Vagococcus elongatus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Vagococcus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2619 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A430AM88|A0A430AM88_9ENTE Glycine--tRNA ligase alpha subunit OS=Vagococcus elongatus OX=180344 GN=glyQ PE=3 SV=1
MM1 pKa = 7.6GEE3 pKa = 4.21HH4 pKa = 6.5HH5 pKa = 7.03HH6 pKa = 7.24HH7 pKa = 7.17DD8 pKa = 4.08HH9 pKa = 7.79DD10 pKa = 5.5HH11 pKa = 7.53DD12 pKa = 4.15CQCNHH17 pKa = 6.8DD18 pKa = 3.83HH19 pKa = 7.08HH20 pKa = 6.6EE21 pKa = 4.26HH22 pKa = 7.45DD23 pKa = 5.37FITLVDD29 pKa = 3.82EE30 pKa = 5.03EE31 pKa = 4.87GNEE34 pKa = 3.86ALYY37 pKa = 10.61EE38 pKa = 3.97ILLTIDD44 pKa = 3.63GEE46 pKa = 4.54EE47 pKa = 3.96EE48 pKa = 3.93FANKK52 pKa = 9.92QYY54 pKa = 11.56VLLYY58 pKa = 9.59PAGIDD63 pKa = 3.44EE64 pKa = 5.65DD65 pKa = 5.44DD66 pKa = 3.96DD67 pKa = 4.55TEE69 pKa = 4.59VDD71 pKa = 3.77LLAYY75 pKa = 9.53EE76 pKa = 4.62YY77 pKa = 10.41IEE79 pKa = 4.41SASGTEE85 pKa = 4.22GEE87 pKa = 4.74LKK89 pKa = 10.74NIEE92 pKa = 4.22TDD94 pKa = 3.79AEE96 pKa = 3.72WDD98 pKa = 3.57MIEE101 pKa = 4.49EE102 pKa = 4.26VFNTFAAEE110 pKa = 3.88QEE112 pKa = 4.43EE113 pKa = 4.44

Molecular weight: 13.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A430AV17|A0A430AV17_9ENTE ABC transporter ATP-binding protein OS=Vagococcus elongatus OX=180344 GN=CBF29_07190 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.14QPSKK9 pKa = 9.19RR10 pKa = 11.84KK11 pKa = 9.13HH12 pKa = 5.47KK13 pKa = 9.91RR14 pKa = 11.84VHH16 pKa = 6.02GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.73GRR39 pKa = 11.84KK40 pKa = 8.76VLSAA44 pKa = 4.05

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2619

0

2619

802562

27

2761

306.4

34.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.593 ± 0.047

0.707 ± 0.015

5.53 ± 0.039

7.82 ± 0.048

4.598 ± 0.041

6.665 ± 0.044

1.863 ± 0.02

7.797 ± 0.041

7.317 ± 0.045

9.684 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.746 ± 0.027

4.657 ± 0.038

3.294 ± 0.026

3.791 ± 0.032

3.86 ± 0.037

6.174 ± 0.035

5.514 ± 0.035

6.855 ± 0.037

0.87 ± 0.016

3.666 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski