Gemella sp. WT2a

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillales incertae sedis; Bacillales Family XI. Incertae Sedis; Gemella; unclassified Gemella

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1666 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y9H7K9|A0A4Y9H7K9_9BACL MFS transporter (Fragment) OS=Gemella sp. WT2a OX=2558281 GN=E4T67_07890 PE=4 SV=1
EEE2 pKa = 4.88NTSFKKK8 pKa = 10.89YYY10 pKa = 9.78EE11 pKa = 4.21QDDD14 pKa = 3.66TKKK17 pKa = 9.11TDDD20 pKa = 3.08YYY22 pKa = 11.26YY23 pKa = 10.22NPEEE27 pKa = 3.6DD28 pKa = 3.72NYYY31 pKa = 10.06DDD33 pKa = 3.34TNQFSNAITYYY44 pKa = 10.08DDD46 pKa = 3.44TATIDDD52 pKa = 3.69SNTSKKK58 pKa = 9.97YYY60 pKa = 9.23VVVDDD65 pKa = 3.81HHH67 pKa = 6.51DDD69 pKa = 3.31SGEEE73 pKa = 4.28VRR75 pKa = 11.84TRR77 pKa = 11.84VTHHH81 pKa = 6.98NTDDD85 pKa = 3.16YY86 pKa = 11.25YY87 pKa = 11.5NQNTYYY93 pKa = 9.82YY94 pKa = 9.36DDD96 pKa = 3.43EEE98 pKa = 4.35IVKKK102 pKa = 10.25GSGSGDDD109 pKa = 3.37DDD111 pKa = 4.26DD112 pKa = 6.2DD113 pKa = 5.1DDD115 pKa = 4.35DDD117 pKa = 4.51DDD119 pKa = 4.02DDD121 pKa = 4.0DDD123 pKa = 4.37DDD125 pKa = 4.15DDD127 pKa = 3.85DDD129 pKa = 4.78DDD131 pKa = 4.23DDD133 pKa = 3.61D

Molecular weight: 14.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y9HCP6|A0A4Y9HCP6_9BACL Cytochrome O ubiquinol oxidase OS=Gemella sp. WT2a OX=2558281 GN=E4T67_05460 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.91VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.08NGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1666

0

1666

436377

20

1666

261.9

29.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.265 ± 0.082

0.597 ± 0.019

5.544 ± 0.052

7.626 ± 0.082

4.465 ± 0.054

5.911 ± 0.065

1.516 ± 0.025

9.08 ± 0.076

8.879 ± 0.08

9.667 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.367 ± 0.034

5.753 ± 0.06

2.675 ± 0.037

2.767 ± 0.032

3.613 ± 0.045

6.534 ± 0.056

5.219 ± 0.064

6.589 ± 0.054

0.643 ± 0.021

4.291 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski