Spirosoma endophyticum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Spirosoma

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7570 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I2BT17|A0A1I2BT17_9BACT Transposase (Fragment) OS=Spirosoma endophyticum OX=662367 GN=SAMN05216167_1161 PE=4 SV=1
MM1 pKa = 7.73EE2 pKa = 5.39EE3 pKa = 4.15YY4 pKa = 10.26FIKK7 pKa = 10.02IDD9 pKa = 4.29PYY11 pKa = 11.36SEE13 pKa = 4.58TIDD16 pKa = 3.45QIIRR20 pKa = 11.84SDD22 pKa = 3.37SYY24 pKa = 11.98AYY26 pKa = 10.32LAHH29 pKa = 6.64QLGCRR34 pKa = 11.84HH35 pKa = 5.71MGIYY39 pKa = 10.37AKK41 pKa = 10.45QPFLDD46 pKa = 3.5AAMMNAEE53 pKa = 4.18ALEE56 pKa = 4.3TDD58 pKa = 4.89LLPAAWRR65 pKa = 11.84YY66 pKa = 9.91LPTGEE71 pKa = 4.95VYY73 pKa = 10.76FGPAVWLNISEE84 pKa = 4.18NGEE87 pKa = 3.73LRR89 pKa = 11.84VPLRR93 pKa = 11.84RR94 pKa = 11.84IDD96 pKa = 3.59QLEE99 pKa = 4.04DD100 pKa = 3.69EE101 pKa = 4.79IEE103 pKa = 3.88FLGFIEE109 pKa = 4.85ACTDD113 pKa = 4.12FLPLGEE119 pKa = 4.74NLLDD123 pKa = 4.68DD124 pKa = 4.66SEE126 pKa = 6.1CGDD129 pKa = 3.78EE130 pKa = 4.0QSPDD134 pKa = 3.02IEE136 pKa = 4.43FF137 pKa = 4.15

Molecular weight: 15.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I2HN69|A0A1I2HN69_9BACT Resolvase N terminal domain OS=Spirosoma endophyticum OX=662367 GN=SAMN05216167_1467 PE=4 SV=1
MM1 pKa = 7.79KK2 pKa = 10.6NRR4 pKa = 11.84ILVGAFLLAITSLTTQAQSTASGSSNTGTPTSGATPAQNGSTGATVSGTKK54 pKa = 10.15AGSGTMSQGGSKK66 pKa = 10.48SSGRR70 pKa = 11.84KK71 pKa = 7.13MKK73 pKa = 9.74RR74 pKa = 11.84TSKK77 pKa = 10.45ASKK80 pKa = 10.13SSSLSSTGRR89 pKa = 3.23

Molecular weight: 8.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7570

0

7570

2451505

29

2528

323.8

36.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.1 ± 0.026

0.717 ± 0.009

5.327 ± 0.019

5.083 ± 0.03

4.481 ± 0.017

7.113 ± 0.028

1.868 ± 0.015

6.022 ± 0.024

5.264 ± 0.026

10.119 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.156 ± 0.012

4.73 ± 0.028

4.561 ± 0.018

4.489 ± 0.019

5.088 ± 0.024

6.359 ± 0.024

6.546 ± 0.028

6.801 ± 0.02

1.322 ± 0.012

3.853 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski