Mycobacterium phage Kimona

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A249XU38|A0A249XU38_9CAUD Uncharacterized protein OS=Mycobacterium phage Kimona OX=2024295 GN=77 PE=4 SV=1
MM1 pKa = 7.76PEE3 pKa = 4.64GYY5 pKa = 8.47TEE7 pKa = 4.68AEE9 pKa = 3.79WLGFKK14 pKa = 10.32PGAPTLPYY22 pKa = 10.0RR23 pKa = 11.84EE24 pKa = 4.3EE25 pKa = 4.26PDD27 pKa = 3.11YY28 pKa = 11.6SLIHH32 pKa = 6.87DD33 pKa = 4.47YY34 pKa = 10.97VWEE37 pKa = 4.72GDD39 pKa = 3.62ADD41 pKa = 4.09GEE43 pKa = 4.38

Molecular weight: 4.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A249XU23|A0A249XU23_9CAUD Uncharacterized protein OS=Mycobacterium phage Kimona OX=2024295 GN=35 PE=4 SV=1
MM1 pKa = 7.81LSIQQTADD9 pKa = 3.07HH10 pKa = 6.78LGVSTKK16 pKa = 7.21TVRR19 pKa = 11.84RR20 pKa = 11.84YY21 pKa = 9.82IADD24 pKa = 3.35GRR26 pKa = 11.84IKK28 pKa = 10.45AVRR31 pKa = 11.84LGPRR35 pKa = 11.84LIRR38 pKa = 11.84VDD40 pKa = 3.36RR41 pKa = 11.84SSVEE45 pKa = 3.63EE46 pKa = 3.82LMRR49 pKa = 11.84PIGNYY54 pKa = 9.53AA55 pKa = 3.13

Molecular weight: 6.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

15512

23

1011

178.3

19.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.128 ± 0.446

0.845 ± 0.132

6.215 ± 0.273

6.924 ± 0.396

3.262 ± 0.256

8.297 ± 0.372

2.256 ± 0.223

4.558 ± 0.145

4.422 ± 0.195

8.297 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.501 ± 0.138

3.236 ± 0.183

5.299 ± 0.286

3.823 ± 0.341

7.265 ± 0.454

4.874 ± 0.215

5.628 ± 0.262

7.156 ± 0.201

2.044 ± 0.145

2.972 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski