Zhizhongheella caldifontis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Zhizhongheella

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3372 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S5STL0|A0A2S5STL0_9BURK Helix-turn-helix transcriptional regulator OS=Zhizhongheella caldifontis OX=1452508 GN=C1704_10400 PE=4 SV=1
MM1 pKa = 7.53AGSYY5 pKa = 10.27TDD7 pKa = 3.12IAGNSGSAASLTLAADD23 pKa = 3.77IAAPVVDD30 pKa = 5.8LDD32 pKa = 3.97ASSPGTGYY40 pKa = 11.0VVTFTEE46 pKa = 4.09NGSPVSIGDD55 pKa = 3.37IDD57 pKa = 5.76LSITDD62 pKa = 4.34ADD64 pKa = 3.91STTLSGATITLSNPQADD81 pKa = 3.89DD82 pKa = 3.58VLAVGSMPAGITATVVGNVVTLSGTASLADD112 pKa = 3.54YY113 pKa = 8.88EE114 pKa = 4.23AALRR118 pKa = 11.84SISFEE123 pKa = 4.21NTSEE127 pKa = 4.72APDD130 pKa = 3.59TTPRR134 pKa = 11.84TISVSVVDD142 pKa = 4.37ANGNASAPAVATVQVVSVDD161 pKa = 3.75DD162 pKa = 4.73APVHH166 pKa = 6.06ALPGPVTMLEE176 pKa = 4.11DD177 pKa = 3.5SQIYY181 pKa = 9.26LLPGTYY187 pKa = 8.59VTDD190 pKa = 3.5IDD192 pKa = 4.39SDD194 pKa = 4.85LITTTVSVPVGTLVLGSTDD213 pKa = 3.06GVTVSGNGTGVVVLSGSPSAVNAALIWTTYY243 pKa = 9.54TPPQDD248 pKa = 3.59YY249 pKa = 10.96NGVVDD254 pKa = 3.68ITVTTTDD261 pKa = 3.07GTSTVTDD268 pKa = 3.86SLNVTITPVNDD279 pKa = 3.23APVAVDD285 pKa = 3.7DD286 pKa = 4.42TFSTAEE292 pKa = 4.09DD293 pKa = 3.56TAITITPAQLLANDD307 pKa = 3.93IDD309 pKa = 4.06VDD311 pKa = 3.96GDD313 pKa = 3.94ALSVATIHH321 pKa = 6.69NITNGSISIVGGNIVFTPNPNYY343 pKa = 10.24HH344 pKa = 6.39GPASFDD350 pKa = 3.43YY351 pKa = 10.14TISDD355 pKa = 3.3GRR357 pKa = 11.84GGFSTARR364 pKa = 11.84ATIDD368 pKa = 3.4VLSVDD373 pKa = 5.06DD374 pKa = 4.89LPEE377 pKa = 4.18TADD380 pKa = 3.57VNASGAEE387 pKa = 3.94DD388 pKa = 3.74SVIAIALSGSDD399 pKa = 3.37VDD401 pKa = 4.88NVITGYY407 pKa = 11.08VIGTLPANGVLYY419 pKa = 10.48RR420 pKa = 11.84DD421 pKa = 3.5AALTQVVSAGDD432 pKa = 3.69VVSEE436 pKa = 4.0TTLYY440 pKa = 10.18FRR442 pKa = 11.84PADD445 pKa = 3.3NWNGNTSFQYY455 pKa = 10.76AAQNDD460 pKa = 4.05AGISDD465 pKa = 4.27PTPATVVITVTPVNDD480 pKa = 3.37APVAVNDD487 pKa = 4.03SFTTAEE493 pKa = 4.3DD494 pKa = 3.52TAITITPAQLLANDD508 pKa = 3.95IDD510 pKa = 3.93VDD512 pKa = 4.14GDD514 pKa = 3.9VLSVATIHH522 pKa = 6.68NITNGSISIVGGNIVFTPNPNYY544 pKa = 10.24HH545 pKa = 6.39GPASFDD551 pKa = 3.44YY552 pKa = 10.34TISDD556 pKa = 3.74GQGGFSTATATISVTPVNDD575 pKa = 3.27APQAVDD581 pKa = 4.86DD582 pKa = 5.09GPSMLTGLRR591 pKa = 11.84GEE593 pKa = 4.15YY594 pKa = 9.27FAYY597 pKa = 10.23VEE599 pKa = 4.64GMSGPNLTSVQQVRR613 pKa = 11.84AFADD617 pKa = 3.44ANTADD622 pKa = 3.61AVFNATTLTYY632 pKa = 11.09NEE634 pKa = 4.19INGNLGADD642 pKa = 3.43GRR644 pKa = 11.84LQAFLGSDD652 pKa = 3.42AASLSVDD659 pKa = 3.9PPNSSDD665 pKa = 3.68AILRR669 pKa = 11.84FTGTLDD675 pKa = 3.7LAAGTYY681 pKa = 9.14ILRR684 pKa = 11.84VRR686 pKa = 11.84ADD688 pKa = 3.52DD689 pKa = 5.11GYY691 pKa = 11.57SIMIDD696 pKa = 3.24GVVVAEE702 pKa = 4.43VNANQAPTTTTHH714 pKa = 7.18AAFTLPTGGPHH725 pKa = 7.01SIDD728 pKa = 3.45IIYY731 pKa = 9.58WDD733 pKa = 3.41QGGQAVFGVEE743 pKa = 3.96LSADD747 pKa = 3.63NGSTFQPLGQYY758 pKa = 7.2TLRR761 pKa = 11.84HH762 pKa = 5.2PTYY765 pKa = 8.3TTAEE769 pKa = 4.12DD770 pKa = 3.58TALVISTATLLANDD784 pKa = 4.36TDD786 pKa = 3.86VDD788 pKa = 4.18GDD790 pKa = 3.98PLSVVSVQDD799 pKa = 3.55AVNGTVTYY807 pKa = 10.59SGGQIIFTPAPDD819 pKa = 3.62YY820 pKa = 10.96HH821 pKa = 7.93GPASFTYY828 pKa = 9.47TVSDD832 pKa = 4.1GNGGTSTATVSLNILSVNDD851 pKa = 3.68MPTTANVTATGAEE864 pKa = 4.13DD865 pKa = 3.46SVLAVTLAGADD876 pKa = 2.9IDD878 pKa = 4.12GTVTGFVIQNLPANGTLYY896 pKa = 11.15ADD898 pKa = 4.19AARR901 pKa = 11.84TQVISAGSVVPGPVVYY917 pKa = 9.02FQPSANWNGSTTFQYY932 pKa = 11.13AARR935 pKa = 11.84DD936 pKa = 3.91NEE938 pKa = 4.31GGTDD942 pKa = 3.7STPATATLTVTPVNDD957 pKa = 3.34APVNVVPGLQTFNEE971 pKa = 4.2DD972 pKa = 3.03TSRR975 pKa = 11.84VFSVANGNAISVADD989 pKa = 4.05IDD991 pKa = 4.39SASLTTSLSVSHH1003 pKa = 6.11GTLTLATTSGLTVSGNGTGTVTLSGSAAAINAALNGTTYY1042 pKa = 10.33TPAANVHH1049 pKa = 5.86GATTLTVTTSDD1060 pKa = 3.5GSLSTTSTVPMNVVAVADD1078 pKa = 4.24TPLLGAQEE1086 pKa = 4.98IIQVLNPGSTMISTGAGMPSNFTRR1110 pKa = 11.84GTNHH1114 pKa = 6.77GDD1116 pKa = 3.3GVSAANLEE1124 pKa = 4.38AEE1126 pKa = 4.73LGLAPGALSQFNPPGSHH1143 pKa = 6.76AGNVTAIDD1151 pKa = 4.07GKK1153 pKa = 9.68LTTANYY1159 pKa = 10.0SLSAGTTVNFNWSFLNGEE1177 pKa = 4.63DD1178 pKa = 3.89LDD1180 pKa = 4.37SEE1182 pKa = 4.15IRR1184 pKa = 11.84AGYY1187 pKa = 9.25NDD1189 pKa = 4.35LVILVVTDD1197 pKa = 3.58PAGNRR1202 pKa = 11.84QAIQVTSSEE1211 pKa = 4.13QAGASTGTLNGTYY1224 pKa = 10.08AYY1226 pKa = 7.69TAASAGSYY1234 pKa = 10.01KK1235 pKa = 10.18FDD1237 pKa = 2.96WVIVNGRR1244 pKa = 11.84DD1245 pKa = 3.12AGKK1248 pKa = 10.29DD1249 pKa = 3.35SRR1251 pKa = 11.84LHH1253 pKa = 5.28LTSTSFSTSGSAHH1266 pKa = 6.57GAPVALALFAALTDD1280 pKa = 3.85TDD1282 pKa = 3.91GSEE1285 pKa = 4.28SLSVTVAGVPSGARR1299 pKa = 11.84LSSGTNLGSGVWSLSPADD1317 pKa = 4.32LPGLQLLPASGYY1329 pKa = 9.32TGTINLNVTATATEE1343 pKa = 3.99ASNGSVASTSRR1354 pKa = 11.84SISITIAEE1362 pKa = 4.43TGNTVHH1368 pKa = 6.88SGSQSGDD1375 pKa = 3.65SVSGASGNSSDD1386 pKa = 5.7LIHH1389 pKa = 7.19GYY1391 pKa = 10.38AGNDD1395 pKa = 3.66TINAGNGHH1403 pKa = 6.67DD1404 pKa = 4.31LVHH1407 pKa = 6.84GGTGNDD1413 pKa = 3.55TLNGEE1418 pKa = 4.69DD1419 pKa = 3.92GHH1421 pKa = 7.1DD1422 pKa = 3.67VLYY1425 pKa = 11.15GGAGNDD1431 pKa = 3.89TLNGGAGNDD1440 pKa = 3.33ILVGGTGNDD1449 pKa = 3.52TLTGGTGADD1458 pKa = 3.26VFRR1461 pKa = 11.84WHH1463 pKa = 6.77FGDD1466 pKa = 3.79GGVVGTPAVDD1476 pKa = 4.24TITDD1480 pKa = 4.41FNNSTGPQGDD1490 pKa = 3.87VLDD1493 pKa = 4.97LRR1495 pKa = 11.84DD1496 pKa = 3.79LLVGEE1501 pKa = 4.31SHH1503 pKa = 6.9GNLSNYY1509 pKa = 10.03LHH1511 pKa = 7.13FSTSGSGASTTTTISISTTGGFAGGFSASAVDD1543 pKa = 5.68QVIQLNNVDD1552 pKa = 4.45LVQSFTSDD1560 pKa = 3.12QQIIQDD1566 pKa = 4.06LLARR1570 pKa = 11.84GKK1572 pKa = 10.41LLSDD1576 pKa = 3.94

Molecular weight: 158.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S5SXU2|A0A2S5SXU2_9BURK NAD-dependent succinate-semialdehyde dehydrogenase OS=Zhizhongheella caldifontis OX=1452508 GN=C1704_04290 PE=4 SV=1
MM1 pKa = 7.19KK2 pKa = 10.25RR3 pKa = 11.84SYY5 pKa = 10.63QPSKK9 pKa = 9.66VRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.65GFLVRR21 pKa = 11.84MKK23 pKa = 9.67TKK25 pKa = 10.48GGRR28 pKa = 11.84KK29 pKa = 9.03VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3372

0

3372

1112190

28

2190

329.8

35.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.84 ± 0.057

0.893 ± 0.014

5.2 ± 0.027

5.618 ± 0.037

3.372 ± 0.026

8.281 ± 0.034

2.458 ± 0.025

3.977 ± 0.035

2.756 ± 0.035

11.19 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.344 ± 0.019

2.209 ± 0.022

5.527 ± 0.031

3.998 ± 0.024

7.781 ± 0.04

4.918 ± 0.026

4.951 ± 0.028

7.928 ± 0.032

1.624 ± 0.023

2.132 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski