Lysinibacillus massiliensis 4400831 = CIP 108448 = CCUG 49529

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Ureibacillus; Ureibacillus massiliensis

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3499 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A3JV68|A0A0A3JV68_9BACI ABC transporter permease OS=Lysinibacillus massiliensis 4400831 = CIP 108448 = CCUG 49529 OX=1211035 GN=CD30_09335 PE=3 SV=1
MM1 pKa = 7.42VHH3 pKa = 5.84EE4 pKa = 5.32HH5 pKa = 6.11DD6 pKa = 4.66HH7 pKa = 5.08EE8 pKa = 5.68HH9 pKa = 5.29EE10 pKa = 4.49HH11 pKa = 6.04RR12 pKa = 11.84HH13 pKa = 4.21ITIVDD18 pKa = 3.37EE19 pKa = 5.17DD20 pKa = 4.39GNEE23 pKa = 3.91QLCEE27 pKa = 3.94VIYY30 pKa = 10.19TFDD33 pKa = 3.42SEE35 pKa = 4.92EE36 pKa = 3.91FGKK39 pKa = 10.83SYY41 pKa = 11.29VLYY44 pKa = 9.94SLVGAEE50 pKa = 4.94EE51 pKa = 4.46DD52 pKa = 4.45DD53 pKa = 3.82EE54 pKa = 4.6GQIEE58 pKa = 4.24IFASAFSPSEE68 pKa = 3.8NGEE71 pKa = 4.19DD72 pKa = 4.41GEE74 pKa = 4.56LEE76 pKa = 4.52PIEE79 pKa = 5.16TEE81 pKa = 4.37AEE83 pKa = 3.43WDD85 pKa = 4.14LIEE88 pKa = 4.94EE89 pKa = 4.34VLNTLEE95 pKa = 6.15DD96 pKa = 3.6EE97 pKa = 4.82FEE99 pKa = 4.99DD100 pKa = 4.6EE101 pKa = 4.67EE102 pKa = 4.43EE103 pKa = 4.07

Molecular weight: 11.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A3J4M2|A0A0A3J4M2_9BACI Uncharacterized protein OS=Lysinibacillus massiliensis 4400831 = CIP 108448 = CCUG 49529 OX=1211035 GN=CD30_02870 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.44KK9 pKa = 7.96RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.54VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.91VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3499

0

3499

1022772

37

1505

292.3

32.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.683 ± 0.041

0.722 ± 0.01

5.027 ± 0.033

7.806 ± 0.052

4.648 ± 0.038

6.482 ± 0.038

1.962 ± 0.018

8.555 ± 0.047

6.915 ± 0.039

9.658 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.645 ± 0.02

4.904 ± 0.032

3.439 ± 0.02

3.664 ± 0.026

3.797 ± 0.032

6.051 ± 0.028

5.497 ± 0.027

6.994 ± 0.031

0.933 ± 0.013

3.617 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski