Raphanus sativus cryptic virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; unclassified Partitiviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q52UL1|Q52UL1_9VIRU RNA-dependent RNA polymerase OS=Raphanus sativus cryptic virus 1 OX=348449 PE=4 SV=2
MM1 pKa = 7.69AHH3 pKa = 6.35RR4 pKa = 11.84TPTNAPALPQVDD16 pKa = 5.17GINPNLPPNDD26 pKa = 4.32PGTVAAAPNARR37 pKa = 11.84NLHH40 pKa = 5.71LEE42 pKa = 4.1RR43 pKa = 11.84EE44 pKa = 4.21QEE46 pKa = 3.88ARR48 pKa = 11.84QDD50 pKa = 2.98RR51 pKa = 11.84AAAAFVPNTRR61 pKa = 11.84FAVRR65 pKa = 11.84RR66 pKa = 11.84PTIRR70 pKa = 11.84PSPAHH75 pKa = 6.12SAPDD79 pKa = 3.52LEE81 pKa = 4.54TALFDD86 pKa = 4.06SAVNHH91 pKa = 6.79PPTPVSYY98 pKa = 9.83PSTSSYY104 pKa = 10.68IPNFTSAFYY113 pKa = 10.71YY114 pKa = 9.65LNKK117 pKa = 9.34MDD119 pKa = 4.32SLMVQTLNWTNNCSGWVPPYY139 pKa = 8.97SQIYY143 pKa = 10.19ISMLLYY149 pKa = 9.74LQVMRR154 pKa = 11.84AMKK157 pKa = 10.17KK158 pKa = 10.51AGVLRR163 pKa = 11.84PNSEE167 pKa = 3.92LSHH170 pKa = 6.63LFNEE174 pKa = 4.71MSTIFPFEE182 pKa = 4.13SLMVPGPLVNLFEE195 pKa = 5.63NITAFRR201 pKa = 11.84PLQTDD206 pKa = 2.97SFGNVTPFLPAEE218 pKa = 4.63PGWSNATFFAPNGSLVRR235 pKa = 11.84HH236 pKa = 6.49LPHH239 pKa = 7.04IPALISRR246 pKa = 11.84LRR248 pKa = 11.84RR249 pKa = 11.84ICEE252 pKa = 3.81TASEE256 pKa = 4.26NGLNDD261 pKa = 3.08ISFSAHH267 pKa = 3.93HH268 pKa = 6.84HH269 pKa = 5.35GPEE272 pKa = 4.63FISEE276 pKa = 4.28LFGHH280 pKa = 6.79ICDD283 pKa = 4.2NDD285 pKa = 3.76LPEE288 pKa = 4.32QLLLLTPGLATSYY301 pKa = 11.03SGTLYY306 pKa = 10.71LWRR309 pKa = 11.84QARR312 pKa = 11.84SQLQRR317 pKa = 11.84SLFPEE322 pKa = 4.32ALTVNDD328 pKa = 3.95VVPNTWTSFLCLDD341 pKa = 4.78NDD343 pKa = 4.54DD344 pKa = 4.7SWFSPLAAMMNKK356 pKa = 9.09YY357 pKa = 8.98CQYY360 pKa = 8.41WHH362 pKa = 7.02GSAPLSSIPADD373 pKa = 3.67GSAAGSMICNEE384 pKa = 4.43LNGSSIYY391 pKa = 10.61RR392 pKa = 11.84LAQWTAPIMDD402 pKa = 4.7PPADD406 pKa = 3.75EE407 pKa = 5.15DD408 pKa = 4.04APPVIAEE415 pKa = 3.89PGYY418 pKa = 10.69YY419 pKa = 9.57YY420 pKa = 9.89PNPNPVLIFDD430 pKa = 4.33ARR432 pKa = 11.84TCIEE436 pKa = 5.3DD437 pKa = 3.05ISSAHH442 pKa = 6.48MFSAMTFHH450 pKa = 7.54PNLIPYY456 pKa = 7.76GANRR460 pKa = 11.84ANFLKK465 pKa = 10.75GQFWNCHH472 pKa = 5.33PPSYY476 pKa = 10.08SAPAHH481 pKa = 4.59QVYY484 pKa = 8.85PAVSALIARR493 pKa = 11.84EE494 pKa = 3.92YY495 pKa = 10.76HH496 pKa = 6.41SEE498 pKa = 4.25TNLSSDD504 pKa = 3.33KK505 pKa = 11.34

Molecular weight: 55.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q52UL1|Q52UL1_9VIRU RNA-dependent RNA polymerase OS=Raphanus sativus cryptic virus 1 OX=348449 PE=4 SV=2
MM1 pKa = 7.77SNLEE5 pKa = 3.96YY6 pKa = 10.79LGLDD10 pKa = 3.51HH11 pKa = 7.33HH12 pKa = 6.96WPAIPRR18 pKa = 11.84FAKK21 pKa = 10.55SPNDD25 pKa = 3.16WYY27 pKa = 10.56FACQQLVRR35 pKa = 11.84SSICLYY41 pKa = 10.94SSLLGSNDD49 pKa = 3.03TDD51 pKa = 3.75TVLNGYY57 pKa = 8.48YY58 pKa = 9.9RR59 pKa = 11.84SHH61 pKa = 7.9ADD63 pKa = 3.29EE64 pKa = 4.63DD65 pKa = 3.94TAEE68 pKa = 4.12QFFMRR73 pKa = 11.84YY74 pKa = 9.22DD75 pKa = 3.49VEE77 pKa = 4.22PFDD80 pKa = 5.19IIKK83 pKa = 10.72DD84 pKa = 3.69NYY86 pKa = 9.61YY87 pKa = 10.46SQAFDD92 pKa = 3.88TVTEE96 pKa = 4.4WFRR99 pKa = 11.84PSAPIHH105 pKa = 5.83PVHH108 pKa = 5.95FTDD111 pKa = 4.69VRR113 pKa = 11.84WYY115 pKa = 8.09PWKK118 pKa = 10.35ISTSAEE124 pKa = 3.67RR125 pKa = 11.84PFTHH129 pKa = 7.86DD130 pKa = 3.63PLLKK134 pKa = 10.59KK135 pKa = 10.17KK136 pKa = 10.36VQLSKK141 pKa = 11.11QLGLLDD147 pKa = 3.59NARR150 pKa = 11.84MSFHH154 pKa = 6.63NCYY157 pKa = 10.64NDD159 pKa = 2.49IFTYY163 pKa = 9.9CRR165 pKa = 11.84HH166 pKa = 5.16YY167 pKa = 9.06THH169 pKa = 6.74EE170 pKa = 4.48VKK172 pKa = 10.5DD173 pKa = 3.77ARR175 pKa = 11.84PVTLHH180 pKa = 7.02HH181 pKa = 7.16IDD183 pKa = 3.78LHH185 pKa = 6.06VKK187 pKa = 8.97PALVRR192 pKa = 11.84SGEE195 pKa = 4.01PPKK198 pKa = 10.33IRR200 pKa = 11.84TVFGVPKK207 pKa = 10.45SLIFAEE213 pKa = 5.95AMFFWPLFSDD223 pKa = 3.91YY224 pKa = 9.05FTNSEE229 pKa = 4.67TPLLWNYY236 pKa = 7.32EE237 pKa = 4.18TLNGGWYY244 pKa = 10.1RR245 pKa = 11.84LNDD248 pKa = 3.31EE249 pKa = 6.3FYY251 pKa = 10.64QQWQSFCTIFNLDD264 pKa = 2.84WSEE267 pKa = 3.84FDD269 pKa = 3.13MRR271 pKa = 11.84VYY273 pKa = 10.71FSMLDD278 pKa = 3.5DD279 pKa = 4.53CRR281 pKa = 11.84DD282 pKa = 3.35AVKK285 pKa = 10.5SYY287 pKa = 11.11FCFCGNYY294 pKa = 9.8CPTRR298 pKa = 11.84TYY300 pKa = 7.53PTCRR304 pKa = 11.84TNPQRR309 pKa = 11.84LQNLWNWIGTAYY321 pKa = 10.01KK322 pKa = 8.88DD323 pKa = 4.1TPCTTTTGKK332 pKa = 10.2VYY334 pKa = 10.48RR335 pKa = 11.84RR336 pKa = 11.84RR337 pKa = 11.84FAGMPSGIFCTQFWDD352 pKa = 3.81SFYY355 pKa = 10.73NCIMVVTTLEE365 pKa = 3.98ALGFRR370 pKa = 11.84ITDD373 pKa = 3.7RR374 pKa = 11.84YY375 pKa = 8.65FLKK378 pKa = 11.0VLGDD382 pKa = 3.75DD383 pKa = 4.1VIFGILKK390 pKa = 9.73HH391 pKa = 6.87IPISKK396 pKa = 9.29WADD399 pKa = 3.23FLQDD403 pKa = 3.48FSTEE407 pKa = 3.16ARR409 pKa = 11.84RR410 pKa = 11.84RR411 pKa = 11.84FNSKK415 pKa = 10.43LNSKK419 pKa = 9.94KK420 pKa = 10.5CGASSGIHH428 pKa = 5.55GAQVLSYY435 pKa = 9.9INWNGYY441 pKa = 7.42PKK443 pKa = 10.0RR444 pKa = 11.84DD445 pKa = 3.79SNQLLAQLLHH455 pKa = 6.58PKK457 pKa = 9.59SLRR460 pKa = 11.84DD461 pKa = 3.42TYY463 pKa = 10.58PRR465 pKa = 11.84LMARR469 pKa = 11.84AIGIYY474 pKa = 9.5YY475 pKa = 10.06ASCGDD480 pKa = 3.59PKK482 pKa = 10.44IRR484 pKa = 11.84PICNHH489 pKa = 6.58IYY491 pKa = 10.67SEE493 pKa = 4.1LKK495 pKa = 10.13YY496 pKa = 10.89AGFTPSSTGLHH507 pKa = 6.18GLFDD511 pKa = 5.16PNASIGFIEE520 pKa = 5.15LDD522 pKa = 3.46HH523 pKa = 6.72FPSEE527 pKa = 4.37NEE529 pKa = 4.03VTCRR533 pKa = 11.84LHH535 pKa = 7.03RR536 pKa = 11.84KK537 pKa = 8.4SKK539 pKa = 10.67RR540 pKa = 11.84SAEE543 pKa = 4.01LQALYY548 pKa = 9.73WPRR551 pKa = 11.84DD552 pKa = 3.48HH553 pKa = 7.15FLEE556 pKa = 4.45EE557 pKa = 4.48AGSSRR562 pKa = 11.84NCPLSFQVEE571 pKa = 4.79TII573 pKa = 3.51

Molecular weight: 66.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1078

505

573

539.0

61.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.514 ± 1.716

2.412 ± 0.549

5.288 ± 0.749

4.082 ± 0.051

6.03 ± 0.716

4.36 ± 0.265

3.432 ± 0.044

4.824 ± 0.179

2.968 ± 1.182

9.091 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.134 ± 0.424

5.844 ± 0.853

8.071 ± 1.741

3.061 ± 0.06

5.473 ± 0.61

8.442 ± 0.574

5.844 ± 0.33

4.082 ± 0.051

2.319 ± 0.356

4.731 ± 0.643
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski