Apis mellifera associated microvirus 17

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UU97|A0A3S8UU97_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 17 OX=2494744 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 10.44SVMLHH7 pKa = 6.56DD8 pKa = 4.8FSKK11 pKa = 10.56VPHH14 pKa = 6.31VEE16 pKa = 3.72IPRR19 pKa = 11.84SSFNRR24 pKa = 11.84SHH26 pKa = 6.45GCKK29 pKa = 7.67TTFNAGYY36 pKa = 9.15LVPVFVDD43 pKa = 3.93EE44 pKa = 4.54VLPGDD49 pKa = 3.98TFNLRR54 pKa = 11.84MNVFARR60 pKa = 11.84LATPLTPFMDD70 pKa = 3.48NMFLEE75 pKa = 4.65SFFFFVPYY83 pKa = 10.21RR84 pKa = 11.84LVWNNFQKK92 pKa = 10.9FMGEE96 pKa = 3.94QEE98 pKa = 4.65DD99 pKa = 4.39PGDD102 pKa = 3.8STDD105 pKa = 3.61YY106 pKa = 11.2LIPQVSAAVGGYY118 pKa = 9.18AQNSLFDD125 pKa = 3.78YY126 pKa = 10.76FGIPTQISGANPMLPNALPFRR147 pKa = 11.84AYY149 pKa = 11.07NLIWNEE155 pKa = 3.57WFRR158 pKa = 11.84DD159 pKa = 3.65QNLQNSATVNKK170 pKa = 10.26GDD172 pKa = 4.43GPDD175 pKa = 3.58SPVATTLLRR184 pKa = 11.84RR185 pKa = 11.84GKK187 pKa = 9.54RR188 pKa = 11.84HH189 pKa = 7.42DD190 pKa = 4.07YY191 pKa = 8.21FTSCLPWPQKK201 pKa = 10.67GDD203 pKa = 3.26AVTIPLVPEE212 pKa = 4.46SSTHH216 pKa = 5.05TIYY219 pKa = 10.69RR220 pKa = 11.84RR221 pKa = 11.84ANANVWQMYY230 pKa = 8.82VAGSEE235 pKa = 4.46TIAGTTGNLTNTATNGFLNSTGAGHH260 pKa = 7.13VSMNPGDD267 pKa = 4.2GLRR270 pKa = 11.84VDD272 pKa = 4.07VLPDD276 pKa = 3.36VTGGTINEE284 pKa = 4.06LRR286 pKa = 11.84QAFQIQKK293 pKa = 10.23LYY295 pKa = 10.9EE296 pKa = 4.3RR297 pKa = 11.84DD298 pKa = 3.34ARR300 pKa = 11.84GGTRR304 pKa = 11.84YY305 pKa = 8.58TEE307 pKa = 4.72IIRR310 pKa = 11.84SHH312 pKa = 6.66FGVVSPDD319 pKa = 3.07ARR321 pKa = 11.84LQRR324 pKa = 11.84PEE326 pKa = 3.87YY327 pKa = 10.14LGGGSTPVNINPVPQTSEE345 pKa = 4.16AGTTPQGNLAATGVAQGGGHH365 pKa = 6.62GFSKK369 pKa = 10.9SFTEE373 pKa = 3.84HH374 pKa = 6.6GIVIGMVSVRR384 pKa = 11.84ADD386 pKa = 3.34LNYY389 pKa = 10.12QQGLNRR395 pKa = 11.84MWSRR399 pKa = 11.84RR400 pKa = 11.84TKK402 pKa = 10.89LDD404 pKa = 3.97FYY406 pKa = 10.76WPTLSHH412 pKa = 7.04IGEE415 pKa = 3.93QAVLNKK421 pKa = 10.07EE422 pKa = 3.95IYY424 pKa = 9.63AQEE427 pKa = 3.82TAADD431 pKa = 4.11DD432 pKa = 4.08EE433 pKa = 4.89VFGYY437 pKa = 7.47QEE439 pKa = 4.05RR440 pKa = 11.84YY441 pKa = 9.49GEE443 pKa = 4.08YY444 pKa = 9.52RR445 pKa = 11.84YY446 pKa = 10.31KK447 pKa = 10.73PSIITGDD454 pKa = 3.51FRR456 pKa = 11.84SNASTSLDD464 pKa = 3.43TWHH467 pKa = 7.46LAQDD471 pKa = 4.44FASLPTLGPTFIEE484 pKa = 4.29EE485 pKa = 4.32NPPVARR491 pKa = 11.84VIATPTEE498 pKa = 3.74PHH500 pKa = 6.35FLFDD504 pKa = 3.65SYY506 pKa = 11.84MNLTCARR513 pKa = 11.84PMPVYY518 pKa = 10.19GVPGLVDD525 pKa = 3.28HH526 pKa = 7.13FF527 pKa = 4.86

Molecular weight: 58.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UU76|A0A3S8UU76_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 17 OX=2494744 PE=3 SV=1
MM1 pKa = 7.86AKK3 pKa = 9.99PLLAQRR9 pKa = 11.84VIMRR13 pKa = 11.84DD14 pKa = 3.5DD15 pKa = 3.18LTLRR19 pKa = 11.84SLRR22 pKa = 11.84DD23 pKa = 3.21FHH25 pKa = 6.48TVLLRR30 pKa = 11.84TLGLHH35 pKa = 6.37KK36 pKa = 10.75AGALTSEE43 pKa = 4.42GWPFKK48 pKa = 10.77RR49 pKa = 11.84KK50 pKa = 6.66ITRR53 pKa = 11.84RR54 pKa = 11.84SSLQIARR61 pKa = 11.84PYY63 pKa = 10.61VLIGAQCVRR72 pKa = 11.84ATRR75 pKa = 11.84ALMSLNPAADD85 pKa = 3.8RR86 pKa = 11.84PVVPLPTT93 pKa = 3.79

Molecular weight: 10.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1413

93

527

235.5

26.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.917 ± 1.507

1.132 ± 0.519

4.954 ± 0.306

5.662 ± 0.778

4.529 ± 0.735

7.289 ± 0.594

2.335 ± 0.405

4.317 ± 0.207

5.732 ± 1.468

7.643 ± 1.003

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.335 ± 0.479

4.6 ± 0.864

6.723 ± 0.753

4.105 ± 0.736

6.865 ± 1.043

6.44 ± 0.41

6.582 ± 0.677

5.591 ± 0.809

0.991 ± 0.32

3.255 ± 0.701
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski