Clostridium magnum DSM 2767

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium magnum

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6083 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A161X586|A0A161X586_9CLOT Putative sensory transducer protein YfmS OS=Clostridium magnum DSM 2767 OX=1121326 GN=yfmS_8 PE=4 SV=1
MM1 pKa = 7.86SYY3 pKa = 11.1QDD5 pKa = 4.07LTTLQLDD12 pKa = 3.75ALSEE16 pKa = 4.17VGNIGAGNAATALSQLINKK35 pKa = 9.11KK36 pKa = 9.72IDD38 pKa = 3.38MTVPAINIVPFDD50 pKa = 5.62DD51 pKa = 3.66IFSSIGGDD59 pKa = 3.44EE60 pKa = 4.08IVVGVIVRR68 pKa = 11.84VLGEE72 pKa = 3.86IPGNILFVFEE82 pKa = 5.59KK83 pKa = 10.21DD84 pKa = 2.98TALNLVEE91 pKa = 5.18ILTGLQEE98 pKa = 4.48EE99 pKa = 4.93YY100 pKa = 10.71LSDD103 pKa = 3.32MGNSVICEE111 pKa = 3.65IGNIISSSYY120 pKa = 9.56MNAIAKK126 pKa = 7.59FTGLTVMPSVPAVTYY141 pKa = 11.26DD142 pKa = 3.26MLGAILSTTFIEE154 pKa = 4.93SGQFDD159 pKa = 5.31DD160 pKa = 4.38YY161 pKa = 12.0VLDD164 pKa = 3.75IEE166 pKa = 5.09TLFIQNNEE174 pKa = 4.21EE175 pKa = 4.09ISGHH179 pKa = 5.84FYY181 pKa = 10.11YY182 pKa = 11.17VPMPGSLEE190 pKa = 4.59KK191 pKa = 10.38ILSTLGVV198 pKa = 3.44

Molecular weight: 21.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A162TL56|A0A162TL56_9CLOT Histidine kinase OS=Clostridium magnum DSM 2767 OX=1121326 GN=ypdA_2 PE=4 SV=1
MM1 pKa = 7.72LKK3 pKa = 10.4RR4 pKa = 11.84SVKK7 pKa = 10.47LIASLAIVVSSLGAIGVQPRR27 pKa = 11.84YY28 pKa = 9.91AVRR31 pKa = 11.84PDD33 pKa = 3.34RR34 pKa = 11.84AHH36 pKa = 6.66ILAGGTPVKK45 pKa = 10.85VRR47 pKa = 11.84FTKK50 pKa = 10.63SPCIEE55 pKa = 3.83SWRR58 pKa = 11.84CCGNVTFF65 pKa = 5.62

Molecular weight: 7.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6083

0

6083

1781211

29

4851

292.8

32.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.112 ± 0.039

1.275 ± 0.014

5.414 ± 0.026

7.18 ± 0.04

4.213 ± 0.026

6.543 ± 0.034

1.412 ± 0.015

9.538 ± 0.035

8.743 ± 0.03

8.917 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.656 ± 0.015

5.959 ± 0.031

2.924 ± 0.019

2.591 ± 0.018

3.432 ± 0.023

6.55 ± 0.034

5.143 ± 0.035

6.751 ± 0.032

0.717 ± 0.01

3.931 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski