Peromyscus maniculatus bairdii (Prairie deer mouse)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia;

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37544 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J0CSN3|A0A6J0CSN3_PERMB Isoform of A0A6I9MMH1 protein FAM111A-like isoform X2 OS=Peromyscus maniculatus bairdii OX=230844 GN=LOC102922038 PE=4 SV=1
MM1 pKa = 7.15GQLEE5 pKa = 4.44NHH7 pKa = 6.92DD8 pKa = 4.76MNTVTYY14 pKa = 10.44DD15 pKa = 3.33DD16 pKa = 3.81VRR18 pKa = 11.84IDD20 pKa = 3.79FTWEE24 pKa = 3.09EE25 pKa = 4.17WTLLDD30 pKa = 4.58PSQKK34 pKa = 10.51NLYY37 pKa = 9.1KK38 pKa = 10.73DD39 pKa = 3.71VMLEE43 pKa = 3.96TYY45 pKa = 10.71RR46 pKa = 11.84NLTSIGYY53 pKa = 8.66IWEE56 pKa = 3.93DD57 pKa = 3.39HH58 pKa = 6.73NIEE61 pKa = 4.7EE62 pKa = 4.78YY63 pKa = 9.87CQSSRR68 pKa = 11.84GHH70 pKa = 6.58EE71 pKa = 4.2STPVIPAQLVQDD83 pKa = 4.78DD84 pKa = 4.62DD85 pKa = 5.26ADD87 pKa = 3.54

Molecular weight: 10.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I9M169|A0A6I9M169_PERMB nuclear protein localization protein 4 homolog OS=Peromyscus maniculatus bairdii OX=230844 GN=Nploc4 PE=3 SV=1
MM1 pKa = 7.57SSHH4 pKa = 5.15KK5 pKa = 8.91TFRR8 pKa = 11.84IKK10 pKa = 10.64RR11 pKa = 11.84FLAKK15 pKa = 9.71KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21517

16027

37544

26604240

24

33992

708.6

78.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.878 ± 0.014

2.092 ± 0.011

4.919 ± 0.009

7.356 ± 0.023

3.439 ± 0.011

6.28 ± 0.017

2.562 ± 0.012

4.318 ± 0.016

6.005 ± 0.028

9.63 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.123 ± 0.009

3.526 ± 0.009

6.434 ± 0.02

4.855 ± 0.025

5.611 ± 0.011

8.556 ± 0.02

5.481 ± 0.018

6.165 ± 0.027

1.151 ± 0.004

2.613 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski