Legionella shakespearei DSM 23087

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Legionellaceae; Legionella; Legionella shakespearei

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2916 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0W0YLC6|A0A0W0YLC6_9GAMM Endonuclease III OS=Legionella shakespearei DSM 23087 OX=1122169 GN=nth PE=3 SV=1
MM1 pKa = 7.44AAVDD5 pKa = 4.52ILPTTNDD12 pKa = 2.52IYY14 pKa = 11.14FSVSAADD21 pKa = 4.17KK22 pKa = 10.32VSEE25 pKa = 4.85LIKK28 pKa = 11.12EE29 pKa = 4.09EE30 pKa = 4.97DD31 pKa = 3.63NLNLNLRR38 pKa = 11.84VSITGGGCSGFQYY51 pKa = 10.64GFSFDD56 pKa = 4.01EE57 pKa = 5.43EE58 pKa = 4.32INEE61 pKa = 4.67DD62 pKa = 3.56DD63 pKa = 4.38TIIEE67 pKa = 4.38QKK69 pKa = 10.81CSDD72 pKa = 3.38GSSTVKK78 pKa = 10.79LLVDD82 pKa = 3.66SMSYY86 pKa = 10.53QYY88 pKa = 11.57LHH90 pKa = 7.17DD91 pKa = 4.78AEE93 pKa = 4.61IDD95 pKa = 3.96YY96 pKa = 10.88IQGIQGEE103 pKa = 4.48QFVIRR108 pKa = 11.84NPNAKK113 pKa = 6.55TTCGCGSSFSMDD125 pKa = 4.89DD126 pKa = 3.7EE127 pKa = 6.97DD128 pKa = 6.12DD129 pKa = 3.7LL130 pKa = 6.64

Molecular weight: 14.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0W0YZX1|A0A0W0YZX1_9GAMM Prophage CP4-57 regulatory protein (AlpA) OS=Legionella shakespearei DSM 23087 OX=1122169 GN=Lsha_1108 PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 10.14INSRR6 pKa = 11.84IKK8 pKa = 10.61AIAVLLASTILTGCVVAVVAGAAAGLVYY36 pKa = 10.48DD37 pKa = 4.29RR38 pKa = 11.84RR39 pKa = 11.84SVTTLEE45 pKa = 3.57ADD47 pKa = 2.89ARR49 pKa = 11.84LFHH52 pKa = 6.69VIHH55 pKa = 6.67KK56 pKa = 9.88GIVSNPQFRR65 pKa = 11.84DD66 pKa = 2.97SRR68 pKa = 11.84ILVTSFNRR76 pKa = 11.84VVLLVGQTPTASLRR90 pKa = 11.84VVAEE94 pKa = 5.28RR95 pKa = 11.84IAQSAPGVKK104 pKa = 8.62RR105 pKa = 11.84VYY107 pKa = 10.97DD108 pKa = 4.72EE109 pKa = 4.79ISVDD113 pKa = 3.57NPLPLTQRR121 pKa = 11.84SKK123 pKa = 11.42DD124 pKa = 3.22SWITGQVRR132 pKa = 11.84SNMLTRR138 pKa = 11.84KK139 pKa = 9.38GLEE142 pKa = 3.92SGSIRR147 pKa = 11.84IVTEE151 pKa = 3.49NGVVYY156 pKa = 10.69LMGIVTQEE164 pKa = 3.86QASLSVDD171 pKa = 3.16VARR174 pKa = 11.84RR175 pKa = 11.84INGVRR180 pKa = 11.84KK181 pKa = 9.14VVKK184 pKa = 9.21IFQYY188 pKa = 10.51IRR190 pKa = 3.21

Molecular weight: 20.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2916

0

2916

998065

50

4593

342.3

38.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.0 ± 0.048

1.152 ± 0.015

5.144 ± 0.036

6.021 ± 0.05

4.374 ± 0.037

6.112 ± 0.042

2.553 ± 0.027

6.899 ± 0.042

5.911 ± 0.04

10.939 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.475 ± 0.022

4.726 ± 0.038

4.181 ± 0.029

4.599 ± 0.04

4.287 ± 0.034

6.696 ± 0.036

5.28 ± 0.043

6.137 ± 0.04

1.114 ± 0.017

3.395 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski