Streptomyces phage Austintatious

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Austintatiousvirus; Streptomyces virus Austintatious

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411AXI7|A0A411AXI7_9CAUD SsDNA binding protein OS=Streptomyces phage Austintatious OX=2500795 GN=43 PE=3 SV=1
MM1 pKa = 6.55TTYY4 pKa = 8.15PTANLGHH11 pKa = 6.99LQTLADD17 pKa = 4.45DD18 pKa = 4.6PAAHH22 pKa = 6.78RR23 pKa = 11.84AVLYY27 pKa = 10.94LDD29 pKa = 5.21DD30 pKa = 6.47DD31 pKa = 4.16NDD33 pKa = 4.1LAVGADD39 pKa = 2.88IHH41 pKa = 6.24AAPGRR46 pKa = 11.84VLLTAEE52 pKa = 3.98QLGDD56 pKa = 3.48NGGPIDD62 pKa = 5.71DD63 pKa = 4.51EE64 pKa = 4.46TTEE67 pKa = 4.23ALLDD71 pKa = 4.61EE72 pKa = 5.13INDD75 pKa = 4.42DD76 pKa = 3.51IEE78 pKa = 6.43RR79 pKa = 11.84ILDD82 pKa = 3.63EE83 pKa = 5.99DD84 pKa = 4.18DD85 pKa = 3.37

Molecular weight: 9.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411AXI2|A0A411AXI2_9CAUD Uncharacterized protein OS=Streptomyces phage Austintatious OX=2500795 GN=50 PE=4 SV=1
MM1 pKa = 6.86NTPEE5 pKa = 3.94RR6 pKa = 11.84FAAKK10 pKa = 9.79VDD12 pKa = 3.69PAGPLSLYY20 pKa = 10.21RR21 pKa = 11.84DD22 pKa = 3.77APGPCHH28 pKa = 7.32LWTGGARR35 pKa = 11.84SKK37 pKa = 10.65RR38 pKa = 11.84PHH40 pKa = 6.89DD41 pKa = 4.26AGEE44 pKa = 4.03HH45 pKa = 5.75GEE47 pKa = 4.09FYY49 pKa = 11.07GAFKK53 pKa = 10.96ADD55 pKa = 3.06GRR57 pKa = 11.84TVRR60 pKa = 11.84AHH62 pKa = 5.33QYY64 pKa = 10.54AYY66 pKa = 9.5EE67 pKa = 4.14QARR70 pKa = 11.84GPIPTGAEE78 pKa = 3.36VDD80 pKa = 3.5HH81 pKa = 6.78RR82 pKa = 11.84CRR84 pKa = 11.84RR85 pKa = 11.84RR86 pKa = 11.84NCVNPAHH93 pKa = 7.05LEE95 pKa = 4.35VTDD98 pKa = 3.78HH99 pKa = 6.22RR100 pKa = 11.84TNTLRR105 pKa = 11.84SSGPTAINARR115 pKa = 11.84KK116 pKa = 8.8VRR118 pKa = 11.84CLRR121 pKa = 11.84GHH123 pKa = 7.02PFDD126 pKa = 4.98AANTYY131 pKa = 9.66IRR133 pKa = 11.84PNGARR138 pKa = 11.84ACRR141 pKa = 11.84ACRR144 pKa = 11.84RR145 pKa = 11.84PATEE149 pKa = 3.6RR150 pKa = 11.84TAAA153 pKa = 3.7

Molecular weight: 16.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11414

48

805

215.4

23.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.385 ± 0.601

0.561 ± 0.113

6.886 ± 0.356

5.87 ± 0.277

1.647 ± 0.138

8.402 ± 0.547

2.05 ± 0.211

3.461 ± 0.311

1.989 ± 0.212

8.341 ± 0.333

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.814 ± 0.184

1.857 ± 0.132

6.387 ± 0.428

4.056 ± 0.291

8.919 ± 0.536

4.67 ± 0.308

7.631 ± 0.315

6.229 ± 0.294

1.77 ± 0.194

2.076 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski