Leeuwenhoekiella blandensis (strain CECT 7118 / CCUG 51940 / MED217) (Flavobacterium sp. (strain MED217))

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Leeuwenhoekiella; Leeuwenhoekiella blandensis

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3687 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3XJ84|A3XJ84_LEEBM Phosphoheptose isomerase OS=Leeuwenhoekiella blandensis (strain CECT 7118 / CCUG 51940 / MED217) OX=398720 GN=MED217_05157 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.16KK3 pKa = 10.41LLIAFVCIAGFSFSSQAQDD22 pKa = 2.94IAPNALGLRR31 pKa = 11.84LGDD34 pKa = 3.27SDD36 pKa = 4.76GFGAEE41 pKa = 3.55ISYY44 pKa = 10.25QRR46 pKa = 11.84ALGSNNRR53 pKa = 11.84LEE55 pKa = 4.57LDD57 pKa = 3.4LGIRR61 pKa = 11.84DD62 pKa = 3.65GNNYY66 pKa = 10.67DD67 pKa = 3.65GFKK70 pKa = 10.74LAGLYY75 pKa = 9.28QWVWNIDD82 pKa = 3.55GGFNWYY88 pKa = 10.41AGVGGGLASYY98 pKa = 10.64DD99 pKa = 3.95YY100 pKa = 11.37DD101 pKa = 5.21RR102 pKa = 11.84NDD104 pKa = 3.36YY105 pKa = 11.56DD106 pKa = 5.67DD107 pKa = 4.11DD108 pKa = 4.28TFVFAAGDD116 pKa = 3.24IGIEE120 pKa = 3.99YY121 pKa = 10.6DD122 pKa = 3.5FDD124 pKa = 4.34IPLVLSLDD132 pKa = 3.66FRR134 pKa = 11.84PEE136 pKa = 3.39IGFGDD141 pKa = 3.74GNNYY145 pKa = 9.57YY146 pKa = 11.03DD147 pKa = 5.63NDD149 pKa = 4.34DD150 pKa = 4.27LDD152 pKa = 4.51FDD154 pKa = 3.78IALGIRR160 pKa = 11.84YY161 pKa = 7.87QFF163 pKa = 3.65

Molecular weight: 18.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3XMM6|A3XMM6_LEEBM 30S ribosomal protein S1 OS=Leeuwenhoekiella blandensis (strain CECT 7118 / CCUG 51940 / MED217) OX=398720 GN=MED217_07311 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.13RR10 pKa = 11.84KK11 pKa = 9.48RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.49HH16 pKa = 3.94GFRR19 pKa = 11.84EE20 pKa = 4.27RR21 pKa = 11.84MASANGRR28 pKa = 11.84KK29 pKa = 9.04VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.29GRR39 pKa = 11.84KK40 pKa = 8.32RR41 pKa = 11.84ISVSSEE47 pKa = 3.46LRR49 pKa = 11.84HH50 pKa = 6.16KK51 pKa = 10.53KK52 pKa = 10.04

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3687

0

3687

1268469

21

3863

344.0

38.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.431 ± 0.041

0.66 ± 0.011

5.753 ± 0.036

6.968 ± 0.039

5.078 ± 0.031

6.52 ± 0.045

1.767 ± 0.022

7.003 ± 0.033

6.877 ± 0.057

9.657 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.114 ± 0.02

5.456 ± 0.037

3.58 ± 0.022

3.835 ± 0.025

3.804 ± 0.031

6.185 ± 0.031

5.86 ± 0.05

6.226 ± 0.037

1.093 ± 0.014

4.134 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski