Klebsiella phage ST13-OXA48phi12.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Felsduovirus; Klebsiella virus ST13OXA48phi12-1

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482J789|A0A482J789_9CAUD Portal protein OS=Klebsiella phage ST13-OXA48phi12.1 OX=2510464 PE=3 SV=1
MM1 pKa = 7.78LDD3 pKa = 3.17EE4 pKa = 5.33TEE6 pKa = 4.52AAWAACADD14 pKa = 3.83KK15 pKa = 11.02VDD17 pKa = 4.66TIIACQEE24 pKa = 3.92RR25 pKa = 11.84DD26 pKa = 3.52SEE28 pKa = 4.35QATVLTQRR36 pKa = 11.84SEE38 pKa = 3.79

Molecular weight: 4.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482J5E7|A0A482J5E7_9CAUD Regulatory protein CII OS=Klebsiella phage ST13-OXA48phi12.1 OX=2510464 PE=4 SV=1
MM1 pKa = 7.37NLHH4 pKa = 6.52DD5 pKa = 4.55RR6 pKa = 11.84LRR8 pKa = 11.84IVLAPARR15 pKa = 11.84QFWRR19 pKa = 11.84AFSYY23 pKa = 10.73LMQVHH28 pKa = 6.43EE29 pKa = 3.96NHH31 pKa = 4.89YY32 pKa = 8.78TKK34 pKa = 10.66RR35 pKa = 11.84AGVAGIRR42 pKa = 11.84ARR44 pKa = 11.84VQGAKK49 pKa = 10.05LAEE52 pKa = 4.43SKK54 pKa = 11.25GDD56 pKa = 3.41

Molecular weight: 6.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12239

38

875

244.8

27.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.715 ± 0.438

1.013 ± 0.139

6.12 ± 0.236

6.496 ± 0.386

3.513 ± 0.228

6.912 ± 0.378

1.699 ± 0.197

5.197 ± 0.229

5.147 ± 0.288

9.372 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.868 ± 0.195

4.314 ± 0.201

4.11 ± 0.181

4.428 ± 0.196

6.749 ± 0.396

5.932 ± 0.209

5.916 ± 0.328

6.251 ± 0.28

1.471 ± 0.133

2.778 ± 0.2
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski