Bacillus methanolicus (strain MGA3 / ATCC 53907)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; Bacillus methanolicus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3271 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3E7S8|I3E7S8_BACMM Stage V sporulation protein AD OS=Bacillus methanolicus (strain MGA3 / ATCC 53907) OX=796606 GN=spoVAD PE=4 SV=1
MM1 pKa = 7.26PAIVGVVQVITIGSSSIFNIGDD23 pKa = 3.49VYY25 pKa = 11.41KK26 pKa = 11.08VMPVSTAKK34 pKa = 9.62TFSGAGSFNTGDD46 pKa = 3.23GLYY49 pKa = 10.45VYY51 pKa = 9.66NQQSSTNTYY60 pKa = 9.06DD61 pKa = 4.33TDD63 pKa = 5.75LLDD66 pKa = 3.97QGNFFNAA73 pKa = 3.65

Molecular weight: 7.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3DUK4|I3DUK4_BACMM UPF0349 protein BMMGA3_14185 OS=Bacillus methanolicus (strain MGA3 / ATCC 53907) OX=796606 GN=BMMGA3_14185 PE=3 SV=1
MM1 pKa = 7.39GKK3 pKa = 8.15STIDD7 pKa = 3.17LLRR10 pKa = 11.84RR11 pKa = 11.84VGIFEE16 pKa = 4.81GISYY20 pKa = 10.66LVLLFIAMPLKK31 pKa = 10.64YY32 pKa = 9.87FAGLPVFVLIFGMIHH47 pKa = 6.96GILFILFVLLLFLVWQRR64 pKa = 11.84HH65 pKa = 4.48RR66 pKa = 11.84WPLIRR71 pKa = 11.84VFGAFIASLLPFGTFVLDD89 pKa = 3.15ARR91 pKa = 11.84LRR93 pKa = 11.84KK94 pKa = 9.45EE95 pKa = 3.94YY96 pKa = 11.06

Molecular weight: 11.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3271

0

3271

916863

29

1520

280.3

31.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.843 ± 0.043

0.732 ± 0.011

4.834 ± 0.031

7.623 ± 0.058

4.679 ± 0.044

6.835 ± 0.046

2.083 ± 0.019

8.172 ± 0.043

7.761 ± 0.046

9.755 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.643 ± 0.021

4.467 ± 0.033

3.732 ± 0.029

3.513 ± 0.03

4.258 ± 0.033

5.791 ± 0.029

5.038 ± 0.029

6.775 ± 0.038

0.993 ± 0.017

3.473 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski