Gordonia phage Phendrix

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Godonkavirus; unclassified Godonkavirus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 188 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514U122|A0A514U122_9CAUD Minor tail protein OS=Gordonia phage Phendrix OX=2593335 GN=86 PE=4 SV=1
MM1 pKa = 7.32YY2 pKa = 9.94DD3 pKa = 4.85CYY5 pKa = 11.45NKK7 pKa = 10.9DD8 pKa = 3.22PDD10 pKa = 3.76AVLDD14 pKa = 4.95YY15 pKa = 11.19GLEE18 pKa = 3.83WDD20 pKa = 4.11DD21 pKa = 3.54WVIGGDD27 pKa = 4.02TIVSSSWEE35 pKa = 3.9VTGTDD40 pKa = 3.0SSLVIDD46 pKa = 5.11SDD48 pKa = 3.97FQDD51 pKa = 3.48VLTTGVWLSGGTVGSLYY68 pKa = 10.42ILTNHH73 pKa = 6.75IEE75 pKa = 4.18TSAGRR80 pKa = 11.84EE81 pKa = 3.99DD82 pKa = 4.15DD83 pKa = 4.01RR84 pKa = 11.84SFSVYY89 pKa = 9.57IVEE92 pKa = 4.27RR93 pKa = 4.01

Molecular weight: 10.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514U0X0|A0A514U0X0_9CAUD Uncharacterized protein OS=Gordonia phage Phendrix OX=2593335 GN=39 PE=4 SV=1
MM1 pKa = 7.21ITTLFNTVKK10 pKa = 9.72VLHH13 pKa = 6.38SGRR16 pKa = 11.84VFEE19 pKa = 4.73YY20 pKa = 9.9PVEE23 pKa = 4.38TVTGAQMRR31 pKa = 11.84RR32 pKa = 11.84YY33 pKa = 7.04TAKK36 pKa = 10.16CRR38 pKa = 11.84CGWEE42 pKa = 4.22SAPVTTASSAKK53 pKa = 9.5QRR55 pKa = 11.84AHH57 pKa = 5.13RR58 pKa = 11.84HH59 pKa = 5.35MITVWEE65 pKa = 4.07QRR67 pKa = 11.84TQVEE71 pKa = 4.41MRR73 pKa = 3.75

Molecular weight: 8.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

188

0

188

34352

29

2221

182.7

20.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.03 ± 0.41

1.202 ± 0.117

6.937 ± 0.219

6.535 ± 0.216

3.036 ± 0.095

7.051 ± 0.257

2.46 ± 0.147

4.955 ± 0.189

5.371 ± 0.302

7.24 ± 0.178

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.803 ± 0.106

3.63 ± 0.174

4.792 ± 0.172

3.051 ± 0.127

6.457 ± 0.215

6.11 ± 0.219

6.608 ± 0.231

7.272 ± 0.218

2.154 ± 0.097

3.307 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski