Streptococcus phage 20617

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6LMV9|W6LMV9_9CAUD Uncharacterized protein OS=Streptococcus phage 20617 OX=1392231 GN=e31 PE=4 SV=1
MM1 pKa = 7.52SVQQSIVNWFVNHH14 pKa = 6.82RR15 pKa = 11.84GKK17 pKa = 9.04LTYY20 pKa = 11.06SMYY23 pKa = 10.84GSRR26 pKa = 11.84NGSDD30 pKa = 2.98GTADD34 pKa = 3.38CSGSISQALKK44 pKa = 9.48EE45 pKa = 4.33AGVGIQGLPSTVTLGQQLANNGFYY69 pKa = 10.23RR70 pKa = 11.84VSRR73 pKa = 11.84NQDD76 pKa = 2.27WDD78 pKa = 3.64ALAGDD83 pKa = 5.28IILMSWGVDD92 pKa = 3.24MSQSGGAGGHH102 pKa = 5.81VGVMMDD108 pKa = 3.26SVNFISCDD116 pKa = 3.3YY117 pKa = 8.82STQGAVGQAINTYY130 pKa = 8.91PWNDD134 pKa = 3.25YY135 pKa = 9.98YY136 pKa = 11.66ASNKK140 pKa = 8.26PAYY143 pKa = 9.41IEE145 pKa = 3.78VWRR148 pKa = 11.84YY149 pKa = 10.1ADD151 pKa = 5.12SAPQTNNQANTAVSPQQKK169 pKa = 10.31AYY171 pKa = 9.83YY172 pKa = 8.44QANEE176 pKa = 3.84VKK178 pKa = 9.93YY179 pKa = 11.2VNGIWQIKK187 pKa = 9.16CDD189 pKa = 3.74YY190 pKa = 9.43LVPVGFDD197 pKa = 2.86WTEE200 pKa = 3.58NGIPVAMVNWVDD212 pKa = 4.37ADD214 pKa = 4.05GNDD217 pKa = 3.81LADD220 pKa = 5.2GADD223 pKa = 3.38QDD225 pKa = 4.72FKK227 pKa = 11.75AGMFFSFEE235 pKa = 3.95GDD237 pKa = 3.2EE238 pKa = 4.36TNITDD243 pKa = 3.78TGNGGYY249 pKa = 10.36YY250 pKa = 9.99GGYY253 pKa = 8.35YY254 pKa = 8.81WRR256 pKa = 11.84QFEE259 pKa = 4.19FGQFGTVWLSCWNKK273 pKa = 10.66DD274 pKa = 3.89DD275 pKa = 5.14LVNYY279 pKa = 7.38YY280 pKa = 8.26QQ281 pKa = 4.22

Molecular weight: 31.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6LMT1|W6LMT1_9CAUD Phage protein OS=Streptococcus phage 20617 OX=1392231 GN=php7 PE=4 SV=1
MM1 pKa = 7.83ADD3 pKa = 3.75FGVSLLEE10 pKa = 4.01ARR12 pKa = 11.84RR13 pKa = 11.84MTLKK17 pKa = 10.54EE18 pKa = 3.35MRR20 pKa = 11.84LYY22 pKa = 10.44QKK24 pKa = 10.59AYY26 pKa = 9.79KK27 pKa = 10.05KK28 pKa = 10.52RR29 pKa = 11.84FLNKK33 pKa = 9.43EE34 pKa = 3.69RR35 pKa = 11.84EE36 pKa = 4.18IYY38 pKa = 10.27QLAFLNRR45 pKa = 11.84LANATTRR52 pKa = 11.84DD53 pKa = 3.11GKK55 pKa = 10.61RR56 pKa = 11.84YY57 pKa = 9.56HH58 pKa = 6.45FEE60 pKa = 3.92KK61 pKa = 11.17FEE63 pKa = 4.24DD64 pKa = 4.34FYY66 pKa = 11.61NAKK69 pKa = 9.59EE70 pKa = 3.87RR71 pKa = 11.84ARR73 pKa = 11.84EE74 pKa = 3.84VLGEE78 pKa = 4.21KK79 pKa = 8.61ITNKK83 pKa = 10.26KK84 pKa = 9.81LLEE87 pKa = 4.02RR88 pKa = 11.84ARR90 pKa = 11.84NNLNYY95 pKa = 10.06KK96 pKa = 9.85KK97 pKa = 10.46EE98 pKa = 3.94RR99 pKa = 11.84GLLDD103 pKa = 3.22GRR105 pKa = 4.46

Molecular weight: 12.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

1

68

14848

38

1538

218.4

24.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.792 ± 0.955

0.512 ± 0.078

6.014 ± 0.374

7.166 ± 0.643

4.196 ± 0.186

6.674 ± 0.468

1.273 ± 0.15

6.782 ± 0.356

7.691 ± 0.506

8.102 ± 0.383

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.613 ± 0.235

5.502 ± 0.342

2.768 ± 0.196

4.189 ± 0.267

4.371 ± 0.299

6.796 ± 0.505

6.243 ± 0.309

6.102 ± 0.269

1.105 ± 0.174

4.108 ± 0.414
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski