Grimontia hollisae CIP 101886

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Grimontia; Grimontia hollisae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3553 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D0IBX1|D0IBX1_GRIHO Putative 2-succinyl-6-hydroxy-2 4-cyclohexadiene-1-carboxylate synthase OS=Grimontia hollisae CIP 101886 OX=675812 GN=menH PE=3 SV=1
MM1 pKa = 7.62AVPALLAAGAAQASVNLYY19 pKa = 10.33DD20 pKa = 4.36ADD22 pKa = 3.91GVTVDD27 pKa = 4.23LSGAAEE33 pKa = 3.66IQYY36 pKa = 10.5FKK38 pKa = 10.81DD39 pKa = 3.49YY40 pKa = 10.59STTEE44 pKa = 3.84DD45 pKa = 3.31AYY47 pKa = 11.58LRR49 pKa = 11.84IDD51 pKa = 4.86DD52 pKa = 5.25ADD54 pKa = 4.18LLLTTSIEE62 pKa = 4.31VADD65 pKa = 4.5GLNAVAGMGFKK76 pKa = 11.0YY77 pKa = 10.27EE78 pKa = 4.2DD79 pKa = 3.36QFATGVTVSNDD90 pKa = 2.76NGTVKK95 pKa = 10.57NDD97 pKa = 3.26EE98 pKa = 4.74LYY100 pKa = 11.17VGLGGNFGTVTFGRR114 pKa = 11.84QLLLSDD120 pKa = 4.6DD121 pKa = 4.39AGNQKK126 pKa = 10.56DD127 pKa = 3.88YY128 pKa = 11.43EE129 pKa = 4.25LGYY132 pKa = 10.01EE133 pKa = 4.2QIDD136 pKa = 3.64FVQTEE141 pKa = 4.65GGQVIKK147 pKa = 10.0WVYY150 pKa = 11.45DD151 pKa = 3.05NGTFYY156 pKa = 11.22AGANVDD162 pKa = 3.87LDD164 pKa = 4.29TNKK167 pKa = 10.36SDD169 pKa = 3.56VTDD172 pKa = 3.54GRR174 pKa = 11.84QIIGGRR180 pKa = 11.84IGARR184 pKa = 11.84YY185 pKa = 9.04EE186 pKa = 4.05GLDD189 pKa = 3.3GRR191 pKa = 11.84IYY193 pKa = 10.44FYY195 pKa = 11.07DD196 pKa = 4.43GEE198 pKa = 4.57DD199 pKa = 3.74VDD201 pKa = 5.74GEE203 pKa = 4.42DD204 pKa = 3.18IKK206 pKa = 11.53GFNAEE211 pKa = 4.67LDD213 pKa = 3.44WTINDD218 pKa = 3.66QFDD221 pKa = 3.54LALSYY226 pKa = 11.08GQLDD230 pKa = 4.24YY231 pKa = 11.35EE232 pKa = 4.37LHH234 pKa = 6.07TNNAVGDD241 pKa = 4.01EE242 pKa = 4.04VDD244 pKa = 3.56VFGISAGYY252 pKa = 8.33QASEE256 pKa = 4.22KK257 pKa = 10.56LVFAIGYY264 pKa = 9.29DD265 pKa = 3.73YY266 pKa = 11.28LDD268 pKa = 3.91SKK270 pKa = 11.58GKK272 pKa = 10.52GGNLDD277 pKa = 3.52GDD279 pKa = 3.96ADD281 pKa = 4.23SIYY284 pKa = 11.47ANATYY289 pKa = 10.61KK290 pKa = 10.57LHH292 pKa = 6.39SNAMVYY298 pKa = 10.88AEE300 pKa = 4.53VGVADD305 pKa = 4.12GKK307 pKa = 11.01VGNVDD312 pKa = 2.76IDD314 pKa = 3.57TDD316 pKa = 3.43TGYY319 pKa = 11.56VLGMEE324 pKa = 4.63VKK326 pKa = 10.58FF327 pKa = 4.23

Molecular weight: 35.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D0I2Z5|D0I2Z5_GRIHO Beta sliding clamp OS=Grimontia hollisae CIP 101886 OX=675812 GN=VHA_000108 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38NGRR28 pKa = 11.84KK29 pKa = 9.44VIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.33GRR39 pKa = 11.84SKK41 pKa = 10.8LSKK44 pKa = 10.52

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3553

0

3553

1131702

37

5261

318.5

35.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.267 ± 0.048

1.033 ± 0.015

5.688 ± 0.041

6.295 ± 0.041

4.006 ± 0.029

7.243 ± 0.043

2.232 ± 0.022

6.01 ± 0.034

4.981 ± 0.033

10.263 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.778 ± 0.023

4.018 ± 0.032

4.151 ± 0.028

4.066 ± 0.03

5.021 ± 0.042

6.102 ± 0.028

5.433 ± 0.046

7.338 ± 0.044

1.228 ± 0.016

2.849 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski