Aminipila sp. JN-18

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Eubacteriales Family XIII. Incertae Sedis; Aminipila; unclassified Aminipila

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2438 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A410PXX9|A0A410PXX9_9FIRM AraC family transcriptional regulator OS=Aminipila sp. JN-18 OX=2507160 GN=EQM06_11595 PE=4 SV=1
MM1 pKa = 7.82KK2 pKa = 10.06KK3 pKa = 9.97NQMPQIGLPADD14 pKa = 3.3ACEE17 pKa = 4.21RR18 pKa = 11.84SGFTDD23 pKa = 3.35KK24 pKa = 10.84DD25 pKa = 3.73TLEE28 pKa = 3.99LHH30 pKa = 7.01AGQNALVFMKK40 pKa = 10.74DD41 pKa = 2.57KK42 pKa = 8.84MTALEE47 pKa = 4.16VANAIQSLSALAADD61 pKa = 4.25LTVVLASACGLCDD74 pKa = 3.42NCGEE78 pKa = 4.47GCADD82 pKa = 4.44DD83 pKa = 5.77CPAGCVSACSLCHH96 pKa = 6.99DD97 pKa = 5.18LLDD100 pKa = 4.0EE101 pKa = 4.52SQTVRR106 pKa = 11.84IPGYY110 pKa = 9.85LLEE113 pKa = 4.57EE114 pKa = 4.43AGIPADD120 pKa = 4.23AKK122 pKa = 10.99LEE124 pKa = 4.35AYY126 pKa = 9.16TDD128 pKa = 3.78EE129 pKa = 5.13DD130 pKa = 3.42SGEE133 pKa = 4.15ITVVEE138 pKa = 4.56ADD140 pKa = 3.28IQQDD144 pKa = 3.26ITDD147 pKa = 4.06VPPGILAVLAQSGVCLAEE165 pKa = 4.23LDD167 pKa = 4.12EE168 pKa = 6.44LIMLDD173 pKa = 4.24SIIYY177 pKa = 10.25GNN179 pKa = 3.57

Molecular weight: 18.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A410PTJ9|A0A410PTJ9_9FIRM DUF5049 domain-containing protein OS=Aminipila sp. JN-18 OX=2507160 GN=EQM06_03065 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 4.47NLKK5 pKa = 8.87NTRR8 pKa = 11.84NAEE11 pKa = 3.75NRR13 pKa = 11.84TQNSRR18 pKa = 11.84NNAEE22 pKa = 3.87NRR24 pKa = 11.84TQNSRR29 pKa = 11.84NNAEE33 pKa = 4.41NRR35 pKa = 11.84TQDD38 pKa = 4.23SRR40 pKa = 11.84NNSQNTTGSNSTDD53 pKa = 2.99SRR55 pKa = 11.84NSTGARR61 pKa = 11.84NDD63 pKa = 3.9SKK65 pKa = 11.43DD66 pKa = 3.77CRR68 pKa = 11.84NNTQNSYY75 pKa = 10.88RR76 pKa = 11.84DD77 pKa = 3.25AAKK80 pKa = 10.45DD81 pKa = 3.43SKK83 pKa = 11.1QRR85 pKa = 3.43

Molecular weight: 9.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2438

0

2438

802576

35

2107

329.2

36.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.661 ± 0.05

1.388 ± 0.021

5.413 ± 0.039

7.456 ± 0.05

4.098 ± 0.032

7.208 ± 0.04

1.675 ± 0.019

7.939 ± 0.044

7.257 ± 0.045

8.984 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.048 ± 0.024

4.539 ± 0.029

3.319 ± 0.026

3.202 ± 0.026

4.029 ± 0.034

6.01 ± 0.042

5.384 ± 0.046

6.769 ± 0.041

0.788 ± 0.013

3.831 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski