Proteome-pI 2.0 - Proteome Isoelectric Point Database

Ophiostoma mitovirus 4

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Howeltoviricetes; Cryppavirales; Mitoviridae; Mitovirus

Average proteome isoelectric point is 8.79

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|Q9WJ22|Q9WJ22_9VIRU RNA-dependent RNA polymerase  putative OS=Ophiostoma mitovirus 4 OX=88387 GN=pol4 PE=2 SV=1

MM1 pKa = 7.46KK2 pKa = 10.34RR3 pKa = 11.84NNLQIIIIKK12 pKa = 10.26RR13 pKa = 11.84LILHH17 pKa = 6.48IFKK20 pKa = 10.54INLSVEE26 pKa = 3.47IDD28 pKa = 3.38KK29 pKa = 11.06FLGFINHH36 pKa = 5.91LRR38 pKa = 11.84KK39 pKa = 10.32SNGLLYY45 pKa = 9.64TIKK48 pKa = 10.68YY49 pKa = 7.33MKK51 pKa = 10.02AAKK54 pKa = 10.03LHH56 pKa = 5.11ITRR59 pKa = 11.84YY60 pKa = 8.33MCGKK64 pKa = 9.14PLYY67 pKa = 10.63SNNEE71 pKa = 3.47NVALDD76 pKa = 3.52KK77 pKa = 11.18TGFPLRR83 pKa = 11.84FWYY86 pKa = 10.26LKK88 pKa = 10.62RR89 pKa = 11.84LVNDD93 pKa = 3.26NPRR96 pKa = 11.84ALLTLLTYY104 pKa = 7.99TRR106 pKa = 11.84RR107 pKa = 11.84IVPNKK112 pKa = 9.83SEE114 pKa = 4.06SKK116 pKa = 10.54ARR118 pKa = 11.84IVKK121 pKa = 10.48LSTITDD127 pKa = 3.97PYY129 pKa = 9.27KK130 pKa = 10.86GKK132 pKa = 10.9VYY134 pKa = 9.45TIPKK138 pKa = 9.12WFILDD143 pKa = 4.68FISKK147 pKa = 10.5YY148 pKa = 10.21NLSSTKK154 pKa = 10.11PIYY157 pKa = 9.91TDD159 pKa = 3.03NDD161 pKa = 3.58HH162 pKa = 6.38YY163 pKa = 11.56LSIKK167 pKa = 10.25GSPNGKK173 pKa = 10.05ASMSSLYY180 pKa = 10.75SIISFNSSNIRR191 pKa = 11.84YY192 pKa = 8.62LFNIVGDD199 pKa = 3.95YY200 pKa = 10.94QLVLNKK206 pKa = 9.73FYY208 pKa = 11.04QDD210 pKa = 3.42LSQFYY215 pKa = 8.96TKK217 pKa = 10.69YY218 pKa = 10.41INRR221 pKa = 11.84DD222 pKa = 3.03KK223 pKa = 11.39LGLGKK228 pKa = 10.47LSIVHH233 pKa = 6.88DD234 pKa = 4.31PEE236 pKa = 4.72LKK238 pKa = 9.94EE239 pKa = 4.09RR240 pKa = 11.84VIAMVDD246 pKa = 3.38YY247 pKa = 7.55TTQFALRR254 pKa = 11.84PIHH257 pKa = 7.01NILLNNLSKK266 pKa = 10.94LPCDD270 pKa = 3.22RR271 pKa = 11.84TFTQDD276 pKa = 5.3PFHH279 pKa = 7.03KK280 pKa = 10.3WNDD283 pKa = 3.45DD284 pKa = 3.18HH285 pKa = 8.39KK286 pKa = 10.77EE287 pKa = 4.1RR288 pKa = 11.84YY289 pKa = 9.21HH290 pKa = 8.71SLDD293 pKa = 3.16LSAATDD299 pKa = 3.8RR300 pKa = 11.84FPIFLQQKK308 pKa = 9.61LISLIFNDD316 pKa = 4.47YY317 pKa = 11.18EE318 pKa = 4.63FGKK321 pKa = 10.07NWRR324 pKa = 11.84NLLVDD329 pKa = 4.32RR330 pKa = 11.84NYY332 pKa = 11.02DD333 pKa = 3.55YY334 pKa = 11.47QGISYY339 pKa = 9.07RR340 pKa = 11.84YY341 pKa = 9.63SVGQPMGAYY350 pKa = 8.28TSWAAFTLTHH360 pKa = 6.41HH361 pKa = 6.96LVVHH365 pKa = 6.47WAAEE369 pKa = 3.99LAGLKK374 pKa = 10.05NFKK377 pKa = 10.48DD378 pKa = 4.22YY379 pKa = 10.95IILGDD384 pKa = 5.07DD385 pKa = 3.69IVIKK389 pKa = 10.38NNKK392 pKa = 7.75VAQIYY397 pKa = 9.81INLMTKK403 pKa = 9.49WGVDD407 pKa = 2.8ISLSKK412 pKa = 9.15THH414 pKa = 6.07VSYY417 pKa = 8.85DD418 pKa = 3.15TYY420 pKa = 11.38EE421 pKa = 3.85FAKK424 pKa = 10.34RR425 pKa = 11.84WIKK428 pKa = 10.26NGKK431 pKa = 8.17EE432 pKa = 3.41ISGISLKK439 pKa = 11.08GILTNIRR446 pKa = 11.84HH447 pKa = 4.95IHH449 pKa = 4.58VVYY452 pKa = 10.46MNIFTYY458 pKa = 9.72LQRR461 pKa = 11.84IPSLNVDD468 pKa = 3.18ILTCVGKK475 pKa = 10.75LYY477 pKa = 10.79GYY479 pKa = 10.64LLIRR483 pKa = 11.84NRR485 pKa = 11.84IKK487 pKa = 10.84SPNTIKK493 pKa = 10.64RR494 pKa = 11.84SLYY497 pKa = 10.3DD498 pKa = 3.01FHH500 pKa = 8.96HH501 pKa = 7.1SIRR504 pKa = 11.84YY505 pKa = 9.15SFGLLNYY512 pKa = 10.15EE513 pKa = 4.34EE514 pKa = 4.22IRR516 pKa = 11.84NYY518 pKa = 10.6LHH520 pKa = 7.18NKK522 pKa = 9.52FPFDD526 pKa = 4.46NYY528 pKa = 9.8YY529 pKa = 10.73AWPEE533 pKa = 3.93RR534 pKa = 11.84LVHH537 pKa = 6.53SKK539 pKa = 10.86LNEE542 pKa = 3.73IFKK545 pKa = 11.23LEE547 pKa = 4.06MVEE550 pKa = 4.31SAKK553 pKa = 10.88SFSKK557 pKa = 11.09DD558 pKa = 3.23FMNQSTMLINTVTDD572 pKa = 3.53NEE574 pKa = 4.98IMVQWPLYY582 pKa = 9.71KK583 pKa = 10.69GFMNHH588 pKa = 5.99IEE590 pKa = 4.08KK591 pKa = 10.79LKK593 pKa = 10.8DD594 pKa = 3.63YY595 pKa = 10.59IKK597 pKa = 10.8SKK599 pKa = 10.68QNQHH603 pKa = 7.29DD604 pKa = 3.94IDD606 pKa = 5.4LLDD609 pKa = 3.89LMQNLRR615 pKa = 11.84FQNLDD620 pKa = 3.73SIVKK624 pKa = 10.07KK625 pKa = 10.62LRR627 pKa = 11.84NSYY630 pKa = 9.95TNLIMLDD637 pKa = 4.1KK638 pKa = 10.19FWKK641 pKa = 9.58SAFNRR646 pKa = 11.84EE647 pKa = 3.94YY648 pKa = 10.81RR649 pKa = 11.84DD650 pKa = 3.59LEE652 pKa = 4.2RR653 pKa = 11.84EE654 pKa = 4.17SILTIEE660 pKa = 4.26KK661 pKa = 10.24QEE663 pKa = 4.42SNMMSRR669 pKa = 11.84IWDD672 pKa = 3.39MALSYY677 pKa = 8.91RR678 pKa = 11.84TSPMSYY684 pKa = 8.62STLTFEE690 pKa = 4.75TDD692 pKa = 2.9SEE694 pKa = 5.25FYY696 pKa = 10.31MMPSIWDD703 pKa = 3.62MASSSTTGPKK713 pKa = 10.29PFTTATFKK721 pKa = 10.83TDD723 pKa = 3.58FVGLSTFDD731 pKa = 4.49DD732 pKa = 4.2KK733 pKa = 11.56LLKK736 pKa = 10.66DD737 pKa = 4.24LEE739 pKa = 4.22NLKK742 pKa = 10.03IDD744 pKa = 3.12ITLRR748 pKa = 11.84TGKK751 pKa = 8.58YY752 pKa = 7.22TNKK755 pKa = 8.67TQPLEE760 pKa = 4.4SKK762 pKa = 9.78IHH764 pKa = 4.41QHH766 pKa = 6.58PIEE769 pKa = 5.01CIEE772 pKa = 4.51DD773 pKa = 3.45NKK775 pKa = 10.46VPNFNNIKK783 pKa = 10.42

Molecular weight: 92.25 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|Q9WJ22|Q9WJ22_9VIRU RNA-dependent RNA polymerase  putative OS=Ophiostoma mitovirus 4 OX=88387 GN=pol4 PE=2 SV=1

MM1 pKa = 7.46KK2 pKa = 10.34RR3 pKa = 11.84NNLQIIIIKK12 pKa = 10.26RR13 pKa = 11.84LILHH17 pKa = 6.48IFKK20 pKa = 10.54INLSVEE26 pKa = 3.47IDD28 pKa = 3.38KK29 pKa = 11.06FLGFINHH36 pKa = 5.91LRR38 pKa = 11.84KK39 pKa = 10.32SNGLLYY45 pKa = 9.64TIKK48 pKa = 10.68YY49 pKa = 7.33MKK51 pKa = 10.02AAKK54 pKa = 10.03LHH56 pKa = 5.11ITRR59 pKa = 11.84YY60 pKa = 8.33MCGKK64 pKa = 9.14PLYY67 pKa = 10.63SNNEE71 pKa = 3.47NVALDD76 pKa = 3.52KK77 pKa = 11.18TGFPLRR83 pKa = 11.84FWYY86 pKa = 10.26LKK88 pKa = 10.62RR89 pKa = 11.84LVNDD93 pKa = 3.26NPRR96 pKa = 11.84ALLTLLTYY104 pKa = 7.99TRR106 pKa = 11.84RR107 pKa = 11.84IVPNKK112 pKa = 9.83SEE114 pKa = 4.06SKK116 pKa = 10.54ARR118 pKa = 11.84IVKK121 pKa = 10.48LSTITDD127 pKa = 3.97PYY129 pKa = 9.27KK130 pKa = 10.86GKK132 pKa = 10.9VYY134 pKa = 9.45TIPKK138 pKa = 9.12WFILDD143 pKa = 4.68FISKK147 pKa = 10.5YY148 pKa = 10.21NLSSTKK154 pKa = 10.11PIYY157 pKa = 9.91TDD159 pKa = 3.03NDD161 pKa = 3.58HH162 pKa = 6.38YY163 pKa = 11.56LSIKK167 pKa = 10.25GSPNGKK173 pKa = 10.05ASMSSLYY180 pKa = 10.75SIISFNSSNIRR191 pKa = 11.84YY192 pKa = 8.62LFNIVGDD199 pKa = 3.95YY200 pKa = 10.94QLVLNKK206 pKa = 9.73FYY208 pKa = 11.04QDD210 pKa = 3.42LSQFYY215 pKa = 8.96TKK217 pKa = 10.69YY218 pKa = 10.41INRR221 pKa = 11.84DD222 pKa = 3.03KK223 pKa = 11.39LGLGKK228 pKa = 10.47LSIVHH233 pKa = 6.88DD234 pKa = 4.31PEE236 pKa = 4.72LKK238 pKa = 9.94EE239 pKa = 4.09RR240 pKa = 11.84VIAMVDD246 pKa = 3.38YY247 pKa = 7.55TTQFALRR254 pKa = 11.84PIHH257 pKa = 7.01NILLNNLSKK266 pKa = 10.94LPCDD270 pKa = 3.22RR271 pKa = 11.84TFTQDD276 pKa = 5.3PFHH279 pKa = 7.03KK280 pKa = 10.3WNDD283 pKa = 3.45DD284 pKa = 3.18HH285 pKa = 8.39KK286 pKa = 10.77EE287 pKa = 4.1RR288 pKa = 11.84YY289 pKa = 9.21HH290 pKa = 8.71SLDD293 pKa = 3.16LSAATDD299 pKa = 3.8RR300 pKa = 11.84FPIFLQQKK308 pKa = 9.61LISLIFNDD316 pKa = 4.47YY317 pKa = 11.18EE318 pKa = 4.63FGKK321 pKa = 10.07NWRR324 pKa = 11.84NLLVDD329 pKa = 4.32RR330 pKa = 11.84NYY332 pKa = 11.02DD333 pKa = 3.55YY334 pKa = 11.47QGISYY339 pKa = 9.07RR340 pKa = 11.84YY341 pKa = 9.63SVGQPMGAYY350 pKa = 8.28TSWAAFTLTHH360 pKa = 6.41HH361 pKa = 6.96LVVHH365 pKa = 6.47WAAEE369 pKa = 3.99LAGLKK374 pKa = 10.05NFKK377 pKa = 10.48DD378 pKa = 4.22YY379 pKa = 10.95IILGDD384 pKa = 5.07DD385 pKa = 3.69IVIKK389 pKa = 10.38NNKK392 pKa = 7.75VAQIYY397 pKa = 9.81INLMTKK403 pKa = 9.49WGVDD407 pKa = 2.8ISLSKK412 pKa = 9.15THH414 pKa = 6.07VSYY417 pKa = 8.85DD418 pKa = 3.15TYY420 pKa = 11.38EE421 pKa = 3.85FAKK424 pKa = 10.34RR425 pKa = 11.84WIKK428 pKa = 10.26NGKK431 pKa = 8.17EE432 pKa = 3.41ISGISLKK439 pKa = 11.08GILTNIRR446 pKa = 11.84HH447 pKa = 4.95IHH449 pKa = 4.58VVYY452 pKa = 10.46MNIFTYY458 pKa = 9.72LQRR461 pKa = 11.84IPSLNVDD468 pKa = 3.18ILTCVGKK475 pKa = 10.75LYY477 pKa = 10.79GYY479 pKa = 10.64LLIRR483 pKa = 11.84NRR485 pKa = 11.84IKK487 pKa = 10.84SPNTIKK493 pKa = 10.64RR494 pKa = 11.84SLYY497 pKa = 10.3DD498 pKa = 3.01FHH500 pKa = 8.96HH501 pKa = 7.1SIRR504 pKa = 11.84YY505 pKa = 9.15SFGLLNYY512 pKa = 10.15EE513 pKa = 4.34EE514 pKa = 4.22IRR516 pKa = 11.84NYY518 pKa = 10.6LHH520 pKa = 7.18NKK522 pKa = 9.52FPFDD526 pKa = 4.46NYY528 pKa = 9.8YY529 pKa = 10.73AWPEE533 pKa = 3.93RR534 pKa = 11.84LVHH537 pKa = 6.53SKK539 pKa = 10.86LNEE542 pKa = 3.73IFKK545 pKa = 11.23LEE547 pKa = 4.06MVEE550 pKa = 4.31SAKK553 pKa = 10.88SFSKK557 pKa = 11.09DD558 pKa = 3.23FMNQSTMLINTVTDD572 pKa = 3.53NEE574 pKa = 4.98IMVQWPLYY582 pKa = 9.71KK583 pKa = 10.69GFMNHH588 pKa = 5.99IEE590 pKa = 4.08KK591 pKa = 10.79LKK593 pKa = 10.8DD594 pKa = 3.63YY595 pKa = 10.59IKK597 pKa = 10.8SKK599 pKa = 10.68QNQHH603 pKa = 7.29DD604 pKa = 3.94IDD606 pKa = 5.4LLDD609 pKa = 3.89LMQNLRR615 pKa = 11.84FQNLDD620 pKa = 3.73SIVKK624 pKa = 10.07KK625 pKa = 10.62LRR627 pKa = 11.84NSYY630 pKa = 9.95TNLIMLDD637 pKa = 4.1KK638 pKa = 10.19FWKK641 pKa = 9.58SAFNRR646 pKa = 11.84EE647 pKa = 3.94YY648 pKa = 10.81RR649 pKa = 11.84DD650 pKa = 3.59LEE652 pKa = 4.2RR653 pKa = 11.84EE654 pKa = 4.17SILTIEE660 pKa = 4.26KK661 pKa = 10.24QEE663 pKa = 4.42SNMMSRR669 pKa = 11.84IWDD672 pKa = 3.39MALSYY677 pKa = 8.91RR678 pKa = 11.84TSPMSYY684 pKa = 8.62STLTFEE690 pKa = 4.75TDD692 pKa = 2.9SEE694 pKa = 5.25FYY696 pKa = 10.31MMPSIWDD703 pKa = 3.62MASSSTTGPKK713 pKa = 10.29PFTTATFKK721 pKa = 10.83TDD723 pKa = 3.58FVGLSTFDD731 pKa = 4.49DD732 pKa = 4.2KK733 pKa = 11.56LLKK736 pKa = 10.66DD737 pKa = 4.24LEE739 pKa = 4.22NLKK742 pKa = 10.03IDD744 pKa = 3.12ITLRR748 pKa = 11.84TGKK751 pKa = 8.58YY752 pKa = 7.22TNKK755 pKa = 8.67TQPLEE760 pKa = 4.4SKK762 pKa = 9.78IHH764 pKa = 4.41QHH766 pKa = 6.58PIEE769 pKa = 5.01CIEE772 pKa = 4.51DD773 pKa = 3.45NKK775 pKa = 10.46VPNFNNIKK783 pKa = 10.42

Molecular weight: 92.25 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1	0	1	783	783	783	783.0	92.25

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
3.065 ± 0.0	0.511 ± 0.0	6.003 ± 0.0	3.704 ± 0.0	5.109 ± 0.0	3.448 ± 0.0	2.937 ± 0.0	9.068 ± 0.0	8.812 ± 0.0	11.111 ± 0.0

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.81 ± 0.0	7.535 ± 0.0	3.321 ± 0.0	2.682 ± 0.0	4.725 ± 0.0	7.663 ± 0.0	6.003 ± 0.0	3.831 ± 0.0	1.66 ± 0.0	6.003 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski