Desulfopila aestuarii DSM 18488

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobulbaceae; Desulfopila; Desulfopila aestuarii

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M7XVQ2|A0A1M7XVQ2_9DELT Uncharacterized protein OS=Desulfopila aestuarii DSM 18488 OX=1121416 GN=SAMN02745220_00117 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.71PKK4 pKa = 10.36SIVGAIFAATFSFATLTQAADD25 pKa = 3.56VSVLDD30 pKa = 4.96LLTNDD35 pKa = 4.21LVANQSTGLLYY46 pKa = 10.91ASVPGNAGGGYY57 pKa = 9.04GNSVVEE63 pKa = 4.16INPFQGTITQVLPVGSEE80 pKa = 3.86PSVLSLSDD88 pKa = 4.56DD89 pKa = 3.48GTTLYY94 pKa = 11.18VSLNGSNSIRR104 pKa = 11.84KK105 pKa = 8.8IDD107 pKa = 3.86LQSMTMEE114 pKa = 4.36QPFSLGSDD122 pKa = 3.68DD123 pKa = 5.11YY124 pKa = 11.62YY125 pKa = 11.92VSANN129 pKa = 3.38

Molecular weight: 13.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M7Y2W2|A0A1M7Y2W2_9DELT 1 4-dihydroxy-2-naphthoate octaprenyltransferase OS=Desulfopila aestuarii DSM 18488 OX=1121416 GN=menA PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLKK12 pKa = 10.2RR13 pKa = 11.84KK14 pKa = 7.57RR15 pKa = 11.84THH17 pKa = 5.9GFRR20 pKa = 11.84ARR22 pKa = 11.84MATRR26 pKa = 11.84AGRR29 pKa = 11.84AVIKK33 pKa = 10.33ARR35 pKa = 11.84RR36 pKa = 11.84AHH38 pKa = 5.03GRR40 pKa = 11.84KK41 pKa = 9.3RR42 pKa = 11.84LSAA45 pKa = 4.01

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5269

0

5269

1731742

29

2079

328.7

36.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.41 ± 0.036

1.302 ± 0.015

5.279 ± 0.024

6.566 ± 0.03

4.147 ± 0.02

7.461 ± 0.032

2.119 ± 0.016

6.827 ± 0.028

5.148 ± 0.037

10.312 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.783 ± 0.017

3.701 ± 0.022

4.243 ± 0.022

3.711 ± 0.02

5.411 ± 0.026

6.145 ± 0.029

5.501 ± 0.024

6.96 ± 0.03

1.053 ± 0.013

2.922 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski