Rugosibacter aromaticivorans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Rhodocyclaceae; Rugosibacter

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2443 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5JPR6|A0A0C5JPR6_9RHOO ATP synthase subunit beta OS=Rugosibacter aromaticivorans OX=1565605 GN=atpD PE=3 SV=1
MM1 pKa = 7.43ATTEE5 pKa = 3.81QPYY8 pKa = 10.67RR9 pKa = 11.84KK10 pKa = 9.07LLCLICGLIYY20 pKa = 10.51DD21 pKa = 4.7EE22 pKa = 4.91SEE24 pKa = 3.61GWPEE28 pKa = 3.91DD29 pKa = 4.13GIPAGTRR36 pKa = 11.84WEE38 pKa = 4.19DD39 pKa = 3.48VPPNWTCPDD48 pKa = 3.36CNARR52 pKa = 11.84KK53 pKa = 9.9DD54 pKa = 3.76DD55 pKa = 4.0FEE57 pKa = 4.28MVEE60 pKa = 3.98AA61 pKa = 5.12

Molecular weight: 6.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5JLK3|A0A0C5JLK3_9RHOO 2Fe-2S ferredoxin OS=Rugosibacter aromaticivorans OX=1565605 GN=PG1C_06870 PE=4 SV=1
MM1 pKa = 7.91PKK3 pKa = 9.63MKK5 pKa = 9.78TKK7 pKa = 10.62SGAKK11 pKa = 9.26KK12 pKa = 10.08RR13 pKa = 11.84FIVRR17 pKa = 11.84ASGSIKK23 pKa = 10.12RR24 pKa = 11.84SQAFKK29 pKa = 10.88RR30 pKa = 11.84HH31 pKa = 5.55ILTKK35 pKa = 9.74KK36 pKa = 3.98TTKK39 pKa = 9.52TKK41 pKa = 9.63RR42 pKa = 11.84QLRR45 pKa = 11.84GMLTVHH51 pKa = 6.98ASNVKK56 pKa = 9.38SVRR59 pKa = 11.84AMLPNAA65 pKa = 4.42

Molecular weight: 7.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2443

0

2443

758906

27

2953

310.6

34.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.494 ± 0.064

0.996 ± 0.016

5.298 ± 0.036

5.578 ± 0.044

3.849 ± 0.03

7.672 ± 0.044

2.448 ± 0.026

5.386 ± 0.037

3.957 ± 0.046

10.75 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.519 ± 0.021

3.044 ± 0.028

4.844 ± 0.034

3.854 ± 0.029

6.455 ± 0.043

5.374 ± 0.033

5.407 ± 0.032

7.223 ± 0.044

1.422 ± 0.023

2.43 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski