Chimeric virus 14

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5ALV7|A0A0C5ALV7_9VIRU Putative replication protein OS=Chimeric virus 14 OX=1608440 GN=rep PE=3 SV=1
MM1 pKa = 7.44SAPWDD6 pKa = 3.84NDD8 pKa = 3.2SEE10 pKa = 4.51EE11 pKa = 4.17YY12 pKa = 11.02SNEE15 pKa = 3.83EE16 pKa = 4.33KK17 pKa = 9.97IDD19 pKa = 3.48RR20 pKa = 11.84APNRR24 pKa = 11.84KK25 pKa = 8.95VKK27 pKa = 10.42IKK29 pKa = 10.3IAEE32 pKa = 4.21DD33 pKa = 4.0PYY35 pKa = 11.24EE36 pKa = 4.39EE37 pKa = 4.59PSEE40 pKa = 4.52DD41 pKa = 5.67GIIQDD46 pKa = 3.88LDD48 pKa = 3.74EE49 pKa = 6.32KK50 pKa = 11.31EE51 pKa = 4.5EE52 pKa = 3.98EE53 pKa = 4.3LDD55 pKa = 3.79NFSKK59 pKa = 10.86SALRR63 pKa = 11.84SNGVFKK69 pKa = 10.9INNRR73 pKa = 11.84QVMLTYY79 pKa = 8.94KK80 pKa = 9.2THH82 pKa = 7.23LDD84 pKa = 3.46KK85 pKa = 11.17EE86 pKa = 4.72DD87 pKa = 3.32YY88 pKa = 10.6RR89 pKa = 11.84SFVDD93 pKa = 3.74EE94 pKa = 4.67LAPFDD99 pKa = 4.2EE100 pKa = 5.56LFIAHH105 pKa = 6.77EE106 pKa = 4.24NADD109 pKa = 3.61LKK111 pKa = 11.28NPYY114 pKa = 9.39KK115 pKa = 9.71HH116 pKa = 4.63THH118 pKa = 6.09AYY120 pKa = 9.13IKK122 pKa = 9.22FTIPFVSSDD131 pKa = 3.1SRR133 pKa = 11.84VFDD136 pKa = 3.44WDD138 pKa = 3.3VSEE141 pKa = 4.31TQNIHH146 pKa = 4.9PHH148 pKa = 5.93ISVLGAKK155 pKa = 9.83KK156 pKa = 10.23VDD158 pKa = 4.24EE159 pKa = 4.79IKK161 pKa = 10.07MKK163 pKa = 10.84YY164 pKa = 9.94YY165 pKa = 9.95ISKK168 pKa = 9.96EE169 pKa = 4.09DD170 pKa = 3.71KK171 pKa = 11.19SPDD174 pKa = 3.28LAMLRR179 pKa = 11.84VSCDD183 pKa = 2.87DD184 pKa = 3.33WNKK187 pKa = 10.85ANGNKK192 pKa = 9.45ARR194 pKa = 11.84LGKK197 pKa = 10.51GLADD201 pKa = 3.91DD202 pKa = 3.76QVSLLEE208 pKa = 4.25RR209 pKa = 11.84VCSYY213 pKa = 9.39KK214 pKa = 10.34TYY216 pKa = 11.01QEE218 pKa = 4.11MLKK221 pKa = 10.08HH222 pKa = 5.55LCKK225 pKa = 10.57EE226 pKa = 4.24PGHH229 pKa = 5.61ATGLRR234 pKa = 11.84EE235 pKa = 3.9LWEE238 pKa = 4.41SKK240 pKa = 10.76LIAEE244 pKa = 4.36KK245 pKa = 10.59PKK247 pKa = 10.56IKK249 pKa = 10.29FSEE252 pKa = 4.09IAEE255 pKa = 4.3FTWQKK260 pKa = 8.73TMRR263 pKa = 11.84DD264 pKa = 3.4KK265 pKa = 11.4LLAPEE270 pKa = 4.09WNHH273 pKa = 7.38RR274 pKa = 11.84ILTYY278 pKa = 10.39IVDD281 pKa = 3.87KK282 pKa = 11.07KK283 pKa = 10.95GGSGKK288 pKa = 8.56STFADD293 pKa = 3.41YY294 pKa = 10.05MVNTYY299 pKa = 10.23NALYY303 pKa = 9.66LSSVTSSRR311 pKa = 11.84DD312 pKa = 2.88IATLIDD318 pKa = 3.44EE319 pKa = 4.53HH320 pKa = 6.76LKK322 pKa = 11.06CGGSAKK328 pKa = 10.57YY329 pKa = 10.11IILDD333 pKa = 3.76LPRR336 pKa = 11.84ASKK339 pKa = 9.51AHH341 pKa = 6.07KK342 pKa = 9.51MWDD345 pKa = 3.4TVEE348 pKa = 4.63ALLNGKK354 pKa = 8.24LTVGKK359 pKa = 9.35WKK361 pKa = 10.55GKK363 pKa = 8.75PLHH366 pKa = 6.37FDD368 pKa = 3.27KK369 pKa = 10.95PRR371 pKa = 11.84LIVFANFAPPLPGDD385 pKa = 3.66EE386 pKa = 4.34SRR388 pKa = 11.84AADD391 pKa = 3.63VCEE394 pKa = 4.51DD395 pKa = 3.76NCVLSPDD402 pKa = 3.9RR403 pKa = 11.84WDD405 pKa = 3.16IWDD408 pKa = 4.06IVCLDD413 pKa = 3.46AAKK416 pKa = 10.69AHH418 pKa = 6.68DD419 pKa = 4.45GEE421 pKa = 4.74HH422 pKa = 6.5DD423 pKa = 4.04RR424 pKa = 11.84IMVNRR429 pKa = 11.84ANPHH433 pKa = 6.79RR434 pKa = 11.84DD435 pKa = 3.69LNWVKK440 pKa = 10.47PKK442 pKa = 10.53RR443 pKa = 11.84FQNLDD448 pKa = 3.44YY449 pKa = 11.43

Molecular weight: 51.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5ALV7|A0A0C5ALV7_9VIRU Putative replication protein OS=Chimeric virus 14 OX=1608440 GN=rep PE=3 SV=1
MM1 pKa = 6.64STRR4 pKa = 11.84RR5 pKa = 11.84YY6 pKa = 8.75VKK8 pKa = 10.11KK9 pKa = 10.7SKK11 pKa = 10.86APAPKK16 pKa = 8.99IVYY19 pKa = 9.06VAAPPVAKK27 pKa = 10.0RR28 pKa = 11.84SYY30 pKa = 8.18TRR32 pKa = 11.84KK33 pKa = 10.01SSTAGGLRR41 pKa = 11.84GHH43 pKa = 7.33GDD45 pKa = 3.31YY46 pKa = 10.62TYY48 pKa = 10.86DD49 pKa = 5.25KK50 pKa = 9.69PGPWGNFGRR59 pKa = 11.84SVGKK63 pKa = 9.61QAGAMLGSNYY73 pKa = 10.55GLGKK77 pKa = 10.52AGGAIGSKK85 pKa = 10.28LGGYY89 pKa = 8.35LHH91 pKa = 6.98YY92 pKa = 10.02IGKK95 pKa = 9.43IFGSGDD101 pKa = 3.66YY102 pKa = 8.85VTSSSQVRR110 pKa = 11.84NNILVNQSQVPQFQDD125 pKa = 2.48GKK127 pKa = 7.84NTVRR131 pKa = 11.84IAHH134 pKa = 6.67RR135 pKa = 11.84EE136 pKa = 3.83YY137 pKa = 10.83LGDD140 pKa = 4.72IITSSTPGAFSIEE153 pKa = 4.42SYY155 pKa = 10.09PINPGVSGSFPWLANVVGASFQQYY179 pKa = 9.58RR180 pKa = 11.84INGMVFEE187 pKa = 4.57FRR189 pKa = 11.84SMSADD194 pKa = 2.99ALTSTNTALGTVAMATDD211 pKa = 4.31YY212 pKa = 11.27DD213 pKa = 4.2SKK215 pKa = 11.26DD216 pKa = 3.16TAFTSKK222 pKa = 10.01QQMEE226 pKa = 4.15NTEE229 pKa = 4.36FGVSCKK235 pKa = 9.95PSSCMIHH242 pKa = 7.37GIEE245 pKa = 4.52CARR248 pKa = 11.84SQTSVSEE255 pKa = 4.25LYY257 pKa = 10.5VRR259 pKa = 11.84AFAVPSGADD268 pKa = 3.19PRR270 pKa = 11.84LYY272 pKa = 11.32DD273 pKa = 3.27MGNFYY278 pKa = 10.54IATQGMQGASVNVGEE293 pKa = 4.84LWVSYY298 pKa = 10.68DD299 pKa = 2.9ITFFKK304 pKa = 10.74AIEE307 pKa = 4.01QVPGFIMPLANYY319 pKa = 8.39TPVTASGTAPLGVTRR334 pKa = 11.84GISQPRR340 pKa = 11.84GVDD343 pKa = 3.41QIGLTFTEE351 pKa = 3.81NRR353 pKa = 11.84IIFPYY358 pKa = 9.98NIPIGSTYY366 pKa = 9.6QWTYY370 pKa = 10.03TISTASSALTLPVVTYY386 pKa = 11.08VNGFTDD392 pKa = 4.24LATYY396 pKa = 8.87KK397 pKa = 10.54APSNGEE403 pKa = 3.96VATKK407 pKa = 10.75GLISGMMRR415 pKa = 11.84LTSPGTPSTPPYY427 pKa = 10.9VNLADD432 pKa = 3.84FTVGAISFIALDD444 pKa = 3.64VAQISGFPAEE454 pKa = 4.25PP455 pKa = 3.51

Molecular weight: 48.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

904

449

455

452.0

50.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.965 ± 0.57

1.106 ± 0.308

6.416 ± 2.0

4.978 ± 1.614

3.982 ± 0.437

7.412 ± 2.316

2.102 ± 0.843

5.973 ± 0.124

6.969 ± 2.078

6.858 ± 1.244

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.323 ± 0.217

4.646 ± 0.324

5.531 ± 0.581

2.765 ± 0.821

4.314 ± 0.247

8.407 ± 1.174

5.973 ± 1.64

6.195 ± 0.73

1.659 ± 0.538

4.425 ± 0.586
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski