Pseudomonas phage ZC01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Abidjanvirus; unclassified Abidjanvirus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L2C8W3|A0A1L2C8W3_9CAUD Uncharacterized protein OS=Pseudomonas phage ZC01 OX=1622114 GN=ZC01_039 PE=4 SV=1
MM1 pKa = 7.42NYY3 pKa = 10.15EE4 pKa = 3.8MTKK7 pKa = 9.99ILVAALVADD16 pKa = 4.57TSATVEE22 pKa = 3.94WAQDD26 pKa = 3.59VLGGDD31 pKa = 3.73AYY33 pKa = 10.67GVAVTLGDD41 pKa = 3.77KK42 pKa = 10.96LFLVYY47 pKa = 10.78QMDD50 pKa = 4.25NNPQEE55 pKa = 4.25DD56 pKa = 3.72WLVDD60 pKa = 3.53VMEE63 pKa = 4.98TEE65 pKa = 5.13GEE67 pKa = 3.98MAYY70 pKa = 10.78CPDD73 pKa = 3.38VQGFASQAAALRR85 pKa = 11.84YY86 pKa = 8.77LASKK90 pKa = 9.76FF91 pKa = 3.53

Molecular weight: 9.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L2C8X1|A0A1L2C8X1_9CAUD Uncharacterized protein OS=Pseudomonas phage ZC01 OX=1622114 GN=ZC01_036 PE=4 SV=1
MM1 pKa = 7.25NLDD4 pKa = 4.65TILLWSGWATLVLGVLFSLASGLGLVLVLLDD35 pKa = 3.91YY36 pKa = 10.88LGKK39 pKa = 10.3RR40 pKa = 11.84LWHH43 pKa = 7.09RR44 pKa = 11.84ILKK47 pKa = 10.23FYY49 pKa = 10.63DD50 pKa = 3.15LHH52 pKa = 5.56EE53 pKa = 4.29VARR56 pKa = 11.84VVRR59 pKa = 11.84EE60 pKa = 3.96AEE62 pKa = 4.23LEE64 pKa = 4.18GKK66 pKa = 8.04LTRR69 pKa = 11.84RR70 pKa = 11.84APPEE74 pKa = 3.8PPRR77 pKa = 11.84KK78 pKa = 9.46RR79 pKa = 11.84RR80 pKa = 11.84GLWKK84 pKa = 10.37KK85 pKa = 10.44

Molecular weight: 9.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

17685

55

826

229.7

25.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.567 ± 0.448

1.086 ± 0.104

5.722 ± 0.185

7.611 ± 0.346

3.687 ± 0.164

7.809 ± 0.262

1.968 ± 0.163

3.958 ± 0.136

4.874 ± 0.231

9.285 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.126 ± 0.117

3.421 ± 0.152

4.806 ± 0.256

3.998 ± 0.172

6.87 ± 0.256

5.541 ± 0.268

5.428 ± 0.278

6.966 ± 0.215

2.053 ± 0.153

2.884 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski