Yellowstone Lake virophage 6

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Maveriviricetes; Priklausovirales; Lavidaviridae; unclassified Lavidaviridae

Average proteome isoelectric point is 7.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0RK71|A0A0A0RK71_9VIRU Putative primase-helicase OS=Yellowstone Lake virophage 6 OX=1557034 GN=YSLV6_ORF03 PE=4 SV=1
MM1 pKa = 8.02PDD3 pKa = 3.55LVTPEE8 pKa = 3.91YY9 pKa = 10.89QNYY12 pKa = 7.56TFKK15 pKa = 11.02KK16 pKa = 9.57DD17 pKa = 3.66LIGAFTLEE25 pKa = 4.15EE26 pKa = 4.12LQSEE30 pKa = 4.43FQEE33 pKa = 3.99ISNEE37 pKa = 3.68FFRR40 pKa = 11.84IKK42 pKa = 10.51EE43 pKa = 4.0LIYY46 pKa = 11.06DD47 pKa = 3.87NLEE50 pKa = 3.93EE51 pKa = 4.3SPEE54 pKa = 3.98IVVPEE59 pKa = 4.38MGLEE63 pKa = 4.25DD64 pKa = 4.72PDD66 pKa = 5.64FIDD69 pKa = 4.43KK70 pKa = 10.69LVYY73 pKa = 10.83DD74 pKa = 4.91KK75 pKa = 9.91DD76 pKa = 3.96TPLHH80 pKa = 5.84IKK82 pKa = 10.15KK83 pKa = 9.59LANYY87 pKa = 7.84FVSLRR92 pKa = 11.84VEE94 pKa = 4.07RR95 pKa = 11.84NFIDD99 pKa = 3.8SVIDD103 pKa = 3.1MSRR106 pKa = 11.84PKK108 pKa = 10.82LEE110 pKa = 5.1ADD112 pKa = 4.98DD113 pKa = 4.08IDD115 pKa = 4.07EE116 pKa = 4.55SFEE119 pKa = 4.12ALPTTGLSDD128 pKa = 3.49SKK130 pKa = 10.89KK131 pKa = 9.75IEE133 pKa = 4.18LEE135 pKa = 3.98QEE137 pKa = 4.09VQSILDD143 pKa = 4.33IINEE147 pKa = 4.14TDD149 pKa = 3.79VEE151 pKa = 4.2LQEE154 pKa = 4.66NAKK157 pKa = 10.4QYY159 pKa = 11.24EE160 pKa = 3.96KK161 pKa = 11.08DD162 pKa = 3.49LNLFEE167 pKa = 5.35EE168 pKa = 5.33DD169 pKa = 3.18IKK171 pKa = 11.31KK172 pKa = 10.51SGIEE176 pKa = 3.88IPPDD180 pKa = 3.39GLNADD185 pKa = 5.06FIEE188 pKa = 4.93TIQEE192 pKa = 4.09KK193 pKa = 10.79LSDD196 pKa = 3.58KK197 pKa = 10.82SQAILSQLIDD207 pKa = 3.42IAEE210 pKa = 4.31DD211 pKa = 3.56TLQKK215 pKa = 9.84KK216 pKa = 6.5TLKK219 pKa = 10.23EE220 pKa = 3.93EE221 pKa = 4.17KK222 pKa = 9.81KK223 pKa = 10.34QEE225 pKa = 3.73VDD227 pKa = 3.26ILIQQIEE234 pKa = 4.33EE235 pKa = 4.42SPVNIDD241 pKa = 3.88DD242 pKa = 3.95LKK244 pKa = 11.51NYY246 pKa = 10.02IFINRR251 pKa = 11.84STNEE255 pKa = 3.51FPYY258 pKa = 9.83YY259 pKa = 8.63TLEE262 pKa = 4.03YY263 pKa = 9.55KK264 pKa = 9.7KK265 pKa = 10.75QKK267 pKa = 9.81IDD269 pKa = 3.5EE270 pKa = 4.38LEE272 pKa = 4.14KK273 pKa = 8.93EE274 pKa = 4.22RR275 pKa = 11.84KK276 pKa = 9.7RR277 pKa = 11.84IGEE280 pKa = 4.17LIINEE285 pKa = 4.2VKK287 pKa = 10.69KK288 pKa = 11.11KK289 pKa = 10.21EE290 pKa = 3.92FF291 pKa = 3.7

Molecular weight: 34.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0RK92|A0A0A0RK92_9VIRU Uncharacterized protein OS=Yellowstone Lake virophage 6 OX=1557034 GN=YSLV6_ORF28 PE=4 SV=1
MM1 pKa = 7.1SAVRR5 pKa = 11.84GYY7 pKa = 11.2DD8 pKa = 3.14NLYY11 pKa = 10.0NEE13 pKa = 4.88HH14 pKa = 6.55VNDD17 pKa = 3.81IALMNYY23 pKa = 9.93NKK25 pKa = 10.61GIANRR30 pKa = 11.84LRR32 pKa = 11.84MQEE35 pKa = 3.51QAMQSKK41 pKa = 10.61LPFKK45 pKa = 10.44EE46 pKa = 3.93PQLLSGGVRR55 pKa = 11.84ASNSIVAGTTAEE67 pKa = 4.31PPSTLAVGGRR77 pKa = 11.84AFRR80 pKa = 11.84TFTGKK85 pKa = 7.74EE86 pKa = 4.1TPSQGGVNRR95 pKa = 11.84LKK97 pKa = 10.48KK98 pKa = 10.5AKK100 pKa = 10.15NGPHH104 pKa = 4.91TQKK107 pKa = 10.42IPWAMHH113 pKa = 5.38LTSQKK118 pKa = 8.72WRR120 pKa = 11.84VWVLKK125 pKa = 10.01VEE127 pKa = 4.01WFIVKK132 pKa = 9.79IYY134 pKa = 10.27KK135 pKa = 9.95KK136 pKa = 9.95HH137 pKa = 5.22

Molecular weight: 15.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

29

0

29

7356

56

1070

253.7

28.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.568 ± 0.666

1.088 ± 0.232

5.166 ± 0.596

5.424 ± 0.968

3.806 ± 0.497

5.914 ± 0.998

0.843 ± 0.183

8.877 ± 0.344

9.122 ± 1.631

8.904 ± 0.353

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.672 ± 0.239

8.021 ± 0.339

3.399 ± 0.305

3.29 ± 0.263

3.263 ± 0.351

8.333 ± 1.015

7.817 ± 1.537

4.948 ± 0.315

0.816 ± 0.1

4.731 ± 0.395
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski