLINIKK36 pKa = 10.58VNGQKK41 pKa = 9.05TFDD44 pKa = 3.32FMFILRR50 pKa = 11.84PDD52 pKa = 2.98WTEE55 pKa = 3.73VQKK58 pKa = 11.28VKK60 pKa = 10.51SIKK63 pKa = 9.38MLNNGFPVEE72 pKa = 4.04MKK74 pKa = 10.3VIKK77 pKa = 10.46SSLFEE82 pKa = 3.91VVYY85 pKa = 10.23QASFTTCFNTTLEE98 pKa = 4.09IFSDD102 pKa = 3.35NFKK105 pKa = 10.66QFKK108 pKa = 9.41EE109 pKa = 4.12KK110 pKa = 11.15YY111 pKa = 8.98PIVNINTIKK120 pKa = 10.56RR121 pKa = 11.84EE122 pKa = 3.97YY123 pKa = 10.27EE124 pKa = 3.87IVNSGMTCINLDD136 pKa = 4.15SPICDD141 pKa = 3.57EE142 pKa = 5.9DD143 pKa = 4.34RR144 pKa = 11.84IKK146 pKa = 9.36YY147 pKa = 7.58TKK149 pKa = 10.3YY150 pKa = 10.04DD151 pKa = 3.32YY152 pKa = 11.71SFIGDD157 pKa = 3.93SYY159 pKa = 12.15NEE161 pKa = 3.56IDD163 pKa = 5.51YY164 pKa = 11.1YY165 pKa = 11.16ATDD168 pKa = 3.57NEE170 pKa = 4.26EE171 pKa = 5.26DD172 pKa = 3.55SDD174 pKa = 4.85EE175 pKa = 4.95DD176 pKa = 5.07FEE178 pKa = 7.6DD179 pKa = 4.12DD180 pKa = 4.81TDD182 pKa = 5.15IEE184 pKa = 5.84DD185 pKa = 4.1DD186 pKa = 4.1TDD188 pKa = 4.38DD189 pKa = 5.27DD190 pKa = 5.06GYY192 pKa = 11.61SDD194 pKa = 5.53EE195 pKa = 5.47EE196 pKa = 5.04DD197 pKa = 3.95DD198 pKa = 4.29NN199 pKa = 4.61

Molecular weight: 23.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S7SWH2|A0A3S7SWH2_9POXV Uncharacterized protein OS=Moosepox virus GoldyGopher14 OX=2340996 PE=4 SV=1
MM1 pKa = 7.51ALSAKK6 pKa = 9.8EE7 pKa = 3.78IFSAISLTLLALLMIISGGALAFKK31 pKa = 10.54SLAPHH36 pKa = 7.17RR37 pKa = 11.84IVSMRR42 pKa = 11.84SATFNRR48 pKa = 11.84VVSILEE54 pKa = 3.78YY55 pKa = 10.57LAILIFIPGTIALYY69 pKa = 10.05SAYY72 pKa = 9.43IKK74 pKa = 10.8KK75 pKa = 10.35LFSS78 pKa = 3.42

Molecular weight: 8.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

165

0

165

50852

53

1951

308.2

35.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.83 ± 0.119

2.037 ± 0.09

6.169 ± 0.102

5.636 ± 0.153

5.311 ± 0.145

3.628 ± 0.11

1.76 ± 0.073

11.225 ± 0.222

9.036 ± 0.173

9.06 ± 0.165

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.505 ± 0.066

8.281 ± 0.159

2.971 ± 0.09

2.022 ± 0.074

3.201 ± 0.104

7.713 ± 0.147

5.435 ± 0.132

5.077 ± 0.114

0.661 ± 0.041

5.441 ± 0.108
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski