Escherichia phage vB_EcoS_ESCO41

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Nouzillyvirus; Escherichia virus ESCO41

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U9WR45|A0A1U9WR45_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_ESCO41 OX=2496547 GN=ESCO41_00020 PE=4 SV=1
MM1 pKa = 7.42VKK3 pKa = 10.19LFCVKK8 pKa = 9.03NTSKK12 pKa = 9.61TLPFTVNNPYY22 pKa = 10.09SAEE25 pKa = 3.98YY26 pKa = 10.2QGDD29 pKa = 4.1GNYY32 pKa = 10.33KK33 pKa = 10.05IYY35 pKa = 10.64GDD37 pKa = 3.72DD38 pKa = 3.66MTWIFAPIDD47 pKa = 3.6GALVEE52 pKa = 5.97FIIADD57 pKa = 3.49

Molecular weight: 6.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U9WR60|A0A1U9WR60_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_ESCO41 OX=2496547 GN=ESCO41_00026 PE=4 SV=1
MM1 pKa = 7.4SIVYY5 pKa = 9.27GVIYY9 pKa = 10.33KK10 pKa = 10.57GIADD14 pKa = 4.07GKK16 pKa = 8.54TRR18 pKa = 11.84FITPSGRR25 pKa = 11.84MYY27 pKa = 10.12VWSYY31 pKa = 9.55GAWRR35 pKa = 11.84FYY37 pKa = 10.84YY38 pKa = 10.33QFVDD42 pKa = 3.11STYY45 pKa = 10.59RR46 pKa = 11.84LIRR49 pKa = 11.84SAVGKK54 pKa = 10.22KK55 pKa = 9.77GSKK58 pKa = 10.19VFLASSRR65 pKa = 11.84KK66 pKa = 8.54VFMEE70 pKa = 3.82KK71 pKa = 9.75RR72 pKa = 11.84VALDD76 pKa = 4.27PIDD79 pKa = 3.86YY80 pKa = 10.46LAVEE84 pKa = 4.23FMGRR88 pKa = 11.84RR89 pKa = 11.84FSKK92 pKa = 10.48EE93 pKa = 3.34RR94 pKa = 11.84LFRR97 pKa = 11.84NGEE100 pKa = 3.83IARR103 pKa = 11.84VQQNAAVALGKK114 pKa = 10.09AARR117 pKa = 11.84QNLTGYY123 pKa = 8.77QPCFPPTPFYY133 pKa = 11.19SHH135 pKa = 6.58YY136 pKa = 10.42KK137 pKa = 10.02RR138 pKa = 11.84PPKK141 pKa = 10.48NRR143 pKa = 3.34

Molecular weight: 16.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

15440

52

1126

195.4

21.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.83 ± 0.376

1.554 ± 0.198

6.114 ± 0.213

6.386 ± 0.268

4.1 ± 0.227

7.921 ± 0.218

1.813 ± 0.178

6.729 ± 0.208

7.189 ± 0.395

6.47 ± 0.253

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.044 ± 0.213

5.104 ± 0.221

3.374 ± 0.216

3.446 ± 0.209

4.722 ± 0.237

6.755 ± 0.386

5.168 ± 0.244

7.073 ± 0.306

1.451 ± 0.116

3.756 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski