Pseudomonas bauzanensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3223 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A031ME59|A0A031ME59_9PSED Uncharacterized protein OS=Pseudomonas bauzanensis OX=653930 GN=SAMN04487855_1747 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 10.04KK3 pKa = 8.44WQCIVCGFIYY13 pKa = 10.49DD14 pKa = 4.14EE15 pKa = 4.74AEE17 pKa = 3.72GWPDD21 pKa = 4.01DD22 pKa = 4.65GIAPGTRR29 pKa = 11.84WEE31 pKa = 4.84DD32 pKa = 3.65VPADD36 pKa = 3.99WQCPDD41 pKa = 3.85CGVGKK46 pKa = 10.53EE47 pKa = 4.19DD48 pKa = 4.87FEE50 pKa = 4.83MIAIGG55 pKa = 4.11

Molecular weight: 6.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4LRW3|A0A1I4LRW3_9PSED Ribonuclease P protein component OS=Pseudomonas bauzanensis OX=653930 GN=rnpA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.29GRR39 pKa = 11.84HH40 pKa = 5.49KK41 pKa = 10.9LSAA44 pKa = 3.8

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3223

0

3223

1068713

23

2081

331.6

36.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.658 ± 0.046

0.995 ± 0.015

5.594 ± 0.036

6.034 ± 0.036

3.542 ± 0.03

7.931 ± 0.04

2.31 ± 0.019

5.078 ± 0.033

2.951 ± 0.032

11.633 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.557 ± 0.017

3.018 ± 0.025

4.878 ± 0.026

4.693 ± 0.036

6.687 ± 0.037

5.609 ± 0.028

4.925 ± 0.024

6.99 ± 0.033

1.436 ± 0.018

2.482 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski