Mycobacterium phage SpongeBob

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nclasvirinae; Charlievirus; unclassified Charlievirus

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481VVU5|A0A481VVU5_9CAUD Excise OS=Mycobacterium phage SpongeBob OX=2530151 GN=35 PE=4 SV=1
MM1 pKa = 6.56TTPMTLNLDD10 pKa = 3.26QPMFVPCGTQVITTQPLVVEE30 pKa = 4.5LADD33 pKa = 3.63YY34 pKa = 10.68DD35 pKa = 4.04YY36 pKa = 11.58TPFADD41 pKa = 4.08PLAPAFDD48 pKa = 4.38FLADD52 pKa = 3.9RR53 pKa = 11.84LHH55 pKa = 7.5PDD57 pKa = 2.94GTLDD61 pKa = 4.12RR62 pKa = 11.84DD63 pKa = 4.36VPCTSLPGRR72 pKa = 11.84STIEE76 pKa = 4.24RR77 pKa = 11.84ITT79 pKa = 3.33

Molecular weight: 8.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481VWG9|A0A481VWG9_9CAUD Minor tail protein OS=Mycobacterium phage SpongeBob OX=2530151 GN=18 PE=4 SV=1
MM1 pKa = 7.28HH2 pKa = 7.5VIPANFGGPMRR13 pKa = 11.84RR14 pKa = 11.84EE15 pKa = 4.24CAVCGQPFEE24 pKa = 4.28AQRR27 pKa = 11.84PQAKK31 pKa = 9.99YY32 pKa = 10.5CGEE35 pKa = 4.18TCRR38 pKa = 11.84KK39 pKa = 8.35RR40 pKa = 11.84AQRR43 pKa = 11.84GGIAKK48 pKa = 9.84QKK50 pKa = 8.75QQQTAPAPAASAAPSGGGLIEE71 pKa = 4.24TVQAALEE78 pKa = 4.07QADD81 pKa = 4.59RR82 pKa = 11.84LNTIAGQHH90 pKa = 5.55ALEE93 pKa = 4.05LARR96 pKa = 11.84RR97 pKa = 11.84IVYY100 pKa = 10.17APGMNTGVAALSKK113 pKa = 10.36QLQAVLAEE121 pKa = 4.45ALAGTAPVAADD132 pKa = 3.61PVDD135 pKa = 3.63EE136 pKa = 4.52LKK138 pKa = 10.88ARR140 pKa = 11.84RR141 pKa = 11.84DD142 pKa = 3.29AKK144 pKa = 10.34RR145 pKa = 11.84RR146 pKa = 11.84RR147 pKa = 11.84GAA149 pKa = 3.22

Molecular weight: 15.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

13372

38

1033

205.7

22.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.287 ± 0.71

1.144 ± 0.205

6.671 ± 0.26

5.923 ± 0.302

2.902 ± 0.18

8.712 ± 0.54

1.952 ± 0.162

4.554 ± 0.179

3.006 ± 0.209

7.8 ± 0.236

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.989 ± 0.162

3.059 ± 0.212

5.968 ± 0.23

3.769 ± 0.243

6.858 ± 0.481

5.078 ± 0.195

6.73 ± 0.263

7.389 ± 0.337

1.877 ± 0.175

2.333 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski