Leifsonia aquatica ATCC 14665

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leifsonia; Leifsonia aquatica

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4862 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U2RQN2|U2RQN2_LEIAQ CDP-alcohol phosphatidyltransferase OS=Leifsonia aquatica ATCC 14665 OX=1358026 GN=N136_02767 PE=4 SV=1
VV1 pKa = 7.03TITGTGFTGTTGVTFGGTPATGVTVVDD28 pKa = 4.29DD29 pKa = 3.86TTITATTPAHH39 pKa = 6.47APGAVDD45 pKa = 4.08VVVTTPAGPSEE56 pKa = 4.5PGDD59 pKa = 3.58FTFDD63 pKa = 3.16AVPAITSVAPASGPEE78 pKa = 3.92TGGTDD83 pKa = 3.29VTITGTGFTGATGVTFGGTAGTAFTVVNDD112 pKa = 3.67TTITVTTPAHH122 pKa = 6.14LPGAVDD128 pKa = 3.89VVVQHH133 pKa = 6.69PNGDD137 pKa = 3.85SQPGDD142 pKa = 3.41FTFLQLPAITSLAPTSGPQTGGTAVTITGTGFTGATGVTFGGTAGTAFTVVNDD195 pKa = 3.67TTITVTTPAHH205 pKa = 6.13APGGVDD211 pKa = 4.9VIVQHH216 pKa = 6.72PNGDD220 pKa = 3.86SQPGDD225 pKa = 3.49FTFLPVPAITSVAPANGPQTGGTAVTITGTGFTGATGVTFGGTPGTGFTVVNDD278 pKa = 3.62TTITVTTPAHH288 pKa = 5.62TPGGVDD294 pKa = 4.66VIVQHH299 pKa = 6.72PNGDD303 pKa = 3.86SQPGDD308 pKa = 3.44FTFDD312 pKa = 3.71PVPNAPVISGLDD324 pKa = 3.56PDD326 pKa = 4.69HH327 pKa = 6.93GPFLGGTVVTVSGTGFTGATSVTVDD352 pKa = 3.36GVDD355 pKa = 3.39VPFTVVNDD363 pKa = 3.75TTITLTTPPHH373 pKa = 5.52TAGAVPIVVTTPVGPSPSATFTYY396 pKa = 10.74DD397 pKa = 2.63PGTTVDD403 pKa = 4.7GVDD406 pKa = 4.06PGHH409 pKa = 6.6GPQAGGTTVTITGGCFTGATGVLFGTTPATSFTVVDD445 pKa = 4.53DD446 pKa = 3.95ATITAVAPAGTGTVDD461 pKa = 3.15VTVTGGVEE469 pKa = 4.08CGNGVLTGGYY479 pKa = 9.52RR480 pKa = 11.84YY481 pKa = 9.77DD482 pKa = 3.59VPLPADD488 pKa = 4.34PGTGTTGSTSTGSGLADD505 pKa = 3.25TGSTSGGAALLGGLAALLVACGALILIRR533 pKa = 11.84RR534 pKa = 11.84TRR536 pKa = 11.84RR537 pKa = 11.84AA538 pKa = 3.03

Molecular weight: 51.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U2RJS3|U2RJS3_LEIAQ 2 5-diketo-D-gluconic acid reductase A OS=Leifsonia aquatica ATCC 14665 OX=1358026 GN=N136_04532 PE=4 SV=1
MM1 pKa = 6.95TGRR4 pKa = 11.84RR5 pKa = 11.84GVPATPSGLVRR16 pKa = 11.84QRR18 pKa = 11.84GSSIPAAMIAIATAATTGRR37 pKa = 11.84APRR40 pKa = 11.84AA41 pKa = 3.47

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4862

0

4862

1291825

29

2132

265.7

28.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.504 ± 0.049

0.494 ± 0.009

6.062 ± 0.029

5.365 ± 0.038

3.215 ± 0.024

9.079 ± 0.033

1.88 ± 0.017

4.51 ± 0.03

2.043 ± 0.034

10.232 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.676 ± 0.015

2.027 ± 0.022

5.394 ± 0.031

2.803 ± 0.023

6.998 ± 0.045

5.778 ± 0.031

6.342 ± 0.042

9.046 ± 0.036

1.498 ± 0.018

2.053 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski