Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Buchnera; Buchnera aphidicola; Buchnera aphidicola (Baizongia pistaciae)

Average proteome isoelectric point is 8.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 507 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P59450|MURB_BUCBP UDP-N-acetylenolpyruvoylglucosamine reductase OS=Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) OX=224915 GN=murB PE=3 SV=1
MM1 pKa = 7.31QNIEE5 pKa = 4.38KK6 pKa = 10.18KK7 pKa = 9.1IKK9 pKa = 10.3KK10 pKa = 9.84IIAQQLGLIEE20 pKa = 3.97QKK22 pKa = 8.74ITNEE26 pKa = 3.92SLLVEE31 pKa = 4.68DD32 pKa = 5.64LNADD36 pKa = 3.47SLDD39 pKa = 3.41IVEE42 pKa = 5.79LIMAFEE48 pKa = 4.33EE49 pKa = 4.34EE50 pKa = 4.37FNIEE54 pKa = 3.95ITDD57 pKa = 3.77EE58 pKa = 3.98EE59 pKa = 4.85AEE61 pKa = 4.26NLTSVQSIFDD71 pKa = 3.95IIKK74 pKa = 10.19KK75 pKa = 10.14YY76 pKa = 10.75VFF78 pKa = 3.69

Molecular weight: 9.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q89B45|ATP6_BUCBP ATP synthase subunit a OS=Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) OX=224915 GN=atpB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTLKK11 pKa = 10.58RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 6.97GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.89NGRR28 pKa = 11.84HH29 pKa = 4.78ILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.55FRR39 pKa = 11.84TRR41 pKa = 11.84LTVSSNN47 pKa = 2.94

Molecular weight: 5.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

507

0

507

166799

38

1404

329.0

37.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.151 ± 0.079

1.473 ± 0.044

4.029 ± 0.074

4.516 ± 0.088

5.032 ± 0.099

5.253 ± 0.089

2.26 ± 0.048

11.721 ± 0.114

9.573 ± 0.123

10.161 ± 0.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.207 ± 0.043

7.661 ± 0.11

2.924 ± 0.052

3.151 ± 0.052

3.671 ± 0.084

7.392 ± 0.083

4.915 ± 0.074

5.232 ± 0.093

0.903 ± 0.039

3.773 ± 0.065
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski