Bacillus phage TsarBomba

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Tsarbombavirus; Bacillus virus TsarBomba

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 234 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2D063|A0A0K2D063_9CAUD Uncharacterized protein OS=Bacillus phage TsarBomba OX=1690456 GN=TSARBOMBA_2 PE=4 SV=1
MM1 pKa = 7.22GKK3 pKa = 9.7VYY5 pKa = 9.68TVAVPVVAKK14 pKa = 10.58VLVEE18 pKa = 3.75VSGVNSEE25 pKa = 4.02QEE27 pKa = 4.43AIEE30 pKa = 4.25KK31 pKa = 10.45ALAVDD36 pKa = 3.47MTLNPSCSDD45 pKa = 3.16TTVDD49 pKa = 3.74LEE51 pKa = 3.99EE52 pKa = 4.7WDD54 pKa = 3.42THH56 pKa = 5.23KK57 pKa = 10.92QVVQGNVYY65 pKa = 9.53YY66 pKa = 10.98GCINEE71 pKa = 4.58PYY73 pKa = 9.96VAAEE77 pKa = 4.25DD78 pKa = 4.15EE79 pKa = 4.51EE80 pKa = 4.54

Molecular weight: 8.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2CZX1|A0A0K2CZX1_9CAUD Uncharacterized protein OS=Bacillus phage TsarBomba OX=1690456 GN=TSARBOMBA_244 PE=4 SV=1
MM1 pKa = 7.14SRR3 pKa = 11.84RR4 pKa = 11.84VRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84AVSRR12 pKa = 11.84CKK14 pKa = 10.65GSNSEE19 pKa = 4.13IKK21 pKa = 9.38TYY23 pKa = 8.73WRR25 pKa = 11.84NRR27 pKa = 11.84VRR29 pKa = 11.84DD30 pKa = 3.49NATNLTPEE38 pKa = 3.96YY39 pKa = 9.06MLSYY43 pKa = 10.3RR44 pKa = 11.84NRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84QLFNEE53 pKa = 4.2LHH55 pKa = 6.45HH56 pKa = 7.8DD57 pKa = 3.72YY58 pKa = 10.08TQVLNKK64 pKa = 9.87ARR66 pKa = 11.84AEE68 pKa = 4.07GVTINLNGTVTGRR81 pKa = 11.84FKK83 pKa = 11.27SNEE86 pKa = 3.82LNITGTPKK94 pKa = 9.84TLGGKK99 pKa = 8.08DD100 pKa = 3.3HH101 pKa = 6.84EE102 pKa = 4.61

Molecular weight: 11.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

234

0

234

47836

34

1451

204.4

23.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.554 ± 0.21

0.786 ± 0.068

5.899 ± 0.122

7.768 ± 0.306

3.92 ± 0.111

6.52 ± 0.293

1.865 ± 0.096

6.512 ± 0.148

7.963 ± 0.206

8.046 ± 0.175

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.868 ± 0.098

5.37 ± 0.145

3.322 ± 0.165

3.876 ± 0.137

4.507 ± 0.13

5.602 ± 0.172

6.177 ± 0.238

6.974 ± 0.162

1.125 ± 0.072

4.346 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski