Prevotella pleuritidis F0068

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; Prevotella pleuritidis

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2257 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U2L430|U2L430_9BACT Uncharacterized protein OS=Prevotella pleuritidis F0068 OX=1081904 GN=HMPREF1218_1173 PE=4 SV=1
MM1 pKa = 6.95WQFAEE6 pKa = 4.11KK7 pKa = 9.94TYY9 pKa = 11.08INNDD13 pKa = 3.13LKK15 pKa = 11.35NDD17 pKa = 3.82DD18 pKa = 4.74NEE20 pKa = 4.38TEE22 pKa = 3.93IYY24 pKa = 11.17LMVLCLASDD33 pKa = 3.6GHH35 pKa = 7.49VDD37 pKa = 3.95GVV39 pKa = 3.54

Molecular weight: 4.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U2LGS1|U2LGS1_9BACT AAA domain protein OS=Prevotella pleuritidis F0068 OX=1081904 GN=HMPREF1218_0083 PE=4 SV=1
MM1 pKa = 7.12NRR3 pKa = 11.84ASEE6 pKa = 4.08RR7 pKa = 11.84QIPGCSILLHH17 pKa = 6.41PGISDD22 pKa = 3.17IQDD25 pKa = 3.48LVRR28 pKa = 11.84TSKK31 pKa = 10.32VCRR34 pKa = 11.84CDD36 pKa = 2.9PRR38 pKa = 11.84TRR40 pKa = 11.84RR41 pKa = 11.84NRR43 pKa = 11.84FHH45 pKa = 6.74LHH47 pKa = 6.51KK48 pKa = 10.08KK49 pKa = 9.88RR50 pKa = 11.84CACTPRR56 pKa = 11.84VGYY59 pKa = 10.0FKK61 pKa = 10.92GALVGYY67 pKa = 9.89HH68 pKa = 5.55RR69 pKa = 11.84TPLVGCVYY77 pKa = 10.53RR78 pKa = 11.84SLVGCNIIPFTNNYY92 pKa = 9.26NSSNSRR98 pKa = 11.84YY99 pKa = 8.97FNLSKK104 pKa = 9.85TVADD108 pKa = 4.21TKK110 pKa = 10.84RR111 pKa = 11.84SATVLSS117 pKa = 3.98

Molecular weight: 13.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2257

0

2257

784947

31

2505

347.8

39.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.277 ± 0.059

1.253 ± 0.018

5.75 ± 0.038

5.926 ± 0.057

4.505 ± 0.035

6.735 ± 0.05

2.094 ± 0.024

6.92 ± 0.045

6.301 ± 0.047

9.076 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.692 ± 0.025

4.961 ± 0.04

3.623 ± 0.025

3.656 ± 0.027

5.535 ± 0.045

5.96 ± 0.04

5.855 ± 0.037

6.464 ± 0.044

1.172 ± 0.02

4.245 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski