Agrobacterium radiobacter (strain K84 / ATCC BAA-868)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium; Agrobacterium tumefaciens complex; Agrobacterium tumefaciens

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6674 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B9J9H3|B9J9H3_AGRRK Cell division coordinator CpoB OS=Agrobacterium radiobacter (strain K84 / ATCC BAA-868) OX=311403 GN=ygbF PE=3 SV=1
MM1 pKa = 7.61SIFGSMKK8 pKa = 9.46TAISGMNAQANRR20 pKa = 11.84LATVSDD26 pKa = 4.49NIANSSTTGYY36 pKa = 9.56KK37 pKa = 9.37QASTSFSSLVLPSSSGNYY55 pKa = 9.32NSGGVEE61 pKa = 3.84TTVRR65 pKa = 11.84YY66 pKa = 9.69AISQQGTVSATTSSTDD82 pKa = 4.25LAIQGNGFFVVQSAAGQTFLTRR104 pKa = 11.84SGDD107 pKa = 3.69FSADD111 pKa = 3.01ASGNLVNAAGFTLMGYY127 pKa = 9.77SSASGAPSVVVNGFSGMVPINVTPSGTSVAATTVGSMTGNLNSNATIATSSSTGYY182 pKa = 10.52LPSEE186 pKa = 3.74NTYY189 pKa = 10.67PVTTDD194 pKa = 3.14TSKK197 pKa = 11.38SSITGYY203 pKa = 10.54DD204 pKa = 3.3SQGNAVTFDD213 pKa = 2.93IYY215 pKa = 9.5YY216 pKa = 9.75TKK218 pKa = 10.29TADD221 pKa = 3.66NTWDD225 pKa = 3.44VQVYY229 pKa = 9.27NQADD233 pKa = 4.11LVASPTSGGPLYY245 pKa = 10.94SSAAVGSTEE254 pKa = 3.88MTFDD258 pKa = 4.49DD259 pKa = 5.58DD260 pKa = 4.04GALVSGGTFDD270 pKa = 5.77LSFTSGSTTQSIAMDD285 pKa = 3.5MTGFTQVATDD295 pKa = 4.39FSATGKK301 pKa = 9.59MDD303 pKa = 3.52GQAPNPVTSVTIAKK317 pKa = 9.85DD318 pKa = 3.51GTVSAVYY325 pKa = 10.02KK326 pKa = 10.71DD327 pKa = 3.87SSTKK331 pKa = 9.33EE332 pKa = 3.86LYY334 pKa = 10.14RR335 pKa = 11.84IPLATVASPDD345 pKa = 3.62SLTLEE350 pKa = 4.27SGNVYY355 pKa = 10.22SANGDD360 pKa = 3.45SGVTVTGWPQSGGLGYY376 pKa = 9.71IQSGALEE383 pKa = 4.23EE384 pKa = 4.93SNVDD388 pKa = 4.04LATEE392 pKa = 4.61LTNMITAQKK401 pKa = 10.63SYY403 pKa = 9.31TANSKK408 pKa = 9.73VFQAGSDD415 pKa = 3.75LLDD418 pKa = 3.26VLVNLQRR425 pKa = 4.57

Molecular weight: 43.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B9JP40|B9JP40_AGRRK Chaperone protein ClpB OS=Agrobacterium radiobacter (strain K84 / ATCC BAA-868) OX=311403 GN=clpB PE=3 SV=1
MM1 pKa = 7.61SEE3 pKa = 3.66TMLAVLSNIRR13 pKa = 11.84TIDD16 pKa = 3.52DD17 pKa = 3.23MVAAFRR23 pKa = 11.84EE24 pKa = 4.47EE25 pKa = 4.07EE26 pKa = 3.84HH27 pKa = 6.68CRR29 pKa = 11.84RR30 pKa = 11.84LLEE33 pKa = 4.26SMVWRR38 pKa = 11.84DD39 pKa = 3.27GRR41 pKa = 11.84VCPACGYY48 pKa = 9.65KK49 pKa = 10.15RR50 pKa = 11.84SIAIAGRR57 pKa = 11.84DD58 pKa = 3.27MGKK61 pKa = 9.66RR62 pKa = 11.84RR63 pKa = 11.84ARR65 pKa = 11.84PGLYY69 pKa = 9.1QCSSGDD75 pKa = 3.38CRR77 pKa = 11.84FQFTVTTHH85 pKa = 6.24TPLHH89 pKa = 5.59ATKK92 pKa = 10.68LPLRR96 pKa = 11.84TWLKK100 pKa = 11.29AMWLLLQSDD109 pKa = 4.25KK110 pKa = 11.35GLSSVRR116 pKa = 11.84LAEE119 pKa = 4.18TLGVSQPTAWRR130 pKa = 11.84IGHH133 pKa = 7.03ALRR136 pKa = 11.84LMVARR141 pKa = 11.84EE142 pKa = 3.87HH143 pKa = 6.3MLDD146 pKa = 3.26GTVEE150 pKa = 3.75VDD152 pKa = 2.93HH153 pKa = 7.06FYY155 pKa = 11.35LGGRR159 pKa = 11.84PGKK162 pKa = 10.08HH163 pKa = 6.68PDD165 pKa = 3.76DD166 pKa = 4.84PPPGRR171 pKa = 11.84GRR173 pKa = 11.84KK174 pKa = 8.68GQAKK178 pKa = 5.64TQKK181 pKa = 8.29TPVMAIVQRR190 pKa = 11.84PADD193 pKa = 3.86VTPGSPAGDD202 pKa = 3.0ARR204 pKa = 11.84AAVVTGLSLRR214 pKa = 11.84AAVGVIAPQVEE225 pKa = 4.36LHH227 pKa = 6.02AHH229 pKa = 6.05LMSDD233 pKa = 3.4EE234 pKa = 4.33ANAFVAIGEE243 pKa = 4.37SFAVHH248 pKa = 5.02EE249 pKa = 4.75TVNHH253 pKa = 5.93SSRR256 pKa = 11.84EE257 pKa = 4.03YY258 pKa = 10.52VRR260 pKa = 11.84NTVHH264 pKa = 6.19VNSAEE269 pKa = 3.98GFNARR274 pKa = 11.84VRR276 pKa = 11.84RR277 pKa = 11.84TIAGVFHH284 pKa = 7.07HH285 pKa = 7.17ISPEE289 pKa = 3.79LADD292 pKa = 4.86LYY294 pKa = 10.42FHH296 pKa = 7.44EE297 pKa = 5.62IGFRR301 pKa = 11.84WSQRR305 pKa = 11.84VVIGQAVRR313 pKa = 11.84RR314 pKa = 11.84NRR316 pKa = 11.84SGKK319 pKa = 10.05EE320 pKa = 3.34SGKK323 pKa = 9.89ILWSRR328 pKa = 11.84VPPALQLLQVFRR340 pKa = 11.84AATGRR345 pKa = 11.84QMRR348 pKa = 11.84RR349 pKa = 11.84SHH351 pKa = 6.14QGGITIKK358 pKa = 9.83STVAVFGG365 pKa = 3.86

Molecular weight: 40.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6674

0

6674

2107362

30

4272

315.8

34.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.653 ± 0.043

0.785 ± 0.009

5.674 ± 0.022

5.486 ± 0.032

3.93 ± 0.022

8.273 ± 0.032

2.058 ± 0.014

5.929 ± 0.022

3.718 ± 0.025

10.051 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.521 ± 0.015

2.933 ± 0.023

4.861 ± 0.02

3.2 ± 0.021

6.513 ± 0.036

5.993 ± 0.024

5.477 ± 0.026

7.29 ± 0.021

1.291 ± 0.012

2.363 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski