Rubripirellula amarantea

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Rubripirellula

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5179 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C5WU88|A0A5C5WU88_9BACT NAD_binding_9 domain-containing protein OS=Rubripirellula amarantea OX=2527999 GN=Pla22_14220 PE=4 SV=1
MM1 pKa = 7.03NRR3 pKa = 11.84SYY5 pKa = 11.17LCKK8 pKa = 10.1RR9 pKa = 11.84SQGFFGLWSIAIALAAMPPDD29 pKa = 3.43QSHH32 pKa = 6.29AQTIVATNPPTQITTEE48 pKa = 4.0TFYY51 pKa = 10.93EE52 pKa = 3.75IDD54 pKa = 3.42VAYY57 pKa = 10.69SIADD61 pKa = 3.56DD62 pKa = 5.76AIVQVQLFQMLEE74 pKa = 4.02NSWAFIDD81 pKa = 4.4QVWEE85 pKa = 4.02PVLDD89 pKa = 4.16GSTSIAIPFYY99 pKa = 10.61VPEE102 pKa = 4.1EE103 pKa = 3.96AAPEE107 pKa = 4.14DD108 pKa = 3.73NYY110 pKa = 11.06LWQVILYY117 pKa = 9.9NGSDD121 pKa = 3.34WEE123 pKa = 4.36KK124 pKa = 10.31LDD126 pKa = 4.38EE127 pKa = 4.84EE128 pKa = 4.25IFYY131 pKa = 9.85PITVSANAPDD141 pKa = 4.43VITGSNAPPEE151 pKa = 4.34VQTNEE156 pKa = 4.28SYY158 pKa = 11.33DD159 pKa = 3.38VTVEE163 pKa = 3.93YY164 pKa = 10.18EE165 pKa = 3.77ISVDD169 pKa = 4.68GIVQVQLLDD178 pKa = 3.89SSWNVIDD185 pKa = 4.91QDD187 pKa = 3.57WTSVDD192 pKa = 3.68SGSEE196 pKa = 4.13SATVTIDD203 pKa = 3.53VPIDD207 pKa = 3.28QPLADD212 pKa = 4.22DD213 pKa = 5.16SIWQIMLYY221 pKa = 10.2RR222 pKa = 11.84QDD224 pKa = 3.7PNTWTKK230 pKa = 10.68LDD232 pKa = 4.12EE233 pKa = 5.27IIAEE237 pKa = 4.96GIAIVGNMGGPNLEE251 pKa = 4.12WLPPGNSWALEE262 pKa = 3.99WQDD265 pKa = 3.47EE266 pKa = 4.55FEE268 pKa = 4.52GTGLPEE274 pKa = 3.11NWYY277 pKa = 9.0PHH279 pKa = 6.93LGTNPEE285 pKa = 4.12EE286 pKa = 4.29VSNEE290 pKa = 3.38IAAAAAEE297 pKa = 4.08NRR299 pKa = 11.84IPEE302 pKa = 4.07YY303 pKa = 9.44PLRR306 pKa = 11.84YY307 pKa = 9.68GPYY310 pKa = 9.62DD311 pKa = 3.65GNNAWMFSTGYY322 pKa = 10.03GNHH325 pKa = 6.74WLDD328 pKa = 4.02GSGNLQLQIRR338 pKa = 11.84ADD340 pKa = 3.92LNVDD344 pKa = 4.12LAHH347 pKa = 6.31GKK349 pKa = 9.65KK350 pKa = 9.92VEE352 pKa = 4.07SAYY355 pKa = 11.11LMSGQPVAWDD365 pKa = 3.62DD366 pKa = 3.99SVPGTGVRR374 pKa = 11.84WEE376 pKa = 4.29GTLVSPGDD384 pKa = 3.59GEE386 pKa = 4.76IYY388 pKa = 10.15ISTRR392 pKa = 11.84VKK394 pKa = 8.14TNEE397 pKa = 3.63MVGYY401 pKa = 7.57STWFAFWLFTQTQAYY416 pKa = 9.92DD417 pKa = 3.51RR418 pKa = 11.84VPATGTEE425 pKa = 3.55IDD427 pKa = 4.34IIEE430 pKa = 4.25IPIGEE435 pKa = 4.62PAWMDD440 pKa = 3.28GVFNVAHH447 pKa = 6.52HH448 pKa = 6.61WGVPTQAEE456 pKa = 4.4LNAGIKK462 pKa = 10.35FSEE465 pKa = 4.19SLQYY469 pKa = 10.88SGVIAEE475 pKa = 4.52SLADD479 pKa = 3.65VTADD483 pKa = 3.38EE484 pKa = 4.36YY485 pKa = 10.67HH486 pKa = 6.4TYY488 pKa = 9.96GLRR491 pKa = 11.84WSADD495 pKa = 2.91SMTTYY500 pKa = 11.08VNGVEE505 pKa = 4.42TFTFPDD511 pKa = 3.94NPPGNPVPAIPANPSDD527 pKa = 3.94MMMMLTLEE535 pKa = 4.28FEE537 pKa = 3.86KK538 pKa = 10.98DD539 pKa = 2.78AWVDD543 pKa = 3.37KK544 pKa = 10.73TLDD547 pKa = 3.59DD548 pKa = 3.87QGDD551 pKa = 3.63GRR553 pKa = 11.84FQGTPLEE560 pKa = 4.14EE561 pKa = 4.28DD562 pKa = 2.79ANYY565 pKa = 10.82RR566 pKa = 11.84VMSRR570 pKa = 11.84AQVDD574 pKa = 3.66YY575 pKa = 10.93VRR577 pKa = 11.84IWRR580 pKa = 11.84AQQ582 pKa = 2.99

Molecular weight: 64.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C5WII3|A0A5C5WII3_9BACT PEP-CTERM motif protein OS=Rubripirellula amarantea OX=2527999 GN=Pla22_48190 PE=4 SV=1
MM1 pKa = 6.89TLAVAMTFAVPMKK14 pKa = 8.98FTVPMKK20 pKa = 9.32FTVPMTFTVPMTFTVPGPRR39 pKa = 11.84NQRR42 pKa = 11.84AQPRR46 pKa = 11.84GRR48 pKa = 11.84RR49 pKa = 11.84SKK51 pKa = 9.64PARR54 pKa = 11.84PVVTTARR61 pKa = 11.84AAGAA65 pKa = 3.57

Molecular weight: 7.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5179

0

5179

2058698

29

14289

397.5

43.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.376 ± 0.037

1.054 ± 0.019

6.661 ± 0.056

5.925 ± 0.032

3.727 ± 0.019

7.722 ± 0.063

2.132 ± 0.027

5.349 ± 0.022

3.534 ± 0.037

9.37 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.327 ± 0.028

3.461 ± 0.033

4.877 ± 0.031

3.893 ± 0.024

6.247 ± 0.051

7.283 ± 0.036

5.862 ± 0.057

7.44 ± 0.029

1.39 ± 0.017

2.368 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski