Flavobacteriaceae bacterium 14752

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; unclassified Flavobacteriaceae

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2559 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A426KL53|A0A426KL53_9FLAO ATP-dependent 6-phosphofructokinase OS=Flavobacteriaceae bacterium 14752 OX=2491711 GN=pfkA PE=3 SV=1
MM1 pKa = 7.8KK2 pKa = 10.38YY3 pKa = 10.45LYY5 pKa = 10.76LAFLSILFIQFSFAQTATSNDD26 pKa = 3.53SLTLNEE32 pKa = 4.03VLEE35 pKa = 4.51FMVEE39 pKa = 4.18DD40 pKa = 4.9LDD42 pKa = 4.42CASISNITSPNNAQMHH58 pKa = 5.43NQGFSSYY65 pKa = 11.42GSFQVQNEE73 pKa = 4.17PNFPFDD79 pKa = 3.35SGIVLSTTSVSDD91 pKa = 4.64LEE93 pKa = 5.23NITNNGDD100 pKa = 3.87SQWPGDD106 pKa = 3.87SDD108 pKa = 3.96LAALIQEE115 pKa = 4.88PGNTHH120 pKa = 6.28NATVIEE126 pKa = 4.14FDD128 pKa = 4.69FIPFRR133 pKa = 11.84EE134 pKa = 4.07EE135 pKa = 3.85LKK137 pKa = 10.66VDD139 pKa = 3.47YY140 pKa = 10.83LLASDD145 pKa = 4.96EE146 pKa = 4.59YY147 pKa = 10.72PVFVCDD153 pKa = 5.03FADD156 pKa = 3.57TFAFIISGPGISNVNPYY173 pKa = 10.93DD174 pKa = 3.76HH175 pKa = 7.8DD176 pKa = 5.04ANPNTPEE183 pKa = 3.92VNLDD187 pKa = 3.52LGGLNIATLPGTTIPVNPTNIHH209 pKa = 7.43DD210 pKa = 3.96MTTDD214 pKa = 3.44CTGSMGEE221 pKa = 3.96FAVPQFFDD229 pKa = 3.7AQAFDD234 pKa = 3.9NNIISFTGQTLPLTAKK250 pKa = 10.07VDD252 pKa = 5.21LIPGQTYY259 pKa = 10.36HH260 pKa = 7.26IEE262 pKa = 4.01LKK264 pKa = 10.49IADD267 pKa = 4.35RR268 pKa = 11.84GDD270 pKa = 3.5TVLNSAVFIDD280 pKa = 3.82ADD282 pKa = 3.9SFEE285 pKa = 4.78MGTIPEE291 pKa = 4.6DD292 pKa = 3.39LPYY295 pKa = 10.97EE296 pKa = 4.28PGLPVEE302 pKa = 5.87LPEE305 pKa = 4.72CWTTSDD311 pKa = 3.45TASFDD316 pKa = 3.44ILNTCSEE323 pKa = 4.12TSEE326 pKa = 5.33NYY328 pKa = 9.59LQLYY332 pKa = 8.75GGNYY336 pKa = 9.68SLQTAAVDD344 pKa = 3.67TDD346 pKa = 3.67GVAGVNISWDD356 pKa = 3.62MLNGCNDD363 pKa = 2.82IAEE366 pKa = 4.47AGKK369 pKa = 10.06NLLVEE374 pKa = 4.19YY375 pKa = 10.53FNGNDD380 pKa = 3.27WQLLADD386 pKa = 4.87IDD388 pKa = 4.86PISIPVANSNSSDD401 pKa = 2.48NWMTVNYY408 pKa = 8.51TVTSGMNKK416 pKa = 9.48NFTLRR421 pKa = 11.84FSRR424 pKa = 11.84QSGNNQQDD432 pKa = 4.23DD433 pKa = 3.57ISIANLSITEE443 pKa = 3.96QTLSNEE449 pKa = 4.13EE450 pKa = 4.08FSVDD454 pKa = 3.13TFKK457 pKa = 10.72IYY459 pKa = 10.16PNPVSDD465 pKa = 3.57ILTIEE470 pKa = 4.45TLNSTQIDD478 pKa = 3.76QIEE481 pKa = 4.58IIDD484 pKa = 3.76IQGKK488 pKa = 8.24ILKK491 pKa = 6.88TTNQLEE497 pKa = 4.11IDD499 pKa = 4.1VQSLSTGLYY508 pKa = 7.59FAKK511 pKa = 10.33ISRR514 pKa = 11.84QNSYY518 pKa = 10.19IIKK521 pKa = 10.3RR522 pKa = 11.84FIKK525 pKa = 10.4KK526 pKa = 9.89

Molecular weight: 57.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3R8P6V5|A0A3R8P6V5_9FLAO Uncharacterized protein OS=Flavobacteriaceae bacterium 14752 OX=2491711 GN=EIG84_03770 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.2RR21 pKa = 11.84MASANGRR28 pKa = 11.84KK29 pKa = 8.84ILKK32 pKa = 9.62RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.12GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.62LSVSSEE47 pKa = 3.22TSMRR51 pKa = 11.84RR52 pKa = 11.84KK53 pKa = 9.82

Molecular weight: 6.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2559

0

2559

856683

35

2367

334.8

38.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.891 ± 0.04

0.734 ± 0.015

5.989 ± 0.044

6.469 ± 0.044

5.515 ± 0.041

5.875 ± 0.049

1.888 ± 0.024

7.993 ± 0.045

8.239 ± 0.077

9.406 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.037 ± 0.022

6.348 ± 0.051

3.369 ± 0.026

4.133 ± 0.03

3.281 ± 0.033

6.578 ± 0.042

5.308 ± 0.047

5.952 ± 0.032

1.02 ± 0.018

3.974 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski