Brachybacterium alimentarium

Taxonomy:

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3588 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A3YI87|A0A2A3YI87_9MICO Transcriptional regulator OS=Brachybacterium alimentarium OX=47845 GN=CIK66_11755 PE=4 SV=1
MM1 pKa = 7.27AQPRR5 pKa = 11.84SRR7 pKa = 11.84TPLIVGIFAAIACLALVLLVVAALIAGIVYY37 pKa = 9.56FRR39 pKa = 11.84SLDD42 pKa = 3.54SGGSGGSGDD51 pKa = 3.83QRR53 pKa = 11.84DD54 pKa = 4.11GTSTSGEE61 pKa = 4.71LITPPGVDD69 pKa = 3.0EE70 pKa = 4.56DD71 pKa = 3.91QPYY74 pKa = 11.1LEE76 pKa = 5.62LSTSSDD82 pKa = 3.57GPVVDD87 pKa = 4.73VYY89 pKa = 11.78LDD91 pKa = 3.78FLCPHH96 pKa = 7.06CKK98 pKa = 9.36TFEE101 pKa = 3.99EE102 pKa = 4.58AQGEE106 pKa = 4.32DD107 pKa = 3.76LAQLALDD114 pKa = 3.98GEE116 pKa = 4.78ITLRR120 pKa = 11.84MHH122 pKa = 7.19PRR124 pKa = 11.84PMLDD128 pKa = 3.3PNSTPVGYY136 pKa = 10.11SGRR139 pKa = 11.84AANAAVCAYY148 pKa = 10.53AEE150 pKa = 4.64DD151 pKa = 4.5PEE153 pKa = 4.59LWFPAEE159 pKa = 4.01SALFEE164 pKa = 4.37AQPGTEE170 pKa = 4.06GLTDD174 pKa = 4.01DD175 pKa = 5.06EE176 pKa = 4.66LAQVVDD182 pKa = 4.0EE183 pKa = 4.41ATGLDD188 pKa = 3.51VGQCIAEE195 pKa = 4.28GTYY198 pKa = 10.58LPWIEE203 pKa = 5.03DD204 pKa = 3.47VVEE207 pKa = 4.42PEE209 pKa = 4.9AGSSTQGTPTVLLDD223 pKa = 3.55GEE225 pKa = 4.57QFTGDD230 pKa = 3.47LAAPGNVKK238 pKa = 10.16EE239 pKa = 4.38AVEE242 pKa = 4.06AAA244 pKa = 3.57

Molecular weight: 25.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A3YM08|A0A2A3YM08_9MICO Bifunctional purine biosynthesis protein PurH OS=Brachybacterium alimentarium OX=47845 GN=purH PE=3 SV=1
MM1 pKa = 7.28GSVVKK6 pKa = 10.44KK7 pKa = 9.48RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.32RR11 pKa = 11.84MSKK14 pKa = 9.94KK15 pKa = 9.5KK16 pKa = 9.79HH17 pKa = 5.45RR18 pKa = 11.84KK19 pKa = 6.15QLRR22 pKa = 11.84KK23 pKa = 7.92TRR25 pKa = 11.84HH26 pKa = 3.43QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3588

0

3588

1233535

32

3486

343.8

36.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.835 ± 0.052

0.545 ± 0.009

6.437 ± 0.035

6.211 ± 0.039

2.821 ± 0.029

9.064 ± 0.035

2.273 ± 0.019

4.215 ± 0.032

1.698 ± 0.025

10.465 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.984 ± 0.017

1.691 ± 0.017

5.646 ± 0.031

2.981 ± 0.019

7.413 ± 0.046

5.965 ± 0.025

6.139 ± 0.025

8.337 ± 0.043

1.442 ± 0.018

1.838 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski