Clostridium gasigenes

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3849 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H0UT61|A0A1H0UT61_9CLOT Pyruvate-ferredoxin/flavodoxin oxidoreductase OS=Clostridium gasigenes OX=94869 GN=SAMN04488529_11263 PE=3 SV=1
MM1 pKa = 7.39SKK3 pKa = 10.28EE4 pKa = 4.04LNKK7 pKa = 10.68CCSTEE12 pKa = 3.95GNEE15 pKa = 4.89GCGCSHH21 pKa = 7.11DD22 pKa = 4.35HH23 pKa = 6.92EE24 pKa = 4.46EE25 pKa = 5.35HH26 pKa = 6.75KK27 pKa = 11.24GCGDD31 pKa = 3.35NCGCGHH37 pKa = 6.19DD38 pKa = 4.78HH39 pKa = 6.29EE40 pKa = 6.75AEE42 pKa = 4.22HH43 pKa = 6.67EE44 pKa = 4.6GEE46 pKa = 4.84CGDD49 pKa = 4.45DD50 pKa = 4.04CGCGDD55 pKa = 4.21HH56 pKa = 7.21EE57 pKa = 5.21DD58 pKa = 3.81FVVDD62 pKa = 5.8LEE64 pKa = 4.65NEE66 pKa = 4.2DD67 pKa = 3.96GTVVSCPIIDD77 pKa = 3.3AFEE80 pKa = 4.28FEE82 pKa = 4.34EE83 pKa = 4.35NEE85 pKa = 4.28YY86 pKa = 10.66ILAQNPEE93 pKa = 4.19EE94 pKa = 4.36DD95 pKa = 3.2SVYY98 pKa = 10.73LFRR101 pKa = 11.84STEE104 pKa = 4.0SGEE107 pKa = 4.51LEE109 pKa = 4.25VPDD112 pKa = 3.78EE113 pKa = 4.4VEE115 pKa = 3.85FDD117 pKa = 3.52RR118 pKa = 11.84VSAYY122 pKa = 10.51YY123 pKa = 10.33SEE125 pKa = 5.2LADD128 pKa = 4.07EE129 pKa = 4.62SQEE132 pKa = 4.12SS133 pKa = 3.51

Molecular weight: 14.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H0Q4W7|A0A1H0Q4W7_9CLOT Glutathione peroxidase OS=Clostridium gasigenes OX=94869 GN=H7E67_07530 PE=3 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84KK3 pKa = 9.57KK4 pKa = 10.71LIIINGVMGVGKK16 pKa = 7.0TTISRR21 pKa = 11.84ALLVKK26 pKa = 10.27RR27 pKa = 11.84NKK29 pKa = 9.81IFQRR33 pKa = 11.84KK34 pKa = 7.16

Molecular weight: 3.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3849

0

3849

1144006

24

2845

297.2

33.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.417 ± 0.043

1.161 ± 0.016

5.443 ± 0.03

7.641 ± 0.047

4.306 ± 0.031

6.55 ± 0.038

1.242 ± 0.014

10.348 ± 0.057

9.197 ± 0.04

9.024 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.633 ± 0.02

6.471 ± 0.042

2.622 ± 0.025

2.152 ± 0.02

3.203 ± 0.025

6.316 ± 0.029

5.024 ± 0.029

6.492 ± 0.033

0.675 ± 0.011

4.081 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski