Corynebacterium sp. HMSC08D02

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Corynebacteriaceae; Corynebacterium; unclassified Corynebacterium

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2124 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1F1UXR2|A0A1F1UXR2_9CORY tRNA glutamyl-Q synthetase OS=Corynebacterium sp. HMSC08D02 OX=1581138 GN=HMPREF3170_01380 PE=3 SV=1
MM1 pKa = 7.35MSLNPDD7 pKa = 3.84FYY9 pKa = 11.34YY10 pKa = 10.73SEE12 pKa = 3.87DD13 pKa = 3.94HH14 pKa = 5.92EE15 pKa = 5.03WINARR20 pKa = 11.84PEE22 pKa = 3.93DD23 pKa = 3.65AAGTKK28 pKa = 10.06VRR30 pKa = 11.84VGITNVAADD39 pKa = 4.0RR40 pKa = 11.84LGEE43 pKa = 4.25VVFAEE48 pKa = 4.78LPQVGDD54 pKa = 3.28QVTAGEE60 pKa = 4.44TCGEE64 pKa = 4.21VEE66 pKa = 4.36STKK69 pKa = 10.75SVSDD73 pKa = 3.74LYY75 pKa = 11.51APVTGTVTAVNEE87 pKa = 5.03DD88 pKa = 2.77IDD90 pKa = 4.08GAYY93 pKa = 9.85EE94 pKa = 5.06AINEE98 pKa = 4.25DD99 pKa = 3.77PFGAGWLFEE108 pKa = 4.38VEE110 pKa = 4.24VEE112 pKa = 4.25EE113 pKa = 4.94VGPLMTADD121 pKa = 4.24EE122 pKa = 4.3YY123 pKa = 11.66AAANGVEE130 pKa = 4.09NN131 pKa = 4.31

Molecular weight: 14.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1F1UVE9|A0A1F1UVE9_9CORY Thioredoxin reductase OS=Corynebacterium sp. HMSC08D02 OX=1581138 GN=HMPREF3170_05425 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SRR15 pKa = 11.84KK16 pKa = 8.14HH17 pKa = 4.83GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVSARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.63KK38 pKa = 10.53GRR40 pKa = 11.84ASLTAA45 pKa = 4.1

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2124

0

2124

703705

40

2502

331.3

35.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.627 ± 0.082

0.701 ± 0.015

6.039 ± 0.049

6.527 ± 0.054

3.342 ± 0.033

8.16 ± 0.053

2.14 ± 0.027

4.899 ± 0.038

3.459 ± 0.052

9.349 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.255 ± 0.026

2.91 ± 0.036

5.102 ± 0.046

3.58 ± 0.032

6.054 ± 0.06

5.558 ± 0.039

6.221 ± 0.054

8.433 ± 0.054

1.363 ± 0.021

2.282 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski