Streptococcus phage A25

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M5M629|A0A0M5M629_9CAUD Uncharacterized protein OS=Streptococcus phage A25 OX=1701850 GN=A25_21 PE=4 SV=1
MM1 pKa = 7.27QNNKK5 pKa = 8.99NFQEE9 pKa = 4.11VLLDD13 pKa = 4.13HH14 pKa = 6.99INAIKK19 pKa = 10.33DD20 pKa = 3.67LPIKK24 pKa = 10.66CRR26 pKa = 11.84LDD28 pKa = 3.44YY29 pKa = 11.27FNDD32 pKa = 3.51EE33 pKa = 4.31GDD35 pKa = 4.06DD36 pKa = 3.81LVINAIPGGKK46 pKa = 8.46IVKK49 pKa = 9.76EE50 pKa = 3.91YY51 pKa = 10.31MDD53 pKa = 3.71LTRR56 pKa = 11.84EE57 pKa = 3.66VSLPFEE63 pKa = 4.17IAVKK67 pKa = 10.5SKK69 pKa = 10.66NNRR72 pKa = 11.84LASDD76 pKa = 4.9IIWKK80 pKa = 10.26INGDD84 pKa = 3.51LSEE87 pKa = 5.09FDD89 pKa = 5.42LEE91 pKa = 5.17LLSTDD96 pKa = 3.71DD97 pKa = 4.05SYY99 pKa = 12.05HH100 pKa = 5.87FLSLEE105 pKa = 3.76VDD107 pKa = 3.26KK108 pKa = 11.33PGINGQDD115 pKa = 3.21EE116 pKa = 4.32QGYY119 pKa = 9.4FIYY122 pKa = 10.15TLQVQALIEE131 pKa = 4.04IEE133 pKa = 3.93KK134 pKa = 10.9GEE136 pKa = 4.03EE137 pKa = 3.83HH138 pKa = 6.9GG139 pKa = 4.75

Molecular weight: 15.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M5M1N5|A0A0M5M1N5_9CAUD Putative capsid protein OS=Streptococcus phage A25 OX=1701850 GN=A25_34 PE=4 SV=1
MM1 pKa = 7.68LGQEE5 pKa = 4.16IDD7 pKa = 3.27KK8 pKa = 10.59LQNKK12 pKa = 8.71VIALTRR18 pKa = 11.84EE19 pKa = 3.51NDD21 pKa = 3.3RR22 pKa = 11.84PKK24 pKa = 10.95AEE26 pKa = 3.57KK27 pKa = 9.22WQLKK31 pKa = 8.1RR32 pKa = 11.84RR33 pKa = 11.84KK34 pKa = 9.5RR35 pKa = 11.84KK36 pKa = 9.37

Molecular weight: 4.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

10639

36

1235

231.3

25.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.754 ± 1.102

0.479 ± 0.092

6.42 ± 0.426

6.138 ± 0.57

4.145 ± 0.221

7.04 ± 0.73

1.485 ± 0.181

6.824 ± 0.215

8.234 ± 0.52

7.83 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.698 ± 0.187

5.593 ± 0.435

2.754 ± 0.241

3.854 ± 0.234

3.92 ± 0.286

6.504 ± 0.477

6.617 ± 0.384

6.514 ± 0.284

1.184 ± 0.179

4.014 ± 0.411
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski