Dolosigranulum pigrum ATCC 51524

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Carnobacteriaceae; Dolosigranulum; Dolosigranulum pigrum

Average proteome isoelectric point is 5.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1691 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H3NFW1|H3NFW1_9LACT Uncharacterized protein OS=Dolosigranulum pigrum ATCC 51524 OX=883103 GN=HMPREF9703_01442 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.28KK3 pKa = 10.67YY4 pKa = 10.54MFMALFLGLSGFLMGCHH21 pKa = 6.18YY22 pKa = 10.91LDD24 pKa = 3.85SQDD27 pKa = 3.34RR28 pKa = 11.84SAMGSVQTGQNTDD41 pKa = 3.3PAGEE45 pKa = 4.17IPRR48 pKa = 11.84ADD50 pKa = 4.14DD51 pKa = 3.86LPLGEE56 pKa = 5.66LYY58 pKa = 10.22IEE60 pKa = 4.58INDD63 pKa = 4.21NIPVFDD69 pKa = 4.2SEE71 pKa = 4.43LLEE74 pKa = 4.71AEE76 pKa = 4.36EE77 pKa = 4.56PFYY80 pKa = 11.25DD81 pKa = 5.71LSPLDD86 pKa = 3.43QFGRR90 pKa = 11.84AQAAIALLDD99 pKa = 3.94EE100 pKa = 4.77EE101 pKa = 5.25LLPPEE106 pKa = 4.12QRR108 pKa = 11.84GDD110 pKa = 3.44NPTFEE115 pKa = 4.33PSGWKK120 pKa = 7.95QANYY124 pKa = 10.37EE125 pKa = 4.12IVSGNWLYY133 pKa = 11.18NRR135 pKa = 11.84SHH137 pKa = 6.78LLGWQLTGNNDD148 pKa = 3.39YY149 pKa = 11.46EE150 pKa = 4.3NFITGTRR157 pKa = 11.84QFNAEE162 pKa = 3.54GMLPFEE168 pKa = 4.55NFVAHH173 pKa = 6.19VVEE176 pKa = 3.86QGMRR180 pKa = 11.84VVYY183 pKa = 10.04SVIPIYY189 pKa = 11.22DD190 pKa = 3.58NDD192 pKa = 3.6NLVPHH197 pKa = 7.03GVQMMGWSQDD207 pKa = 3.76DD208 pKa = 3.57NGEE211 pKa = 4.16TLQFNIFVPNEE222 pKa = 3.81QAGVMIDD229 pKa = 3.7YY230 pKa = 10.88SDD232 pKa = 3.63GSSYY236 pKa = 11.49LEE238 pKa = 3.95

Molecular weight: 26.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H3NDX5|H3NDX5_9LACT Isoleucine--tRNA ligase OS=Dolosigranulum pigrum ATCC 51524 OX=883103 GN=ileS PE=3 SV=1
MM1 pKa = 7.62AVPKK5 pKa = 10.35RR6 pKa = 11.84RR7 pKa = 11.84TSTTRR12 pKa = 11.84KK13 pKa = 8.72RR14 pKa = 11.84KK15 pKa = 9.52RR16 pKa = 11.84RR17 pKa = 11.84THH19 pKa = 6.69LGLKK23 pKa = 9.02LPNMNEE29 pKa = 3.84CRR31 pKa = 11.84NCGEE35 pKa = 4.52MKK37 pKa = 10.21RR38 pKa = 11.84NHH40 pKa = 6.41HH41 pKa = 6.0VCPSCGHH48 pKa = 5.97YY49 pKa = 10.66GGEE52 pKa = 4.14QVIAKK57 pKa = 9.48NAA59 pKa = 3.48

Molecular weight: 6.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1691

0

1691

537359

32

2443

317.8

35.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.323 ± 0.07

0.504 ± 0.015

6.089 ± 0.053

7.703 ± 0.06

4.131 ± 0.047

6.641 ± 0.055

2.171 ± 0.032

7.666 ± 0.071

5.717 ± 0.061

9.698 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.613 ± 0.033

4.349 ± 0.049

3.449 ± 0.037

4.588 ± 0.051

4.132 ± 0.043

5.891 ± 0.037

5.826 ± 0.043

6.968 ± 0.047

0.847 ± 0.018

3.694 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski