Lake Sarah-associated circular virus-4

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GA19|A0A126GA19_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-4 OX=1685768 PE=4 SV=1
MM1 pKa = 6.8TQRR4 pKa = 11.84ATCWSITINNPTDD17 pKa = 3.39EE18 pKa = 4.42EE19 pKa = 4.8TKK21 pKa = 10.57VALPVGWKK29 pKa = 8.48LTGQIEE35 pKa = 4.19QGEE38 pKa = 4.86EE39 pKa = 4.39GTVHH43 pKa = 5.06YY44 pKa = 10.62QGMLTTTQQRR54 pKa = 11.84FGSIKK59 pKa = 9.6VYY61 pKa = 10.16FPRR64 pKa = 11.84AHH66 pKa = 6.73IEE68 pKa = 3.66VARR71 pKa = 11.84NKK73 pKa = 10.04KK74 pKa = 9.75ALSEE78 pKa = 4.16YY79 pKa = 8.28VHH81 pKa = 7.22KK82 pKa = 10.95SDD84 pKa = 3.81TRR86 pKa = 11.84VAKK89 pKa = 10.7VDD91 pKa = 4.09DD92 pKa = 5.09NISNNIFQWQAEE104 pKa = 4.45VAKK107 pKa = 10.16LWNDD111 pKa = 2.98TDD113 pKa = 4.21FTHH116 pKa = 6.49MKK118 pKa = 10.09YY119 pKa = 10.12IYY121 pKa = 10.15DD122 pKa = 4.07KK123 pKa = 11.08EE124 pKa = 4.16PSEE127 pKa = 4.84DD128 pKa = 3.45VALKK132 pKa = 10.82YY133 pKa = 10.61VDD135 pKa = 4.0SLCGKK140 pKa = 9.91LIRR143 pKa = 11.84EE144 pKa = 4.29GAKK147 pKa = 10.0GLEE150 pKa = 4.21FVAVNPMWRR159 pKa = 11.84SSWKK163 pKa = 9.8RR164 pKa = 11.84FYY166 pKa = 10.99FSIITRR172 pKa = 11.84DD173 pKa = 3.51GRR175 pKa = 11.84DD176 pKa = 3.11EE177 pKa = 4.29AVCEE181 pKa = 4.18TQVPQGEE188 pKa = 4.38EE189 pKa = 4.08TNEE192 pKa = 4.01KK193 pKa = 10.74GSS195 pKa = 3.46

Molecular weight: 22.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GA19|A0A126GA19_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-4 OX=1685768 PE=4 SV=1
MM1 pKa = 7.67GGMKK5 pKa = 9.87QYY7 pKa = 11.48AKK9 pKa = 10.29RR10 pKa = 11.84KK11 pKa = 7.23YY12 pKa = 10.11RR13 pKa = 11.84KK14 pKa = 9.28GKK16 pKa = 10.01KK17 pKa = 6.77PTKK20 pKa = 10.0KK21 pKa = 10.11GVKK24 pKa = 9.19INYY27 pKa = 9.19ADD29 pKa = 3.4QHH31 pKa = 5.18NLKK34 pKa = 10.01VQGTSCFIWTSGSYY48 pKa = 10.5PSVQANNALCVTVNPEE64 pKa = 3.98LRR66 pKa = 11.84PPNLSVFAFGGALQFSLGNAINLAALATYY95 pKa = 9.13WDD97 pKa = 4.44RR98 pKa = 11.84IKK100 pKa = 11.22CNEE103 pKa = 3.7IKK105 pKa = 10.82VRR107 pKa = 11.84VIPQINFASVGSGLIPTMRR126 pKa = 11.84TVFDD130 pKa = 4.12FDD132 pKa = 4.73DD133 pKa = 4.22AVTPTSSQMWARR145 pKa = 11.84RR146 pKa = 11.84GKK148 pKa = 6.81THH150 pKa = 7.66RR151 pKa = 11.84LDD153 pKa = 3.51KK154 pKa = 10.96PFTFSFKK161 pKa = 10.52PRR163 pKa = 11.84VLYY166 pKa = 10.32TGVTALSLSQKK177 pKa = 10.07SPYY180 pKa = 9.02MNNASALTIPLYY192 pKa = 10.39GVKK195 pKa = 10.28FGVRR199 pKa = 11.84DD200 pKa = 3.62WPLQAPTDD208 pKa = 4.06PLPNILRR215 pKa = 11.84FEE217 pKa = 4.09ITYY220 pKa = 8.13MCTMKK225 pKa = 10.28EE226 pKa = 4.05QQWINTPVNIADD238 pKa = 3.99YY239 pKa = 10.88GVLLPGQVAPVANEE253 pKa = 3.68VMDD256 pKa = 3.73VSGNIYY262 pKa = 10.32DD263 pKa = 4.87LSGNMIKK270 pKa = 10.67APDD273 pKa = 3.55EE274 pKa = 4.71FGWEE278 pKa = 4.32HH279 pKa = 7.16PWTGPTGLPYY289 pKa = 10.28TGPTGPGPCYY299 pKa = 10.45CC300 pKa = 5.15

Molecular weight: 33.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

495

195

300

247.5

27.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.667 ± 0.319

1.818 ± 0.174

4.646 ± 0.618

4.848 ± 2.406

4.04 ± 0.28

7.475 ± 0.821

1.414 ± 0.396

5.253 ± 0.242

6.869 ± 0.512

6.263 ± 1.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.626 ± 0.358

5.455 ± 0.522

6.465 ± 2.107

4.444 ± 0.425

4.444 ± 0.425

5.657 ± 0.01

7.677 ± 0.329

7.273 ± 0.261

2.626 ± 0.28

4.04 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski