Flavobacterium indicum (strain DSM 17447 / CIP 109464 / GPTSA100-9)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; Flavobacterium indicum

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2649 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H8XUA0|H8XUA0_FLAIG DNA recombination protein RmuC OS=Flavobacterium indicum (strain DSM 17447 / CIP 109464 / GPTSA100-9) OX=1094466 GN=rmuC PE=3 SV=1
MM1 pKa = 7.75PNYY4 pKa = 10.16IIDD7 pKa = 4.14LSDD10 pKa = 3.57AEE12 pKa = 4.2SWGRR16 pKa = 11.84SWQTAPPKK24 pKa = 10.54DD25 pKa = 3.55LAKK28 pKa = 10.57AHH30 pKa = 5.91QIPLEE35 pKa = 3.97VLNGLIEE42 pKa = 4.45TPDD45 pKa = 3.38MASIRR50 pKa = 11.84AYY52 pKa = 10.06MGVDD56 pKa = 2.55SGGIQRR62 pKa = 11.84LMIVAVDD69 pKa = 3.75SNGNDD74 pKa = 4.91LIDD77 pKa = 3.91NNNNQFIYY85 pKa = 10.39SGTSPCPEE93 pKa = 4.06NCDD96 pKa = 3.42TSSPLYY102 pKa = 10.74NPP104 pKa = 4.4

Molecular weight: 11.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H8XUA1|H8XUA1_FLAIG Pseudouridine synthase OS=Flavobacterium indicum (strain DSM 17447 / CIP 109464 / GPTSA100-9) OX=1094466 GN=KQS_04555 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.91HH17 pKa = 4.39GFMDD21 pKa = 4.5RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.41KK42 pKa = 10.14LTVSSEE48 pKa = 3.92PRR50 pKa = 11.84HH51 pKa = 5.77KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2649

0

2649

890273

38

2372

336.1

37.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.236 ± 0.053

0.835 ± 0.018

4.971 ± 0.036

6.5 ± 0.061

5.52 ± 0.046

6.157 ± 0.059

1.677 ± 0.022

8.184 ± 0.049

8.176 ± 0.086

9.086 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.089 ± 0.029

6.817 ± 0.061

3.256 ± 0.029

3.564 ± 0.03

2.909 ± 0.03

6.375 ± 0.047

6.17 ± 0.096

6.298 ± 0.042

0.98 ± 0.017

4.199 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski