Rhizobium sp. YS-1r

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5595 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A095XRR0|A0A095XRR0_9RHIZ ABC transporter permease OS=Rhizobium sp. YS-1r OX=1532558 GN=JL39_02075 PE=3 SV=1
MM1 pKa = 7.73PNYY4 pKa = 9.84FVFGISLACYY14 pKa = 10.29LMAGTAGRR22 pKa = 11.84AADD25 pKa = 4.03VTPLLAQDD33 pKa = 4.0PNMVEE38 pKa = 4.76AQDD41 pKa = 3.18GWTFTFAPYY50 pKa = 10.16FWGTSISGDD59 pKa = 3.25TAQFSLPSVHH69 pKa = 6.9IDD71 pKa = 3.29ADD73 pKa = 3.76FGDD76 pKa = 4.62ILDD79 pKa = 4.77HH80 pKa = 7.51LDD82 pKa = 3.59FAAMAAGEE90 pKa = 3.95ARR92 pKa = 11.84YY93 pKa = 10.33DD94 pKa = 3.54RR95 pKa = 11.84FSIIGDD101 pKa = 3.27IEE103 pKa = 4.18YY104 pKa = 10.99AKK106 pKa = 10.69LSVGASTPLGILASDD121 pKa = 3.87VEE123 pKa = 4.83VATEE127 pKa = 4.05MFTGLVGAGYY137 pKa = 10.37SVLADD142 pKa = 3.19SSGYY146 pKa = 10.42LDD148 pKa = 3.25IVAGIKK154 pKa = 9.47VWSVDD159 pKa = 3.32TTISFSGGLLDD170 pKa = 4.11GVEE173 pKa = 4.56RR174 pKa = 11.84SDD176 pKa = 3.51SATWVDD182 pKa = 3.61GLAGIRR188 pKa = 11.84GRR190 pKa = 11.84YY191 pKa = 9.4SITPEE196 pKa = 3.79VYY198 pKa = 7.92ITGWGLVGGGGADD211 pKa = 4.18LDD213 pKa = 3.89WDD215 pKa = 3.77VALALGYY222 pKa = 10.52NFNEE226 pKa = 4.24RR227 pKa = 11.84LSVIAGYY234 pKa = 9.84RR235 pKa = 11.84AAGVDD240 pKa = 3.66YY241 pKa = 11.02SDD243 pKa = 4.83DD244 pKa = 3.64GFVFDD249 pKa = 5.75VVQQGPILSLALRR262 pKa = 11.84FF263 pKa = 3.85

Molecular weight: 27.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A095WTA8|A0A095WTA8_9RHIZ ABC transporter permease OS=Rhizobium sp. YS-1r OX=1532558 GN=JL39_03900 PE=4 SV=1
MM1 pKa = 7.88PNLINEE7 pKa = 4.32IVTGIVQSAMKK18 pKa = 10.04EE19 pKa = 4.02LLKK22 pKa = 10.59KK23 pKa = 9.97SHH25 pKa = 5.91GRR27 pKa = 11.84TSTKK31 pKa = 9.61RR32 pKa = 11.84KK33 pKa = 9.39KK34 pKa = 10.12RR35 pKa = 11.84QTRR38 pKa = 11.84SATSGRR44 pKa = 11.84FVTKK48 pKa = 8.43TTARR52 pKa = 11.84TAKK55 pKa = 9.42PAKK58 pKa = 9.17KK59 pKa = 9.17QVSRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84TAASRR70 pKa = 11.84SRR72 pKa = 11.84QRR74 pKa = 11.84NHH76 pKa = 5.71

Molecular weight: 8.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5595

0

5595

1746909

41

2482

312.2

34.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.619 ± 0.04

0.786 ± 0.01

5.594 ± 0.025

5.966 ± 0.029

4.046 ± 0.023

8.346 ± 0.031

2.016 ± 0.015

5.81 ± 0.023

3.725 ± 0.028

9.955 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.705 ± 0.015

2.815 ± 0.017

5.043 ± 0.024

2.975 ± 0.017

6.725 ± 0.032

5.608 ± 0.022

5.242 ± 0.021

7.344 ± 0.031

1.314 ± 0.014

2.365 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski